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http://www.addgene.org/119739

Species: Homo sapiens
Genetic Insert: PSAM4 GlyR IRES eGFP
Vector Backbone Description: Vector Backbone:pCAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30872534

Proper citation: RRID:Addgene_119739 Copy   

•   Source: Addgene
  

http://www.addgene.org/119737

Vector Backbone Description: Vector Backbone:See supplemental files for plasmid details; Vector Types:; Bacterial Resistance:None
Defining Citation: PMID:30738800
Comments: Strain can be grown on LB without antibiotics. Resistant to: ampicillin, chloramphenicol, florfenicol, gentamicin, kanamycin, nalidixic acid, streptomycin, spectinomycin, sulphonamides, tobramycin, tetracycline, trimethoprim Please note that plasmids are stable in the absence of antibiotic selection. 39R861+ is resistant to nalidixic acid due to a chromosomal mutation. The remaining resistance determinants are plasmid-borne. 39R861+ contains six plasmids, outlined in the supplemental files.

Proper citation: RRID:Addgene_119737 Copy   

•   Source: Addgene
  

http://www.addgene.org/119735

Species: Rattus norvegicus
Genetic Insert: GABRG2/GABRD
Vector Backbone Description: Vector Backbone:pCDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22711532

Proper citation: RRID:Addgene_119735 Copy   

•   Source: Addgene
  

http://www.addgene.org/11969

Species: Homo sapiens
Genetic Insert: NEMOL329P
Vector Backbone Description: Backbone Size:5370; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16547522

Proper citation: RRID:Addgene_11969 Copy   

•   Source: Addgene
  

http://www.addgene.org/119723

Species: Synthetic
Genetic Insert: CaMPARI2
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4267; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30361563

Proper citation: RRID:Addgene_119723 Copy   

•   Source: Addgene
  

http://www.addgene.org/119720

Species: Homo sapiens
Genetic Insert: importinα
Vector Backbone Description: Backbone Marker:not commercially available one. Note that Eco47III, AgeI, BspE1 are missing; Vector Backbone:pmOrange-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22313767
Comments: pmOrange-impα was constructed by replacing the AgeI-BglII fragment encoding DsRed of pDsRed-imp α (Addgene plasmid 119679) with mOrange derived from pRSET-B-mOrange (provided by Professor Roger Y. Tsien, UCSD).

Proper citation: RRID:Addgene_119720 Copy   

•   Source: Addgene
  

http://www.addgene.org/119683

Species: Homo sapiens
Genetic Insert: OPTN
Vector Backbone Description: Vector Backbone:pBMN; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30679426

Proper citation: RRID:Addgene_119683 Copy   

•   Source: Addgene
  

http://www.addgene.org/119692

Species: Homo sapiens
Genetic Insert: NBR1
Vector Backbone Description: Vector Backbone:pBMN; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30679426
Comments: Please Note: The Phenylalanine to Alanine mutation is at position 563, and the Tyrosine to Alanine mutation is at position 732 relative to the NCBI Reference Sequence NP_005890.2

Proper citation: RRID:Addgene_119692 Copy   

•   Source: Addgene
  

http://www.addgene.org/119693

Species: Homo sapiens
Genetic Insert: OPTN
Vector Backbone Description: Vector Backbone:pBMN; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30679426

Proper citation: RRID:Addgene_119693 Copy   

•   Source: Addgene
  

http://www.addgene.org/119691

Species: Homo sapiens
Genetic Insert: NBR1
Vector Backbone Description: Vector Backbone:pBMN; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30679426

Proper citation: RRID:Addgene_119691 Copy   

•   Source: Addgene
  

http://www.addgene.org/119710

Species: Cochliobolus heterostrophus (Pgpda), Herpes simplex virus (HSVtk), Neurospora crassa (Ttub)
Genetic Insert: Negative selection marker HSVtk (Pgpda:HSVtk:Ttub)
Vector Backbone Description: Backbone Marker:Diego Orzaez; Backbone Size:2690; Vector Backbone:pUPD2 (Addgene #68161); Vector Types:Synthetic Biology, Domestication of DNA parts for GoldenBraid asembly; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:29680684

Proper citation: RRID:Addgene_119710 Copy   

•   Source: Addgene
  

http://www.addgene.org/119719

Species: Homo sapiens
Genetic Insert: importinα
Vector Backbone Description: Backbone Marker:not commercially available one. Note that Eco47III, AgeI, BspE1 are missing.; Vector Backbone:pmCherry-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22313767
Comments: pmCherry-impα was constructed by replacing the AgeI-BglII fragment encoding DsRed of pDsRed-imp α (Addgene plasmid 119679) with mCherry derived from pRSET-B-mCherry (provided by Professor Roger Y. Tsien, UCSD).

Proper citation: RRID:Addgene_119719 Copy   

•   Source: Addgene
  

http://www.addgene.org/119715

Genetic Insert: PgpdA::YFP::TtrpC(←)-PtrpC:nptII::Ttub (→)
Vector Backbone Description: Backbone Marker:Diego Orzaez; Backbone Size:7295; Vector Backbone:pDGB3omega1; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:29680684

Proper citation: RRID:Addgene_119715 Copy   

•   Source: Addgene
  

http://www.addgene.org/119714

Species: Penicillium chrysogenum
Genetic Insert: Terminator paf
Vector Backbone Description: Backbone Marker:Diego Orzaez; Backbone Size:2690; Vector Backbone:pUPD2 (Addgene #68161); Vector Types:Synthetic Biology, Domestication of DNA parts for GoldenBraid asembly; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:29680684

Proper citation: RRID:Addgene_119714 Copy   

•   Source: Addgene
  

http://www.addgene.org/119681

Species: Homo sapiens
Genetic Insert: OPTN
Vector Backbone Description: Vector Backbone:pBMN; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30679426

Proper citation: RRID:Addgene_119681 Copy   

•   Source: Addgene
  

http://www.addgene.org/119827

Species: Mus musculus
Genetic Insert: Type I procollagen α2 chain
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30287488
Comments: These plasmids were constructed after the G610C mouse model of OI in which the Gly610 residue (starting from the N-terminal end of the triple helix) was substituted with Cys. These mouse type I procollagen plasmids were designed for immortalized and primary murine osteoblasts and fibroblasts. Their expression in other cells might cause significant disruption of assembly, folding and trafficking of type I procollagen, resulting in artifacts. Even in osteoblasts and fibroblasts a fraction of transfected chains, particularly proα2, might be trafficked/degraded as monomers instead of being integrated into procollagen heterotrimers. In our experience, the best evidence of normal behavior of the transfected chains is the appearance of fluorescent extracellular collagen fibers ~ 12 h after transfection. Fluorescent molecules should be incorporated into fibers even when the transfected chains contain the N-propeptide cleavage site, because the cleavage is highly conformation sensitive and always incomplete. We recommend following these guidelines when using the plasmids 1) Only mouse cells that have high expression of endogenous type I procollagen are suitable for studies of physiologically-relevant processes with these constructs. 2) At least 100 μM ascorbic acid at and after transfection is required to ensure proper procollagen folding (for some cells, preincubation with ascorbic acid before transfection might be needed). 3) Optimization of transfection efficiency is needed for all cell types. 4) Experiments beyond 24h after transfection are not recommended, to avoid excessive accumulation of aggregates and cell malfunction caused by increased procollagen synthesis. 5) Lack of extracellular fluorescent fibers 12-24 h after transfection indicates improper procollagen synthesis/trafficking or cellular malfunction. 6) Excessive expression of transfected chains might cause rapid accumulation of large procollagen aggregates in the ER, resulting in cellular malfunction and death. Contact information: Shakib Omari ([email protected]), Sergey Leikin ([email protected]).

Proper citation: RRID:Addgene_119827 Copy   

•   Source: Addgene
  

http://www.addgene.org/119810

Genetic Insert: ecTadA-ecTadA*(7.10)-nSpCas9 (CP1300)
Vector Backbone Description: Vector Backbone:pCMV with a BR322 origin; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31110355

Proper citation: RRID:Addgene_119810 Copy   

•   Source: Addgene
  

http://www.addgene.org/119892

Genetic Insert: pLPD03031_GFP+
Vector Backbone Description: Vector Backbone:pAL5000/pMB1 shuttle vector; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28745867

Proper citation: RRID:Addgene_119892 Copy   

•   Source: Addgene
  

http://www.addgene.org/119893

Vector Backbone Description: Vector Backbone:pNG2; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:29366319

Proper citation: RRID:Addgene_119893 Copy   

•   Source: Addgene
  

http://www.addgene.org/119816

Species: Photinus pyralis
Genetic Insert: Luciferase
Vector Backbone Description: Backbone Size:8116; Vector Backbone:pLentipuro3/TO/V5-DEST; Vector Types:Mammalian Expression, Lentiviral, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30683938

Proper citation: RRID:Addgene_119816 Copy   

•   Source: Addgene