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Species: Synthetic
Genetic Insert: enAsCas12f
Vector Backbone Description: Vector Backbone:de novo assembled; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37400536
Proper citation: RRID:Addgene_204637 Copy
Species: Mus musculus
Genetic Insert: ZMYND8
Vector Backbone Description: Vector Backbone:pT2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36692427
Proper citation: RRID:Addgene_201405 Copy
Species: Synthetic
Genetic Insert: NbV5-TEV219
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5126; Vector Backbone:pcDNA6/myc-His A; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37517699
Proper citation: RRID:Addgene_201481 Copy
Species: Synthetic
Genetic Insert: LgBit-NbALFA
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5428; Vector Backbone:pcDNA3.1+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37517699
Proper citation: RRID:Addgene_201484 Copy
Species: Synthetic
Genetic Insert: NbALFA-LgBit
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5502; Vector Backbone:pcDNA6/ V5-HisA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37517699
Proper citation: RRID:Addgene_201483 Copy
Species: Synthetic
Genetic Insert: GFP2-NbALFA
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5428; Vector Backbone:pcDNA3.1+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37517699
Proper citation: RRID:Addgene_201486 Copy
Species: Synthetic
Genetic Insert: NbAlfa-GFP2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5428; Vector Backbone:pcDNA6/myc-His A; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37517699
Proper citation: RRID:Addgene_201485 Copy
Species: Homo sapiens
Genetic Insert: Flag-hS1PR1
Vector Backbone Description: Vector Backbone:pCDH-Neo; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_203709 Copy
Genetic Insert: CouRS
Vector Backbone Description: Vector Backbone:pSUP-(MjYRS-6TRN); Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:33020184
Proper citation: RRID:Addgene_197930 Copy
Species: Homo sapiens
Genetic Insert: bArr2-ALFA
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5428; Vector Backbone:pcDNA3.1+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37517699
Proper citation: RRID:Addgene_201498 Copy
Species: Homo sapiens
Genetic Insert: V5-bArr1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5428; Vector Backbone:pcDNA3.1+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37517699
Proper citation: RRID:Addgene_201491 Copy
Species: Synthetic
Genetic Insert: HiBiT-NbV5
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5428; Vector Backbone:pcDNA3.1+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37517699
Proper citation: RRID:Addgene_201490 Copy
Species: Homo sapiens
Genetic Insert: bArr1-ALFA
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5428; Vector Backbone:pcDNA6/myc-His A; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37517699
Proper citation: RRID:Addgene_201496 Copy
Species: Mus musculus
Genetic Insert: Cre
Vector Backbone Description: Vector Backbone:AAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37354902
Proper citation: RRID:Addgene_203842 Copy
Species: Homo sapiens
Genetic Insert: TGFA
Vector Backbone Description: Vector Backbone:pBabepuro; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_189610 Copy
Species: Synthetic
Genetic Insert: jGCaMP7f
Vector Backbone Description: Vector Backbone:AAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34038743
Proper citation: RRID:Addgene_203848 Copy
Species: Homo sapiens
Genetic Insert: ACSL4 promoter (partial)
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4818; Vector Backbone:pGL3 basic; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36928314
Proper citation: RRID:Addgene_202514 Copy
Species: Mus musculus
Genetic Insert: dCre
Vector Backbone Description: Vector Backbone:AAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37354902
Proper citation: RRID:Addgene_203845 Copy
Species: Synthetic
Genetic Insert: BCD 8.5K
Vector Backbone Description: Vector Backbone:pUC; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37253269
Comments: This plasmid encodes a part for use in a heirarchical golden gate plasmid assembly system. The part insert is a bicistronic translational control element for regulating translation of protein-coding genes in E. coli. It is flanked by sites for the type IIS restriction enzyme BsaI and yields 5' and 3' overhangs of GGAG and TATG respectively. The backbone contains a pUC origin, a TEM-1 ampicillin resistance marker, and a ccdB marker such that the part plasmid does not carry through into higher order plasmid assemblies. The plasmid must be propagated in an E. coli strain which is resistant to ccdB.
Please visit https://www.biorxiv.org/content/10.1101/2023.02.09.527918v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_200050 Copy
Species: Mus musculus
Genetic Insert: Kir2.1
Vector Backbone Description: Vector Backbone:AAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37354902
Proper citation: RRID:Addgene_203840 Copy
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