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Species: Aedes aegypti
Genetic Insert: LOC5576189 CCHamide receptor
Vector Backbone Description: Vector Backbone:pME18s; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30735632
Comments: The neuropeptide CDS was inserted into the pME18s plasmid using NotI and XhoI sites.
Proper citation: RRID:Addgene_115380 Copy
Species: Aedes aegypti
Genetic Insert: AAEL006076 AstAR
Vector Backbone Description: Vector Backbone:pME18s; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30735632
Comments: The neuropeptide CDS was inserted into the pME18s plasmid using NotI and XhoI sites.
Proper citation: RRID:Addgene_115381 Copy
Genetic Insert: Renilla Luciferase
Vector Backbone Description: Backbone Marker:ThermoFisher; Vector Backbone:pTK; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15337849
Comments: clone number 122. BoxB repeat sequence: gggccctgaagaagggccc
Proper citation: RRID:Addgene_115365 Copy
Genetic Insert: Renilla Luciferase
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5472; Vector Backbone:pCIneo; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16081698
Comments: clone number 137
Proper citation: RRID:Addgene_115366 Copy
Genetic Insert: Renilla Luciferase
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5472; Vector Backbone:pCIneo; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16081698
Comments: clone number 138
Proper citation: RRID:Addgene_115367 Copy
Genetic Insert: HA
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5472; Vector Backbone:pCIneo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15337849
Comments: clone number 211
Proper citation: RRID:Addgene_115360 Copy
Species: Homo sapiens
Genetic Insert: Argonaute 2
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5472; Vector Backbone:pCIneo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15337849
Comments: clone number 77. N-peptide = MDAQTRRRERRAEKQAQWKAAN
Proper citation: RRID:Addgene_115361 Copy
Species: Aedes aegypti
Genetic Insert: AAEL003747
Vector Backbone Description: Vector Backbone:pME18s; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30735632
Comments: The neuropeptide CDS was inserted into the pME18s plasmid using NotI and XhoI sites.
Proper citation: RRID:Addgene_115405 Copy
Species: Aedes aegypti
Genetic Insert: AAEL011475 CRZ R
Vector Backbone Description: Vector Backbone:pME18s; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30735632
Comments: The neuropeptide CDS was inserted into the pME18s plasmid using NotI and XhoI sites.
Note: Plasmid contains R27L and Q136R mutations in AAEL011475
Proper citation: RRID:Addgene_115406 Copy
Species: Aedes aegypti
Genetic Insert: AAEL008287 DH44 R
Vector Backbone Description: Vector Backbone:pME18s; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30735632
Comments: The neuropeptide CDS was inserted into the pME18s plasmid using NotI and XhoI sites.
Proper citation: RRID:Addgene_115407 Copy
Species: Aedes aegypti
Genetic Insert: AAEL012356 AstC R
Vector Backbone Description: Vector Backbone:pME18s; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30735632
Comments: The neuropeptide CDS was inserted into the pME18s plasmid using NotI and XhoI sites.
Proper citation: RRID:Addgene_115401 Copy
Genetic Insert: Renilla Luciferase
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5472; Vector Backbone:pCIneo; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16081698
Comments: clone number 143
Proper citation: RRID:Addgene_115369 Copy
Species: Aedes aegypti
Genetic Insert: ovo isoform X1
Vector Backbone Description: Vector Backbone:pME18s; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30735632
Comments: The neuropeptide CDS was inserted into the pME18s plasmid using NotI and XhoI sites.
Proper citation: RRID:Addgene_115402 Copy
Species: Aedes aegypti
Genetic Insert: AAEL017313 GPCR
Vector Backbone Description: Vector Backbone:pME18s; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30735632
Comments: The neuropeptide CDS was inserted into the pME18s plasmid using NotI and XhoI sites.
Proper citation: RRID:Addgene_115404 Copy
Species: Aedes aegypti
Genetic Insert: AAEL017335 CAPA R
Vector Backbone Description: Vector Backbone:pME18s; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30735632
Comments: The neuropeptide CDS was inserted into the pME18s plasmid using NotI and XhoI sites.
Proper citation: RRID:Addgene_115409 Copy
Species: A. aeolicus
Genetic Insert: tRNA methyltransferase
Vector Backbone Description: Backbone Size:6520; Vector Backbone:pHM6g; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:14517984
Comments: Please note that the plasmid pHM6g.AQ_1489 contains a Kanamycin resistance marker, and the BL21 bacterial strain contains a plasmid, pSJS1244, that confers spectinomycin resistance and allows for expression of genes with rare codons.
Addgene provides this plasmid in the DH5alpha bacterial strain. For expression, please use BL21(DE3).Star/pSJS1244
Proper citation: RRID:Addgene_11539 Copy
Species: M. jannaschii
Genetic Insert: phosphodiesterase
Vector Backbone Description: Backbone Size:6143; Vector Backbone:pLIC.B2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15128743
Comments: Please note that the plasmid pLIC.B2.MJ0936 contains an Ampicillin resistance marker, and the BL21 bacterial strain contains a plasmid, pSJS1244, that confers spectinomycin resistance and allows for expression of genes with rare codons.
Addgene provides this plasmid in the DH5alpha bacterial strain. For expression, please use BL21(DE3).Star/pSJS1244
Proper citation: RRID:Addgene_11538 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: HSP104
Vector Backbone Description: Backbone Size:4895; Vector Backbone:pRS316; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14978213
Proper citation: RRID:Addgene_1154 Copy
Species: bacteriophage P1
Genetic Insert: Phosphoglycerate kinase 1 promoter
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:2906; Vector Backbone:XhopKS; Vector Types:Mammalian Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Comments: Full Gene Name: PGK-Cre-bpA: Phosphoglycerate kinase I promoter (PGK), the bacteriophage P1 recombinase Cre with a SV40 large T antigen nuclear localization signal (NLS-Cre), bovine growth hormone polyadenylation site (bpA). Use to express the Cre recombinase in mammalian cells. The vector backbone is a variant of pBluescriptKS- (Stratagene), in which the Xbal site was converted to an Xhol site (provided by Paul Hasty) (Soriano P., Cell 1997). The plasmid was generated by Kurt Fellenberg. He cloned a NLS-Cre sequence, as published by Hua Gu (Gu H, Zou YR, Rajewsky K. 1993. Independent control of immunoglobulin switch recombination at individual switch regions evidenced through Cre-loxP-mediated gene targeting. Cell. 73:1155-1164) and cloned it into the pPGK-neo-bpA (Soriano P, Montgomery C, Geske R, Bradley A. 1991 Targeted disruption of the c-src proto-oncogene leads to osteopetrosis in mice. Cell. Feb 22;64(4):693-702.), replacing the neomycin resistance gene (neo) with the NLS-Cre.
NOTE: There are some discrepancies between the Addgene quality control sequence and the sequence provided by the depositing lab, but these differences should not affect plasmid function.
Proper citation: RRID:Addgene_11543 Copy
Species: Homo sapiens
Genetic Insert: GNB2 5'-UTR
Vector Backbone Description: Vector Backbone:pFL-SV40; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30373933
Comments: Note: Plasmid contains a GCC deletion and a T to C change in the GNB2 5'UTR. These changes are not known to affect plasmid function.
Proper citation: RRID:Addgene_115355 Copy
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