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Species: Synthetic
Genetic Insert: Venus
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25871405
Comments: Type 3a parts are used for N-terminal coding sequences. This plasmid encodes for an N-terminal fluorescent protein tag. For more information, please visit the supplemental information in PMID:25871405.
Proper citation: RRID:Addgene_65145 Copy
Species: Synthetic
Genetic Insert: mTurquoise2
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25871405
Comments: Type 3b parts are used for coding sequences. This plasmid encodes for a fluorescent protein. For more information, please visit the supplemental information in PMID:25871405.
Proper citation: RRID:Addgene_65151 Copy
Species: Synthetic
Genetic Insert: sgRNA Dropout
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25871405
Comments: Type 234 parts substitute a concatenation of part types 2, 3 and 4. For more information, please visit the supplemental information in PMID:25871405.
Proper citation: RRID:Addgene_65157 Copy
Species: Synthetic
Genetic Insert: GFP dropout
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25871405
Comments: Type 234r is used for the construction of dropout vectors. This plasmid encodes for a fluorescent dropout. For more information, please visit the supplemental information in PMID:25871405.
Proper citation: RRID:Addgene_65154 Copy
Species: Synthetic
Genetic Insert: mRuby2
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25871405
Comments: Type 3b parts are used for coding sequences. This plasmid encodes for a fluorescent protein. For more information, please visit the supplemental information in PMID:25871405.
Proper citation: RRID:Addgene_65153 Copy
Species: Synthetic
Genetic Insert: mTurquoise2
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25871405
Comments: Type 4a parts are used for C-terminal coding sequences. This plasmid encodes for a C-terminal fluorescent protein tag. For more information, please visit the supplemental information in PMID:25871405.
Proper citation: RRID:Addgene_65164 Copy
Species: Synthetic
Genetic Insert: 6XHis_3XFlag
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25871405
Comments: Type 4a parts are used for C-terminal coding sequences. This plasmid encodes for a C-terminal protein purification tag. For more information, please visit the supplemental information in PMID:25871405.
Proper citation: RRID:Addgene_65167 Copy
Species: Synthetic
Genetic Insert: ConR1
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25871405
Comments: Type 5 parts are 3' assembly connectors in the Duebery YTK. For more information, please visit the supplemental information in PMID:25871405.
Proper citation: RRID:Addgene_65174 Copy
Species: Synthetic
Genetic Insert: ConRE
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25871405
Comments: Type 5 parts are 3' assembly connectors in the Duebery YTK. For more information, please visit the supplemental information in PMID:25871405.
Proper citation: RRID:Addgene_65179 Copy
Species: Synthetic
Genetic Insert: ConR3
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25871405
Comments: Type 5 parts are 3' assembly connectors in the Duebery YTK. For more information, please visit the supplemental information in PMID:25871405.
Proper citation: RRID:Addgene_65176 Copy
Species: Synthetic
Genetic Insert: NourseothricinR
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25871405
Comments: Type 6 parts are Yeast Markers in the Dueber YTK. For more information, please visit the supplemental information in PMID:25871405.
Proper citation: RRID:Addgene_65185 Copy
Species: Synthetic
Genetic Insert: KanamycinR
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25871405
Comments: Type 6 parts are Yeast Markers in the Dueber YTK. For more information, please visit the supplemental information in PMID:25871405.
Proper citation: RRID:Addgene_65184 Copy
Species: Synthetic
Genetic Insert: ConRE'
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25871405
Comments: Type 5 parts are 3' assembly connectors in the Duebery YTK. For more information, please visit the supplemental information in PMID:25871405.
Proper citation: RRID:Addgene_65180 Copy
Species: Synthetic
Genetic Insert: ZeocinR
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25871405
Comments: Type 6 parts are Yeast Markers in the Dueber YTK. For more information, please visit the supplemental information in PMID:25871405.
Proper citation: RRID:Addgene_65187 Copy
Species: Synthetic
Genetic Insert: AmpR-ColE1
Vector Backbone Description: Vector Backbone:self-made; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25871405
Comments: Type 7 and 8 parts are Yeast propagation machinery in the Dueber YTK. For more information, please visit the supplemental information in PMID:25871405.
Proper citation: RRID:Addgene_65190 Copy
Species: Synthetic
Genetic Insert: SpecR-ColE1
Vector Backbone Description: Vector Backbone:self-made; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:25871405
Comments: Type 7 and 8 parts are Yeast propagation machinery in the Dueber YTK. For more information, please visit the supplemental information in PMID:25871405.
Proper citation: RRID:Addgene_65192 Copy
Species: Synthetic
Genetic Insert: KanR-ColE1
Vector Backbone Description: Vector Backbone:self-made; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25871405
Comments: Type 7 and 8 parts are Yeast propagation machinery in the Dueber YTK. For more information, please visit the supplemental information in PMID:25871405.
Proper citation: RRID:Addgene_65191 Copy
Species: Synthetic
Genetic Insert: GPI anchor
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pIRESneo3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22406856
Comments: luminal tagging
Proper citation: RRID:Addgene_65295 Copy
Species: Synthetic
Genetic Insert: GPI anchor
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pIRESneo3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22406856
Comments: luminal tagging
Proper citation: RRID:Addgene_65294 Copy
Species: Synthetic
Genetic Insert: URR1-RFP-Leu2-URR2
Vector Backbone Description: Backbone Marker:Agilent Technologies; Vector Backbone:pRS424; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25956650
Comments: pRS424 with URR1-RFP-Leu2-URR2 frament inserted at MCS, used for receiving in vitro ligated pathway by gap-repair method(URR1 and URR2 are homologous arms)
Proper citation: RRID:Addgene_65330 Copy
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