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Species: Other
Genetic Insert: araC
Vector Backbone Description: Backbone Marker:BioBrick vector, IGEM registry; Backbone Size:3252; Vector Backbone:pSB3T5; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Tetracycline
Defining Citation: PMID:32251274
Proper citation: RRID:Addgene_138237 Copy
Species: Other
Genetic Insert: araC
Vector Backbone Description: Backbone Marker:BioBrick vector, IGEM registry; Backbone Size:3252; Vector Backbone:pSB3T5; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Tetracycline
Defining Citation: PMID:32251274
Proper citation: RRID:Addgene_138239 Copy
Species: Other
Genetic Insert: araC
Vector Backbone Description: Backbone Marker:BioBrick vector, IGEM registry; Backbone Size:3252; Vector Backbone:pSB3T5; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Tetracycline
Defining Citation: PMID:32251274
Proper citation: RRID:Addgene_138241 Copy
Species: Other
Genetic Insert: araC
Vector Backbone Description: Backbone Marker:BioBrick vector, IGEM registry; Backbone Size:3252; Vector Backbone:pSB3T5; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Tetracycline
Defining Citation: PMID:32251274
Proper citation: RRID:Addgene_138240 Copy
Species: Other
Genetic Insert: araC
Vector Backbone Description: Backbone Marker:BioBrick vector, IGEM registry; Backbone Size:3252; Vector Backbone:pSB3T5; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Tetracycline
Defining Citation: PMID:32251274
Proper citation: RRID:Addgene_138244 Copy
Species: Aequorea
Genetic Insert: Gfp-mut3.1-GusA
Vector Backbone Description: Backbone Size:10568; Vector Backbone:pJP2; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:16207908
Proper citation: RRID:Addgene_14473 Copy
Species: Synthetic
Genetic Insert: pheS
Vector Backbone Description: Vector Backbone:pDAI-SceI (pBBR1-based); Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:25795676
Proper citation: RRID:Addgene_111611 Copy
Species: Synthetic
Genetic Insert: mRuby2
Vector Backbone Description: Backbone Marker:Dieter Haas; obtained from Culture Collection of Switzerland; Vector Backbone:pME6031; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:29449848
Proper citation: RRID:Addgene_111250 Copy
Species: Synthetic
Genetic Insert: TorT signal peptide
Vector Backbone Description: Backbone Marker:Dieter Haas; obtained from Culture Collection of Switzerland; Vector Backbone:pME6031; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:29449848
Proper citation: RRID:Addgene_111255 Copy
Species: Synthetic
Genetic Insert: E2-Crimson
Vector Backbone Description: Backbone Marker:Dieter Haas; obtained from Culture Collection of Switzerland; Vector Backbone:pME6031; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:29449848
Proper citation: RRID:Addgene_111258 Copy
Species: unknown
Genetic Insert: ccdB-cassette
Vector Backbone Description: Vector Backbone:pTAU_C_DM; Vector Types:Yeast Expression; Bacterial Resistance:Tetracycline
Proper citation: RRID:Addgene_111231 Copy
Species: unknown
Genetic Insert: ccdB-cassette
Vector Backbone Description: Vector Backbone:pBTM116-D9; Vector Types:Yeast Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:22218854
Proper citation: RRID:Addgene_111232 Copy
Species: Mesoplasma florum
Genetic Insert: oriC4 of M. florum strain L1 (rpmH/dnaA and dnaA/dnaN intergenic regions, with dnaA)
Vector Backbone Description: Vector Backbone:colE1; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Tetracycline
Defining Citation: PMID:28115382
Proper citation: RRID:Addgene_101314 Copy
Species: Mesoplasma florum
Genetic Insert: oriC4 of M. florum strain L1 (rpmH/dnaA and dnaA/dnaN intergenic regions, with dnaA)
Vector Backbone Description: Vector Backbone:colE1; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Tetracycline
Defining Citation: PMID:28115382
Proper citation: RRID:Addgene_101315 Copy
Species: Mesoplasma florum
Genetic Insert: oriC4 of M. florum strain L1 (rpmH/dnaA and dnaA/dnaN intergenic regions, with dnaA)
Vector Backbone Description: Vector Backbone:colE1; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Tetracycline
Defining Citation: PMID:28115382
Proper citation: RRID:Addgene_101312 Copy
Species: Mesoplasma florum
Genetic Insert: oriC1 of M. florum strain L1 (rpmH/dnaA intergenic region)
Vector Backbone Description: Vector Backbone:colE1; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Tetracycline
Defining Citation: PMID:28115382
Proper citation: RRID:Addgene_101309 Copy
Vector Backbone Description: Vector Backbone:none; Vector Types:; Bacterial Resistance:Tetracycline
Defining Citation: PMID:29164072
Comments: Genotype = ΔompA ΔompC ΔompF ΔlamB
Precursor strain = BL21Gold(DE3) [genotype F- ompT hsdS(rB– mB– ) dcm+ Tetr gal λ(DE3) endA Hte]
Supplemental document contains a list of genotypes and PCR primers used for verification of each knocked-out gene.
Due to the strain's growth defect and potential for contamination, Addgene recommends streaking for single colonies from the stab on low salt LB containing tetracycline and then screening colonies by PCR using the Leo Lab's recommended primers to confirm the strain.
Proper citation: RRID:Addgene_102270 Copy
Species: Staphylococcus aureus
Genetic Insert: argCA
Vector Backbone Description: Vector Backbone:pXyl-AgrCA1; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:31792444
Proper citation: RRID:Addgene_128409 Copy
Species: Synthetic
Genetic Insert: mRFP1
Vector Backbone Description: Vector Backbone:pBBR1MCS-2; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Tetracycline
Defining Citation: PMID:34333160
Comments: Plasmid parts were assembled using Golden gate method
Proper citation: RRID:Addgene_134884 Copy
Species: S. pyogenes
Genetic Insert: S. pyogenes dCas9
Vector Backbone Description: Backbone Marker:Andrew Hogan; Backbone Size:7649; Vector Backbone:pAH-CTX1-rha; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Tetracycline
Defining Citation: PMID:31491085
Proper citation: RRID:Addgene_129392 Copy
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