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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Authority Record Last Update Mentions Count
pGEX-αTAT1[2-236]
 
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1+ mentions
RRID:Addgene_27101 αTubulin K40 acetyltransferase Homo sapiens Ampicillin PMID:21068373 Backbone Size:4981; Vector Backbone:pGEX-6P1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin Addgene 2026-10-17 02:44:09 1
PTRF/Cavin1_R (OZ514)
 
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RRID:Addgene_27189 Zinc finger array targeting PTRF/Cavin1 Danio rerio Kanamycin PMID:18657511 This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene PTRF/Cavin1 (see below). Please note that this plasmid does NOT contain the PTRF/Cavin1 sequence. Users must order the complementary plasmid PTRF/Cavin1_L (OZ513) [Addgene plasmid 27188] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene. Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290 (Addgene plasmid 21872), pMLM292 (Addgene plasmid 21873), pMLM800 (Addgene plasmid 27202) and pMLM802 (Addgene plasmid 27203). This zinc finger array was tested for binding activity to the sequence 5'-GGAGGTGCC-3' in a bacterial two hybrid assay, and resulted in 6.81 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus). Scientists using this zinc finger array in a publication should notify [email protected] and acknowledge NIH grant number R01 GM088040 in the publication. Other Articles: "Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4(10):1471-1501. Pubmed ID: 19798082 "Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934 Backbone Size:5493; Vector Backbone:MG414; Vector Types:Zebrafish Targeting; Bacterial Resistance:Kanamycin Addgene 2026-10-17 02:44:10 0
zgc:153086_R (OZ512)
 
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RRID:Addgene_27187 Zinc finger array targeting zgc:153086 Danio rerio Kanamycin PMID:18657511 This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene zgc:153086 (see below). Please note that this plasmid does NOT contain the zgc:153086 sequence. Users must order the complementary plasmid zgc:153086_L (OZ511) [Addgene plasmid 27186] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene. Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290 (Addgene plasmid 21872), pMLM292 (Addgene plasmid 21873), pMLM800 (Addgene plasmid 27202) and pMLM802 (Addgene plasmid 27203). This zinc finger array was tested for binding activity to the sequence 5'-GGAGGTGAG-3' in a bacterial two hybrid assay, and resulted in 10.23 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus). Scientists using this zinc finger array in a publication should notify [email protected] and acknowledge NIH grant number R01 GM088040 in the publication. Other Articles: "Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4(10):1471-1501. Pubmed ID: 19798082 "Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934 Backbone Size:5493; Vector Backbone:MG414; Vector Types:Zebrafish Targeting; Bacterial Resistance:Kanamycin Addgene 2026-10-17 02:44:10 0
pGMEH-P38-luxAFB-DAS+4
 
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RRID:Addgene_27064 P#38-luxA-FLAG-luxB-DAS+4 synthetic gene Hygromycin PMID:21075796 Backbone Size:7843; Vector Backbone:pDE43-MEH; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin Addgene 2026-10-17 02:44:09 0
pLKO.1-MKL1/2 shRNA
 
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1+ mentions
RRID:Addgene_27161 shRNA MKL1+2 Mus musculus Ampicillin PMID:20466732 shRNA sequence for knockdown of both MKL1 and MKL2: CATGGAGCTGGTGGAGAAGAA Backbone Size:0; Vector Backbone:pLKO.1-puro; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin Addgene 2026-10-17 02:44:10 5
pMSG360
 
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RRID:Addgene_27155 Hygromycin PMID:20472794 Note that Addgene's sequencing results differ from the depositor's sequence at bp# 561, 565 and 829. Depositing scientist states that these differences do not affect the function of the plasmid. Backbone Marker:Glickman Lab; Backbone Size:4152; Vector Backbone:pmsg360; Vector Types:mycobacterial ko vector; Bacterial Resistance:Hygromycin Addgene 2026-10-17 02:44:09 0
pCGN-ELK-1
 
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1+ mentions
RRID:Addgene_27156 ELK1 Homo sapiens Ampicillin PMID:20466732 Backbone Size:0; Vector Backbone:pCGN; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin Addgene 2026-10-17 02:44:09 6
pBS-slpGFP
 
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RRID:Addgene_27171 surface (S)-layer protein promoter region fused with modified green fluorescent protein 5 Lactococcus lactis and synthetic construct Ampicillin PMID:20455948 Backbone Size:2959; Vector Backbone:pBluescript II KS(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin Addgene 2026-10-17 02:44:10 0
pCMV-BiP-Myc-KDEL P495L
 
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1+ mentions
RRID:Addgene_27166 BiP Homo sapiens Ampicillin PMID:12110171 Backbone Size:0; Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin Peptide Binding Mutant: P495L Addgene 2026-10-17 02:44:10 1
pRL-SV40P
 
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50+ mentions
RRID:Addgene_27163 SV40 promoter Ampicillin PMID:10373518 pRL-SV40P was generated by placing the simian virus 40 (SV40) promoter from pGL3-Promoter (Promega) into pRL-TK such that the SV40 promoter is driving the Renilla luciferase gene. Backbone Marker:Promega; Backbone Size:4045; Vector Backbone:pRL-TK; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin Addgene 2026-10-17 02:44:10 84
pcDNA-FLAG-Nv-NF-kB-Cys
 
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RRID:Addgene_27260 Nv-NF-kB Nematostella vectensis Ampicillin PMID:19806194 Clone starts at aa 2. Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin "Cys allele" = Cysteine at aa 67 Addgene 2026-10-17 02:44:11 0
pcDNA-FLAG-Nv-NF-kB-Ser
 
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RRID:Addgene_27259 Nv-NF-kB Nematostella vectensis Ampicillin PMID:19806194 Clone starts at aa 2. Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin "Ser allele" = Serine at aa 67 Addgene 2026-10-17 02:44:11 0
pcDNA-v-Rel
 
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1+ mentions
RRID:Addgene_27256 v-Rel Reticuloendotheliosis virus Ampicillin Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin Addgene 2026-10-17 02:44:11 1
SG-3'REL
 
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RRID:Addgene_27257 REL Homo sapiens Ampicillin PMID:14534540 Backbone Size:3396; Vector Backbone:pSG424; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin Contains aa 278-587 of REL Addgene 2026-10-17 02:44:11 0
pcDNA-FLAG-Mo-Trip6
 
Resource Report
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1+ mentions
RRID:Addgene_27254 Trip6 Mus musculus Ampicillin PMID:10395914 Trip6 cDNA starts at aa 2. Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin Addgene 2026-10-17 02:44:11 1
pcDNA-FLAG-Hu-Trip6
 
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1+ mentions
RRID:Addgene_27255 Trip6 Homo sapiens Ampicillin PMID:10794523 Trip6 cDNA contains starts at aa 2. Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin Addgene 2026-10-17 02:44:11 1
pcDNA-FLAG-REL
 
Resource Report
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1+ mentions
RRID:Addgene_27253 REL Homo sapiens Ampicillin PMID:18695674 Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin Addgene 2026-10-17 02:44:11 7
pGL4.11 promPDE4D3 Ebox mut
 
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RRID:Addgene_27304 prom PDE4D3 Homo sapiens Ampicillin PMID:20952536 Backbone Size:4370; Vector Backbone:pGL4.11; Vector Types:Luciferase; Bacterial Resistance:Ampicillin Ebox CACATG changed to GAGATG Addgene 2026-10-17 02:44:11 0
pDEST/N1-hBIN1-GFP
 
Resource Report
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1+ mentions
RRID:Addgene_27305 bridging integrator 1 (BIN1), transcript variant 8 Homo sapiens Kanamycin PMID:20169111 Backbone Size:4700; Vector Backbone:pDEST/N1-GFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin Addgene 2026-10-17 02:44:11 2
GST-SEPT5 WT
 
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RRID:Addgene_27269 Septin 5 Homo sapiens Ampicillin PMID:18385322 Backbone Marker:GE Healthcare Life Sciences; Backbone Size:4985; Vector Backbone:pGEX6P-2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin Addgene 2026-10-17 02:44:11 0

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