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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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pCFJ2259 - Intron GG2 - 900 bp Resource Report Resource Website |
RRID:Addgene_159881 | Intron GG2 - 900 bp | Caenorhabditis elegans | Kanamycin | PMID:33298957 | See www.wormbuilder.org for more information. | Vector Backbone:pDONR221; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin | Not applicable | 2026-08-15 01:08:32 | 0 |
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pCFJ2215 - Intron GG3 - 900 bp Resource Report Resource Website |
RRID:Addgene_159882 | Intron GG3 - 900 bp | Caenorhabditis elegans | Kanamycin | PMID:33298957 | See www.wormbuilder.org for more information. | Vector Backbone:pDONR221; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin | Not applicable | 2026-08-15 01:08:35 | 0 |
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pSEM229 - [Pmlc-1 | mNeonGreen | cbr-tbb-2 UTR] Resource Report Resource Website |
RRID:Addgene_159896 | Pmlc-1 | mNeonGreen | cbr-tbb-2 UTR | Caenorhabditis elegans | Ampicillin | PMID:32908967 | Synthetic Genome Biology lab @ KAUST www.wormbuilder.org | Vector Backbone:pDEST R4-R3; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:08:33 | 0 | |
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pIK127 Resource Report Resource Website |
RRID:Addgene_65631 | Peft-3::gfp::h2b::tbb-2 3'UTR | Caenorhabditis elegans | Ampicillin | PMID:26044730 | Please note: eft-3 has officially been changed to eef-1A.1 Please see the eef-1A.1 WormBase entry for details: http://www.wormbase.org/species/c_elegans/gene/WBGene00001168#05-9g-3 | Backbone Marker:Genscript; Backbone Size:2710; Vector Backbone:pUC57; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:18:20 | 0 | |
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AP54-3 Resource Report Resource Website |
RRID:Addgene_65597 | sgRNA for APs12 | Caenorhabditis elegans | Ampicillin | PMID:25249454 | From the depositor: Each positive clones had been sequenced for correct insertion of the sgRNA . However the PCR could have created some mutation in the plasmid backbone (containing also the Cas9 gene) and we didnt sequenced the full plasmid, therefore we recommend to use a mix of several positive clones. It is why we provide several clones containing the same sgRNA insert. | Backbone Marker:Goldstein lab Plasmid 47549; Vector Backbone:pDD162; Vector Types:Worm Expression, CRISPR; Bacterial Resistance:Ampicillin | 2026-08-15 01:18:20 | 0 | |
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pIK137 Resource Report Resource Website |
RRID:Addgene_65632 | Peft-3::gfp::h2b::tbb-2 3'UTR, C. briggsae unc-119 rescue fragment | Caenorhabditis elegans | Ampicillin | PMID:26044730 | Please note: eft-3 has officially been changed to eef-1A.1 Please see the eef-1A.1 WormBase entry for details: http://www.wormbase.org/species/c_elegans/gene/WBGene00001168#05-9g-3 | Backbone Marker:Genscript; Backbone Size:2710; Vector Backbone:pUC57; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:18:21 | 0 | |
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AP56-7 Resource Report Resource Website |
RRID:Addgene_65594 | sgRNA for APa13 | Caenorhabditis elegans | Ampicillin | PMID:25249454 | From the depositor: Each positive clones had been sequenced for correct insertion of the sgRNA . However the PCR could have created some mutation in the plasmid backbone (containing also the Cas9 gene) and we didnt sequenced the full plasmid, therefore we recommend to use a mix of several positive clones. It is why we provide several clones containing the same sgRNA insert. | Backbone Marker:Goldstein lab Plasmid 47549; Vector Backbone:pDD162; Vector Types:Worm Expression, CRISPR; Bacterial Resistance:Ampicillin | 2026-08-15 01:18:19 | 0 | |
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AP56-6 Resource Report Resource Website |
RRID:Addgene_65593 | sgRNA for APa13 | Caenorhabditis elegans | Ampicillin | PMID:25249454 | From the depositor: Each positive clones had been sequenced for correct insertion of the sgRNA . However the PCR could have created some mutation in the plasmid backbone (containing also the Cas9 gene) and we didnt sequenced the full plasmid, therefore we recommend to use a mix of several positive clones. It is why we provide several clones containing the same sgRNA insert. | Backbone Marker:Goldstein lab Plasmid 47549; Vector Backbone:pDD162; Vector Types:Worm Expression, CRISPR; Bacterial Resistance:Ampicillin | 2026-08-15 01:18:19 | 0 | |
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AP56-8 Resource Report Resource Website |
RRID:Addgene_65596 | sgRNA for APa13 | Caenorhabditis elegans | Ampicillin | PMID:25249454 | From the depositor: Each positive clones had been sequenced for correct insertion of the sgRNA . However the PCR could have created some mutation in the plasmid backbone (containing also the Cas9 gene) and we didnt sequenced the full plasmid, therefore we recommend to use a mix of several positive clones. It is why we provide several clones containing the same sgRNA insert. | Backbone Marker:Goldstein lab Plasmid 47549; Vector Backbone:pDD162; Vector Types:Worm Expression, CRISPR; Bacterial Resistance:Ampicillin | 2026-08-15 01:18:19 | 0 | |
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Punc-4::NGL-1::spGFP1-10 Resource Report Resource Website 1+ mentions |
RRID:Addgene_65827 | NLG-1 | Caenorhabditis elegans | Ampicillin | PMID:18255029 | Backbone Marker:C.Bargmann; Backbone Size:3500; Vector Backbone:pSM; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:18:23 | 1 | ||
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dg263_pENTR_ChrimsonCel Resource Report Resource Website 1+ mentions |
RRID:Addgene_66101 | Chrimson | Caenorhabditis elegans | Kanamycin | PMID:26022242 | This construct was produced through de novo gene synthesis. Chrimson amino acid sequence was published in: Klapoetke NC, Murata Y, Kim SS, Pulver SR, Birdsey-Benson A, Cho YK, Morimoto TK, Chuong AS, Carpenter EJ, Tian Z, Wang J, Xie Y, Yan Z, Zhang Y, Chow BY, Surek B, Melkonian M, Jayaraman V, Constantine-Paton M, Wong GK, and Boyden ES. 2014. Independent optical excitation of distinct neural populations. Nat. Meth. 11, 338-346. | Vector Backbone:pUC57-Kan; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin | 2026-08-15 01:18:24 | 1 | |
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dg264_pENTR_ChronosCel Resource Report Resource Website |
RRID:Addgene_66102 | Chronos | Caenorhabditis elegans | Kanamycin | PMID:26022242 | This construct was produced through de novo gene synthesis. Chronos amino acid sequence was published in: Klapoetke NC, Murata Y, Kim SS, Pulver SR, Birdsey-Benson A, Cho YK, Morimoto TK, Chuong AS, Carpenter EJ, Tian Z, Wang J, Xie Y, Yan Z, Zhang Y, Chow BY, Surek B, Melkonian M, Jayaraman V, Constantine-Paton M, Wong GK, and Boyden ES. 2014. Independent optical excitation of distinct neural populations. Nat. Meth. 11, 338-346. | Vector Backbone:pUC57-Kan; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin | 2026-08-15 01:18:24 | 0 | |
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pClneoEGFPC-let60 K16N Resource Report Resource Website |
RRID:Addgene_66861 | Let-60 | Caenorhabditis elegans | Ampicillin | PMID:23103556 | Backbone Marker:Promega; Vector Backbone:pClneo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | Lysine 16 is mutated to asparagine | 2026-08-15 01:18:30 | 0 | |
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pDD285 Resource Report Resource Website |
RRID:Addgene_66826 | mKate2-C1^SEC^3xFlag | Caenorhabditis elegans | Ampicillin | PMID:26044593 | THE DEPOSITING LAB NO LONGER USES THIS CONSTRUCT AND RECOMMENDS pDD287 (https://www.addgene.org/70685/). The full sequence for this plasmid can still be found by clicking the link next to "Depositor Sequences". IMPORTANT NOTE: The repeat regions in this plasmid make it susceptible to recombination. The depositing lab grows the plasmid at 30°C. You may also want to prep and digest multiple single colonies to verify that you have a clone that has not recombined. | Backbone Size:2600; Vector Backbone:pUC19 (modified); Vector Types:Worm Expression, Cre/Lox, CRISPR; Bacterial Resistance:Ampicillin | 2026-08-15 01:18:32 | 0 | |
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pDD284 Resource Report Resource Website 1+ mentions |
RRID:Addgene_66825 | TagRFP-T-C1^SEC^3xFlag | Caenorhabditis elegans | Ampicillin | PMID:26044593 | THE DEPOSITING LAB NO LONGER USES THIS CONSTRUCT AND RECOMMENDS pDD286 (https://www.addgene.org/70684/). The full sequence for this plasmid can still be found by clicking the link next to "Addgene Sequences" and scrolling to the bottom of the page for "Full Addgene NGS sequence". IMPORTANT NOTE: The repeat regions in this plasmid make it susceptible to recombination. The depositing lab grows the plasmid at 30°C. You may also want to prep and digest multiple single colonies to verify that you have a clone that has not recombined. | Backbone Size:2600; Vector Backbone:pUC19 (modified); Vector Types:Worm Expression, Cre/Lox, CRISPR; Bacterial Resistance:Ampicillin | 2026-08-15 01:18:30 | 5 | |
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pClneoMyc' CST-1 Resource Report Resource Website 1+ mentions |
RRID:Addgene_66859 | CST-1 | Caenorhabditis elegans | Ampicillin | PMID:23103556 | Addgene sequencing results find amino acid changes S48N and H50R compared to available CST-1 reference sequences. | Backbone Marker:Promega; Vector Backbone:pClneo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | S48N, H50R, S257P and V340A mutations in CST-1 compared to GenBank reference sequence NP_001024575.2. CST-1 ends at amino acid 444 | 2026-08-15 01:18:30 | 1 |
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pPD95.75-cYAP-1 S104A Resource Report Resource Website |
RRID:Addgene_66858 | YAP-1 | Caenorhabditis elegans | Ampicillin | PMID:23396260 | I performed PCR on C.elegans genome to isolate the genome of YAP-1 which contain 5' UTR and the coding exons and introns. I subcloned the product into PstI/SmaI sites of PD95.75, so that GFP is fused to the last exon. Serine 104 was mutated to alanine. | Backbone Marker:Addgene; Vector Backbone:PD95.75; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin | Serine104 is mutated to alanine | 2026-08-15 01:18:30 | 0 |
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pClneoMyc'-cYAP-1 Resource Report Resource Website |
RRID:Addgene_66854 | YAP-1 | Caenorhabditis elegans | Ampicillin | PMID:23396260 | Addgene sequencing results find amino acid changes Q48R and Y213F compared to available YAP-1 reference sequences. | Backbone Marker:Promega; Vector Backbone:pClneo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:18:32 | 0 | |
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pPD95.75 RSF-1 Resource Report Resource Website |
RRID:Addgene_66863 | RSF-1 | Caenorhabditis elegans | Ampicillin | PMID:23103556 | I performed PCR on C.elegans genome to isolate the genome of RSF-1 which contain 5' UTR and the coding exons and introns. I subcloned the product into PstI/SmaI sites of PD95.75, so that GFP is fused to the last exon. | Backbone Marker:Addgene; Vector Backbone:PD95.75; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:18:33 | 0 | |
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pCFJ1955 Resource Report Resource Website 1+ mentions |
RRID:Addgene_84830 | C. elegans codon optimized tdTomato | Caenorhabditis elegans | Ampicillin | PMID:27374334 | Expression vector with C. elegans codon-optimized tdTomato under the eft-3 promoter and with the tbb-2 3'UTR. Cloned into pCFJ150 for compatibility with mosSCI. Contains cbr-unc-119 rescue fragment. | Backbone Marker:Invitrogen; Vector Backbone:pDESTR4-R3; Vector Types:Bacterial Expression, Worm Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:21:19 | 1 |
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