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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
pET28b – E. coli RecE residues 564-868
 
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RRID:Addgene_104534 RecE residues 564-866 (C-terminal nuclease domain) E. coli Kanamycin PMID:19446525 Backbone Marker:EMD Biosciences; Backbone Size:5368; Vector Backbone:pET28b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:00:43 0
pET-2(KW)2-mCherry
 
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RRID:Addgene_112738 2(KW)2-mCherry E. coli Ampicillin PMID:26264666 Note: The mCherry insert contains a N9Q mutation. The depositor has confirmed this does not affect plasmid function. Backbone Marker:Novagen; Backbone Size:5706; Vector Backbone:pET-15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:02:04 0
pET-2HMG-eGFP
 
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RRID:Addgene_112737 2HMG-eGFP E. coli Ampicillin Backbone Marker:Novagen; Backbone Size:5706; Vector Backbone:pET-15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:02:04 0
pPB-R1R2-EM7neoPheS
 
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1+ mentions
RRID:Addgene_112923 neoPheS E. coli Ampicillin PMID:30477585 Backbone Size:3470; Vector Backbone:pPB_LR5; Vector Types:Mammalian Expression, piggyBac transposon Gateway destination vector; Bacterial Resistance:Ampicillin 2026-08-15 01:02:06 1
pBLind
 
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RRID:Addgene_113109 RFP E. coli Kanamycin PMID:29596741 Vector Backbone:pBbE8K; Vector Types:Bacterial Expression, Cell-free; Bacterial Resistance:Kanamycin 2026-08-15 01:02:08 0
pAW218
 
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RRID:Addgene_113240 Cas1 E. coli Streptomycin PMID:28729350 Backbone Size:4000; Vector Backbone:pCDF1b; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin 2026-08-15 01:02:10 0
pAW204
 
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RRID:Addgene_113244 IHFa F13A E. coli Chloramphenicol PMID:28729350 Backbone Size:3600; Vector Backbone:pACYC; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol F13A 2026-08-15 01:02:10 0
pAW215
 
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RRID:Addgene_113237 Cas1 E. coli Streptomycin PMID:28729350 Backbone Size:4000; Vector Backbone:pCDF1b; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin 2026-08-15 01:02:10 0
pAW214
 
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RRID:Addgene_113236 Cas1 E. coli Streptomycin PMID:28729350 Backbone Size:4000; Vector Backbone:pCDF1b; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin 2026-08-15 01:02:10 0
pAW213
 
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RRID:Addgene_113235 Cas1 E. coli Streptomycin PMID:28729350 Backbone Size:4000; Vector Backbone:pCDF1b; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin 2026-08-15 01:02:10 0
pAW217
 
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RRID:Addgene_113239 Cas1 E. coli Streptomycin PMID:28729350 Backbone Size:4000; Vector Backbone:pCDF1b; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin 2026-08-15 01:02:10 0
pAW186
 
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RRID:Addgene_113184 Cas1 E. coli Streptomycin PMID:28729350 Backbone Size:4000; Vector Backbone:pCDF1b; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin R163A 2026-08-15 01:02:09 0
pAW151
 
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RRID:Addgene_113183 Cas1 E. coli Streptomycin PMID:28729350 Backbone Size:4000; Vector Backbone:pCDF1b; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin R146A 2026-08-15 01:02:09 0
pAW150
 
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RRID:Addgene_113182 Cas1 E. coli Streptomycin PMID:28729350 Backbone Size:4000; Vector Backbone:pCDF1b; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin S143A 2026-08-15 01:02:09 0
pAW209
 
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RRID:Addgene_113188 Cas1 E. coli Streptomycin PMID:28729350 Backbone Size:4000; Vector Backbone:pCDF1b; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin 2026-08-15 01:02:09 0
pAW167
 
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RRID:Addgene_113187 Cas1 E. coli Streptomycin PMID:28729350 Backbone Size:4000; Vector Backbone:pCDF1b; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin 2026-08-15 01:02:09 0
pAW184
 
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RRID:Addgene_113176 Cas1 E. coli Streptomycin PMID:28729350 Backbone Size:4000; Vector Backbone:pCDF1b; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin R112A 2026-08-15 01:02:09 0
pAW174
 
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RRID:Addgene_113181 Cas1 E. coli Streptomycin PMID:28729350 Backbone Size:4000; Vector Backbone:pCDF1b; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin R138A 2026-08-15 01:02:09 0
pCas-CmR(+)
 
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RRID:Addgene_113633 Chloramphenicol resistance gene E. coli Chloramphenicol PMID:29884781 Vector Backbone:pCas; Vector Types:CRISPR; Bacterial Resistance:Chloramphenicol 2026-08-15 01:02:13 0
aav-CAG-FLEX-tdNfsB(F124W)-mCherry (eNTR)
 
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RRID:Addgene_113762 tdNfsB(F124W)-mCherry E. coli Ampicillin PMID:30111097 Sternson Lab recommendations for propagation of AAV-FLEX plasmids: "These vectors are prone to recombination. This is a well known issue with these AAV vectors and is due to the inverted terminal repeats (ITRs) required for AAV production. To minimize recombination, we propagate these plasmids in NEB Stable cells. Also, to minimize recombination, cells should be cultured at 30 C. Note that these cultures will grow slowly (20 h for minipreps). Better yields and culture times are obtained with 2xYT as the media. This is strongly recommended. Because recombination may still happen occasionally, we do a panel of restriction digestions to assess whether the ITRs are in tact. Separate digestions with PvuII, Sma1, and SnaB1 should be performed." Backbone Marker:Scott Sternson; Vector Backbone:AAV2; Vector Types:Mammalian Expression, AAV, Cre/Lox; Bacterial Resistance:Ampicillin F124W 2026-08-15 01:02:15 0

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