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Species: Arabidopsis thaliana
Genetic Insert: promotor RPS5a (At3g11940; 1700bp)
Vector Backbone Description: Vector Backbone:pICH41295 (Engler et al., 2014); Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:29847550
Proper citation: RRID:Addgene_105356 Copy
Species: Arabidopsis thaliana
Genetic Insert: promotor UBQ10 (At4g05320; 800bp)
Vector Backbone Description: Vector Backbone:pICH41295 (Engler et al., 2014); Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:29847550
Proper citation: RRID:Addgene_105355 Copy
Species: Arabidopsis thaliana
Genetic Insert: promotor PR1 (At2g14610; 1200bp)
Vector Backbone Description: Vector Backbone:pICH41295 (Engler et al., 2014); Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:29847550
Proper citation: RRID:Addgene_105370 Copy
Species: Arabidopsis thaliana
Genetic Insert: promotor SESA3/ AtS2S3 (At4g27160; 550bp)
Vector Backbone Description: Vector Backbone:pICH41295 (Engler et al., 2014); Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:29847550
Proper citation: RRID:Addgene_105371 Copy
Species: Arabidopsis thaliana
Genetic Insert: promotor PAD4 (At3g52430)
Vector Backbone Description: Vector Backbone:pAGM1251 (Engler et al., 2014); Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:29847550
Proper citation: RRID:Addgene_105367 Copy
Species: Arabidopsis thaliana
Genetic Insert: promotor EDS1 (At3g48090)
Vector Backbone Description: Vector Backbone:pAGM1251 (Engler et al., 2014); Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:29847550
Proper citation: RRID:Addgene_105365 Copy
Species: Arabidopsis thaliana
Genetic Insert: promotor (At1g66850;350bp)
Vector Backbone Description: Vector Backbone:pICH41295 (Engler et al., 2014); Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:29847550
Proper citation: RRID:Addgene_105363 Copy
Species: Arabidopsis thaliana
Genetic Insert: promotor ICU2 (At5g67100;600bp)
Vector Backbone Description: Vector Backbone:pICH41295 (Engler et al., 2014); Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:29847550
Proper citation: RRID:Addgene_105361 Copy
Species: Arabidopsis thaliana
Genetic Insert: promotor GILT (At4g12960;2100bp)
Vector Backbone Description: Vector Backbone:pICH41295 (Engler et al., 2014); Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:29847550
Proper citation: RRID:Addgene_105362 Copy
Species: Arabidopsis thaliana
Genetic Insert: U6-26p::sgRNA scaffold
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:3017; Vector Backbone:pGEM T-easy; Vector Types:Plant Expression, CRISPR, Synthetic Biology, Subcloning; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34541122
Comments: The new version of pFH6 (pFH6_new) provides > 10 times higher mutation efficiencies than the original version. In combination with pUB-Cas9, homozygous knockout plants can be created in Arabidopsis in the T2 generation at efficiencies of 50-100%.
For cloning procedure, please use the attached protocol which is derived from Hahn et al., 2017 ( http://www.bio-protocol.org/e2384 ).
Proper citation: RRID:Addgene_105866 Copy
Species: Arabidopsis thaliana
Genetic Insert: pAtU6
Vector Backbone Description: Vector Backbone:pGEM-T Easy; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34717688
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.05.20.444986v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_167665 Copy
Species: Arabidopsis thaliana
Genetic Insert: pAtU3
Vector Backbone Description: Vector Backbone:pGEM-T Easy; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34717688
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.05.20.444986v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_167664 Copy
Species: Arabidopsis thaliana
Genetic Insert: pAtU6
Vector Backbone Description: Vector Backbone:pGEM-T Easy; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34717688
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.05.20.444986v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_167663 Copy
Species: Arabidopsis thaliana
Genetic Insert: auxin-inducible degron (aid)
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_169360 Copy
Species: Arabidopsis thaliana
Genetic Insert: auxin-inducible degron (aid)
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_169361 Copy
Species: Arabidopsis thaliana
Genetic Insert: auxin-inducible degron (aid)
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_169356 Copy
Species: Arabidopsis thaliana
Genetic Insert: auxin-inducible degron (aid)
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_169357 Copy
Species: Arabidopsis thaliana
Genetic Insert: auxin-inducible degron (aid)
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_169358 Copy
Species: Arabidopsis thaliana
Genetic Insert: Arabidopsis U6 snRNA promoter
Vector Backbone Description: Backbone Marker:Marillonnet, IPB; Backbone Size:2069; Vector Backbone:pAGM9121; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:33898975
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.04.03.023036v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_153220 Copy
Species: Arabidopsis thaliana
Genetic Insert: Arabidopsis Rps5a promoter, Cas9 (Zcasi), nos terminator
Vector Backbone Description: Backbone Marker:Marillonnet, Icon Genetics; Backbone Size:4352; Vector Backbone:pICH47742; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33898975
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.04.03.023036v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_153224 Copy
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