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Genetic Insert: Bile Acid Inducible Nanoluc expression, pNBU1 backbone with BsaI sites
Vector Backbone Description: Vector Backbone:pNBU1; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32231334
Proper citation: RRID:Addgene_139387 Copy
Genetic Insert: IPTG inducible Nanoluc expression,
Vector Backbone Description: Vector Backbone:pNBU1; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32231334
Proper citation: RRID:Addgene_139385 Copy
Genetic Insert: Bile Acid inducible sgRNA-1 with PM1 driving Nanoluc expression (NOT Gate 1),
Vector Backbone Description: Vector Backbone:pNBU1; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32231334
Proper citation: RRID:Addgene_139388 Copy
Genetic Insert: Bile Acid inducible sgRNA-2 with PM2 driving Nanoluc expression (NOT Gate 2)
Vector Backbone Description: Vector Backbone:pNBU1; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32231334
Proper citation: RRID:Addgene_139389 Copy
Species: E. coli
Genetic Insert: Eco1: HEK4 targeting and editing ncRNA-gRNA (a1/a2 length: 27 v1), expressed constitutively from a pol III (H1) promoter
Vector Backbone Description: Vector Backbone:pFUGW-H1 (Addgene plasmid #25870); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34949838
Proper citation: RRID:Addgene_185000 Copy
Genetic Insert: Bile Acid inducible sgRNA-3 with PM3 driving Nanoluc expression (NOT Gate 3), pNBU1 backbone, AmpR, ErmR
Vector Backbone Description: Vector Backbone:pNBU1; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32231334
Proper citation: RRID:Addgene_139390 Copy
Genetic Insert: XOR circuit (A000 and P000 design) with aTC and Bile Acid as inputs
Vector Backbone Description: Vector Backbone:pNBU1; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32231334
Proper citation: RRID:Addgene_139393 Copy
Genetic Insert: Nanoluc placed on Output 2 of the 2in3out circuit (A001 and P002 design) with aTC and Bile Acid as inputs
Vector Backbone Description: Vector Backbone:pNBU1; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32231334
Proper citation: RRID:Addgene_139394 Copy
Genetic Insert: Bile Acid inducible sgRNA-4 with PM4 driving Nanoluc expression (NOT Gate 4)
Vector Backbone Description: Vector Backbone:pNBU1; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32231334
Proper citation: RRID:Addgene_139391 Copy
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31442407
Proper citation: RRID:Addgene_192148 Copy
Vector Backbone Description: Vector Backbone:Lenti; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31442407
Proper citation: RRID:Addgene_192147 Copy
Species: Synthetic
Genetic Insert: tagBFP
Vector Backbone Description: Vector Backbone:pMax; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24757057
Comments: For more information, see Piett, Pecen et al. 2021 Nat Protoc. (doi: 10.1038/s41596-021-00577-3).
Proper citation: RRID:Addgene_177824 Copy
Species: Synthetic
Genetic Insert: GFP_C289T
Vector Backbone Description: Vector Backbone:pMax; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24757057
Comments: For more information, see Chaim et al. 2017 PNAS (doi: 10.1073/pnas.1712032114) and Piett, Pecen et al. 2021 Nat Protoc. (doi: 10.1038/s41596-021-00577-3).
Proper citation: RRID:Addgene_177826 Copy
Species: Synthetic
Genetic Insert: mOrange_A215C
Vector Backbone Description: Vector Backbone:pMax; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24757057
Comments: For more information, see Chaim et al. 2017 PNAS (doi: 10.1073/pnas.1712032114) and Piett, Pecen et al. 2021 Nat Protoc. (doi: 10.1038/s41596-021-00577-3).
Proper citation: RRID:Addgene_177828 Copy
Species: Homo sapiens
Genetic Insert: CPSF4
Vector Backbone Description: Backbone Size:7357; Vector Backbone:E2.pEF5-DEST-FL-V5-MCP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32807991
Proper citation: RRID:Addgene_155478 Copy
Species: Homo sapiens
Genetic Insert: DDX27
Vector Backbone Description: Backbone Size:7357; Vector Backbone:E2.pEF5-DEST-FL-V5-MCP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32807991
Proper citation: RRID:Addgene_155514 Copy
Species: Homo sapiens
Genetic Insert: CPEB1
Vector Backbone Description: Backbone Size:7357; Vector Backbone:E2.pEF5-DEST-FL-V5-MCP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32807991
Proper citation: RRID:Addgene_155472 Copy
Genetic Insert: GFP-P2A-Blasticidin
Vector Backbone Description: Backbone Size:8066; Vector Backbone:pLenti; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35396484
Proper citation: RRID:Addgene_182230 Copy
Species: Synthetic
Genetic Insert: BE3
Vector Backbone Description: Backbone Marker:Mumberg et al. (Nucleic Acids Research, 1994, Vol. 22, No. 25); Backbone Size:6653; Vector Backbone:p415-GALL; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36326816
Proper citation: RRID:Addgene_172409 Copy
Species: Mus musculus
Genetic Insert: gRNA and CRISPIE Halo donor
Vector Backbone Description: Vector Backbone:pOC2; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35851300
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.01.02.474730v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_183446 Copy
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