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Vector Backbone Description: Vector Backbone:piggyBac-Puro; Vector Types:Mammalian Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39020177
Proper citation: RRID:Addgene_179157 Copy
Species: Synthetic
Genetic Insert: er-(n2)oxStayGold(c4)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35468954
Proper citation: RRID:Addgene_185822 Copy
Genetic Insert: BFP
Vector Backbone Description: Backbone Size:3125; Vector Backbone:pBS; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:27729526
Proper citation: RRID:Addgene_86457 Copy
Species: Danio rerio
Genetic Insert: npas4l
Vector Backbone Description: Backbone Size:4063; Vector Backbone:pCS2; Vector Types:mRNA synthesis; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36044573
Comments: Addgene NGS result had deletion of amino acids 349-352 (TSSS). Depositor confirmed that this deletion does not affect the plasmid function.
Proper citation: RRID:Addgene_164654 Copy
Genetic Insert: mCherry
Vector Backbone Description: Vector Backbone:pcS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33912378
Proper citation: RRID:Addgene_178425 Copy
Species: Synthetic
Genetic Insert: er-(n2)oxStayGold(c4)v2.0
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35468954
Proper citation: RRID:Addgene_186296 Copy
Species: CTG-clade compatible
Genetic Insert: Cas9
Vector Backbone Description: Vector Backbone:pDIV116; Vector Types:Bacterial Expression, Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34746438
Proper citation: RRID:Addgene_177700 Copy
Vector Backbone Description: Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35384245
Proper citation: RRID:Addgene_185481 Copy
Species: E. coli
Genetic Insert: 1-phosphofructokinase
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:4600; Vector Backbone:pET3a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10833389
Proper citation: RRID:Addgene_186256 Copy
Species: Synthetic
Genetic Insert: Synthetic construct isolate AAV9.452sub.LUNG1 VP1 gene
Vector Backbone Description: Backbone Size:6311; Vector Backbone:pUC57-mini; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35990749
Comments: This plasmid is based on the pUCmini-iCAP-PHP.eB plasmid. Please note that this is a non-standard rep-cap construct. It uses a tTA-TRE amplification loop to increase Capsid expression and AAV production. We find that this system increases AAV titers 1.5-5 fold (Ben Deverman, Bryan Simpson and Paul Patterson, unpublished data). This is a tet-off system, so no dox or tet is needed to turn it on. It can be used like any other rep-cap plasmid. This system should only present a problem if the rAAV genome to be packaged has a tet responsive element that directs expression of a protein that affected the health of the production cells. The introduction of an XbaI restriction site for ease of cloning introduces a K449R mutation, which does not have an overt effect on vector production or transduction.
Proper citation: RRID:Addgene_184592 Copy
Species: Mus musculus
Genetic Insert: Caspase-11 (AA100-373)
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34734155
Proper citation: RRID:Addgene_183382 Copy
Species: Synthetic
Genetic Insert: mCherry-CAAX
Vector Backbone Description: Backbone Marker:InvivoGen; Backbone Size:6491; Vector Backbone:pVITRO1-hygro-mcs; Vector Types:Mammalian Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:34838160
Proper citation: RRID:Addgene_186347 Copy
Species: Homo sapiens
Genetic Insert: MOSPD2 motile sperm domain containing 2
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:7181; Vector Backbone:pQCXIP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29858488
Proper citation: RRID:Addgene_186467 Copy
Species: Homo sapiens
Genetic Insert: NPR1
Vector Backbone Description: Backbone Marker:Invitrogen; purchased via GenScript; Backbone Size:4682; Vector Backbone:pFastBac1; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35821229
Comments: N-terminal protein sequence is MKTIIALSYIFCLVFA (hemagglutinin [HA] signal peptide) + HHHHHHHHHH + GAP (from AscI site) + ENLYFQ*G (TEV protease cleavage site; cleavage at * leaves an extra G residue at the N-terminus of pGC-A).
Backbone vector is pFastBac1.
Construct design by Shangji Zhang.
Generate the bacmid in Escherichia coli strain DH10Bac cells according to the instructions from Invitrogen.
Proper citation: RRID:Addgene_186626 Copy
Species: Mus musculus
Genetic Insert: Caspase-11
Vector Backbone Description: Backbone Size:6400; Vector Backbone:pMSCV-IRES-EGFP; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34734155
Proper citation: RRID:Addgene_183358 Copy
Species: Homo sapiens
Genetic Insert: PKCδ(1-229)-eDHFR(69K6)-PKCδ(280-675)-EGFP
Vector Backbone Description: Backbone Marker:Dr. Jun-ichi Miyazaki (Osaka University, Japan); Backbone Size:4891; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35835118
Comments: The chemical mDcTMP, to control protein translocation, can be obtained from Funakoshi Co., Ltd. https://www.funakoshi.co.jp/exports_contents/95015. Please visit https://www.biorxiv.org/content/10.1101/2021.03.16.435568v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_178857 Copy
Species: Homo sapiens
Genetic Insert: PKCδ(1-229)-eDHFR(69K6)-PKCδ(280-675)-miRFP670
Vector Backbone Description: Backbone Marker:Dr. Jun-ichi Miyazaki (Osaka University, Japan); Backbone Size:4891; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35835118
Comments: The chemical mDcTMP, to control protein translocation, can be obtained from Funakoshi Co., Ltd. https://www.funakoshi.co.jp/exports_contents/95015. Please visit https://www.biorxiv.org/content/10.1101/2021.03.16.435568v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_178858 Copy
Species: Homo sapiens
Genetic Insert: PKCδ
Vector Backbone Description: Backbone Marker:Dr. Jun-ichi Miyazaki (Osaka University, Japan); Backbone Size:4891; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35835118
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.03.16.435568v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_178856 Copy
Species: Synthetic
Genetic Insert: mNeonGreen-eDHFR(69K6)
Vector Backbone Description: Backbone Marker:Dr. Hiroyuki Miyoshi (RIKEN, Japan); Backbone Size:10345; Vector Backbone:pCSII-EF-MCS; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35835118
Comments: The chemical mDcTMP, to control protein translocation, can be obtained from Funakoshi Co., Ltd. https://www.funakoshi.co.jp/exports_contents/95015. Please visit https://www.biorxiv.org/content/10.1101/2021.03.16.435568v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_178853 Copy
Species: Homo sapiens
Genetic Insert: mNeonGreen-eDHFR(69K6)-Gαq
Vector Backbone Description: Backbone Marker:Dr. Jun-ichi Miyazaki (Osaka University, Japan); Backbone Size:4912; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35835118
Comments: The chemical mDcTMP, to control protein translocation, can be obtained from Funakoshi Co., Ltd. https://www.funakoshi.co.jp/exports_contents/95015. Please visit https://www.biorxiv.org/content/10.1101/2021.03.16.435568v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_172106 Copy
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