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http://www.addgene.org/159881

Species: Caenorhabditis elegans
Genetic Insert: Intron GG2 - 900 bp
Vector Backbone Description: Vector Backbone:pDONR221; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33298957
Comments: See www.wormbuilder.org for more information.

Proper citation: RRID:Addgene_159881 Copy   

•   Source: Addgene

http://www.addgene.org/159882

Species: Caenorhabditis elegans
Genetic Insert: Intron GG3 - 900 bp
Vector Backbone Description: Vector Backbone:pDONR221; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33298957
Comments: See www.wormbuilder.org for more information.

Proper citation: RRID:Addgene_159882 Copy   

•   Source: Addgene

http://www.addgene.org/159896

Species: Caenorhabditis elegans
Genetic Insert: Pmlc-1 | mNeonGreen | cbr-tbb-2 UTR
Vector Backbone Description: Vector Backbone:pDEST R4-R3; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32908967
Comments: Synthetic Genome Biology lab @ KAUST www.wormbuilder.org

Proper citation: RRID:Addgene_159896 Copy   

•   Source: Addgene

http://www.addgene.org/65631

Species: Caenorhabditis elegans
Genetic Insert: Peft-3::gfp::h2b::tbb-2 3'UTR
Vector Backbone Description: Backbone Marker:Genscript; Backbone Size:2710; Vector Backbone:pUC57; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26044730
Comments: Please note: eft-3 has officially been changed to eef-1A.1 Please see the eef-1A.1 WormBase entry for details: http://www.wormbase.org/species/c_elegans/gene/WBGene00001168#05-9g-3

Proper citation: RRID:Addgene_65631 Copy   

•   Source: Addgene

http://www.addgene.org/65597

Species: Caenorhabditis elegans
Genetic Insert: sgRNA for APs12
Vector Backbone Description: Backbone Marker:Goldstein lab Plasmid 47549; Vector Backbone:pDD162; Vector Types:Worm Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25249454
Comments: From the depositor: Each positive clones had been sequenced for correct insertion of the sgRNA . However the PCR could have created some mutation in the plasmid backbone (containing also the Cas9 gene) and we didnt sequenced the full plasmid, therefore we recommend to use a mix of several positive clones. It is why we provide several clones containing the same sgRNA insert.

Proper citation: RRID:Addgene_65597 Copy   

•   Source: Addgene

http://www.addgene.org/65632

Species: Caenorhabditis elegans
Genetic Insert: Peft-3::gfp::h2b::tbb-2 3'UTR, C. briggsae unc-119 rescue fragment
Vector Backbone Description: Backbone Marker:Genscript; Backbone Size:2710; Vector Backbone:pUC57; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26044730
Comments: Please note: eft-3 has officially been changed to eef-1A.1 Please see the eef-1A.1 WormBase entry for details: http://www.wormbase.org/species/c_elegans/gene/WBGene00001168#05-9g-3

Proper citation: RRID:Addgene_65632 Copy   

•   Source: Addgene

http://www.addgene.org/65594

Species: Caenorhabditis elegans
Genetic Insert: sgRNA for APa13
Vector Backbone Description: Backbone Marker:Goldstein lab Plasmid 47549; Vector Backbone:pDD162; Vector Types:Worm Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25249454
Comments: From the depositor: Each positive clones had been sequenced for correct insertion of the sgRNA . However the PCR could have created some mutation in the plasmid backbone (containing also the Cas9 gene) and we didnt sequenced the full plasmid, therefore we recommend to use a mix of several positive clones. It is why we provide several clones containing the same sgRNA insert.

Proper citation: RRID:Addgene_65594 Copy   

•   Source: Addgene

http://www.addgene.org/65593

Species: Caenorhabditis elegans
Genetic Insert: sgRNA for APa13
Vector Backbone Description: Backbone Marker:Goldstein lab Plasmid 47549; Vector Backbone:pDD162; Vector Types:Worm Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25249454
Comments: From the depositor: Each positive clones had been sequenced for correct insertion of the sgRNA . However the PCR could have created some mutation in the plasmid backbone (containing also the Cas9 gene) and we didnt sequenced the full plasmid, therefore we recommend to use a mix of several positive clones. It is why we provide several clones containing the same sgRNA insert.

Proper citation: RRID:Addgene_65593 Copy   

•   Source: Addgene

http://www.addgene.org/65596

Species: Caenorhabditis elegans
Genetic Insert: sgRNA for APa13
Vector Backbone Description: Backbone Marker:Goldstein lab Plasmid 47549; Vector Backbone:pDD162; Vector Types:Worm Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25249454
Comments: From the depositor: Each positive clones had been sequenced for correct insertion of the sgRNA . However the PCR could have created some mutation in the plasmid backbone (containing also the Cas9 gene) and we didnt sequenced the full plasmid, therefore we recommend to use a mix of several positive clones. It is why we provide several clones containing the same sgRNA insert.

Proper citation: RRID:Addgene_65596 Copy   

•   Source: Addgene

http://www.addgene.org/65827

Species: Caenorhabditis elegans
Genetic Insert: NLG-1
Vector Backbone Description: Backbone Marker:C.Bargmann; Backbone Size:3500; Vector Backbone:pSM; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18255029

Proper citation: RRID:Addgene_65827 Copy   

•   Source: Addgene

http://www.addgene.org/66101

Species: Caenorhabditis elegans
Genetic Insert: Chrimson
Vector Backbone Description: Vector Backbone:pUC57-Kan; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26022242
Comments: This construct was produced through de novo gene synthesis. Chrimson amino acid sequence was published in: Klapoetke NC, Murata Y, Kim SS, Pulver SR, Birdsey-Benson A, Cho YK, Morimoto TK, Chuong AS, Carpenter EJ, Tian Z, Wang J, Xie Y, Yan Z, Zhang Y, Chow BY, Surek B, Melkonian M, Jayaraman V, Constantine-Paton M, Wong GK, and Boyden ES. 2014. Independent optical excitation of distinct neural populations. Nat. Meth. 11, 338-346.

Proper citation: RRID:Addgene_66101 Copy   

•   Source: Addgene

http://www.addgene.org/66102

Species: Caenorhabditis elegans
Genetic Insert: Chronos
Vector Backbone Description: Vector Backbone:pUC57-Kan; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26022242
Comments: This construct was produced through de novo gene synthesis. Chronos amino acid sequence was published in: Klapoetke NC, Murata Y, Kim SS, Pulver SR, Birdsey-Benson A, Cho YK, Morimoto TK, Chuong AS, Carpenter EJ, Tian Z, Wang J, Xie Y, Yan Z, Zhang Y, Chow BY, Surek B, Melkonian M, Jayaraman V, Constantine-Paton M, Wong GK, and Boyden ES. 2014. Independent optical excitation of distinct neural populations. Nat. Meth. 11, 338-346.

Proper citation: RRID:Addgene_66102 Copy   

•   Source: Addgene

http://www.addgene.org/66861

Species: Caenorhabditis elegans
Genetic Insert: Let-60
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pClneo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23103556

Proper citation: RRID:Addgene_66861 Copy   

•   Source: Addgene

http://www.addgene.org/66826

Species: Caenorhabditis elegans
Genetic Insert: mKate2-C1^SEC^3xFlag
Vector Backbone Description: Backbone Size:2600; Vector Backbone:pUC19 (modified); Vector Types:Worm Expression, Cre/Lox, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26044593
Comments: THE DEPOSITING LAB NO LONGER USES THIS CONSTRUCT AND RECOMMENDS pDD287 (https://www.addgene.org/70685/). The full sequence for this plasmid can still be found by clicking the link next to "Depositor Sequences". IMPORTANT NOTE: The repeat regions in this plasmid make it susceptible to recombination. The depositing lab grows the plasmid at 30°C. You may also want to prep and digest multiple single colonies to verify that you have a clone that has not recombined.

Proper citation: RRID:Addgene_66826 Copy   

•   Source: Addgene

http://www.addgene.org/66825

Species: Caenorhabditis elegans
Genetic Insert: TagRFP-T-C1^SEC^3xFlag
Vector Backbone Description: Backbone Size:2600; Vector Backbone:pUC19 (modified); Vector Types:Worm Expression, Cre/Lox, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26044593
Comments: THE DEPOSITING LAB NO LONGER USES THIS CONSTRUCT AND RECOMMENDS pDD286 (https://www.addgene.org/70684/). The full sequence for this plasmid can still be found by clicking the link next to "Addgene Sequences" and scrolling to the bottom of the page for "Full Addgene NGS sequence". IMPORTANT NOTE: The repeat regions in this plasmid make it susceptible to recombination. The depositing lab grows the plasmid at 30°C. You may also want to prep and digest multiple single colonies to verify that you have a clone that has not recombined.

Proper citation: RRID:Addgene_66825 Copy   

•   Source: Addgene

http://www.addgene.org/66859

Species: Caenorhabditis elegans
Genetic Insert: CST-1
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pClneo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23103556
Comments: Addgene sequencing results find amino acid changes S48N and H50R compared to available CST-1 reference sequences.

Proper citation: RRID:Addgene_66859 Copy   

•   Source: Addgene

http://www.addgene.org/66858

Species: Caenorhabditis elegans
Genetic Insert: YAP-1
Vector Backbone Description: Backbone Marker:Addgene; Vector Backbone:PD95.75; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23396260
Comments: I performed PCR on C.elegans genome to isolate the genome of YAP-1 which contain 5' UTR and the coding exons and introns. I subcloned the product into PstI/SmaI sites of PD95.75, so that GFP is fused to the last exon. Serine 104 was mutated to alanine.

Proper citation: RRID:Addgene_66858 Copy   

•   Source: Addgene

http://www.addgene.org/66854

Species: Caenorhabditis elegans
Genetic Insert: YAP-1
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pClneo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23396260
Comments: Addgene sequencing results find amino acid changes Q48R and Y213F compared to available YAP-1 reference sequences.

Proper citation: RRID:Addgene_66854 Copy   

•   Source: Addgene

http://www.addgene.org/66863

Species: Caenorhabditis elegans
Genetic Insert: RSF-1
Vector Backbone Description: Backbone Marker:Addgene; Vector Backbone:PD95.75; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23103556
Comments: I performed PCR on C.elegans genome to isolate the genome of RSF-1 which contain 5' UTR and the coding exons and introns. I subcloned the product into PstI/SmaI sites of PD95.75, so that GFP is fused to the last exon.

Proper citation: RRID:Addgene_66863 Copy   

•   Source: Addgene

http://www.addgene.org/84830

Species: Caenorhabditis elegans
Genetic Insert: C. elegans codon optimized tdTomato
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pDESTR4-R3; Vector Types:Bacterial Expression, Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27374334
Comments: Expression vector with C. elegans codon-optimized tdTomato under the eft-3 promoter and with the tbb-2 3'UTR. Cloned into pCFJ150 for compatibility with mosSCI. Contains cbr-unc-119 rescue fragment.

Proper citation: RRID:Addgene_84830 Copy   

•   Source: Addgene