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Vector Backbone Description: Backbone Marker:Addgene Plasmid #65656; Backbone Size:9315; Vector Backbone:LRG; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29526696
Comments: BsmBI digestion for sgRNA cloning
Proper citation: RRID:Addgene_108098 Copy
Species: Homo sapiens
Genetic Insert: mCherry:SACM1L:FKBP1A(3-108)
Vector Backbone Description: Vector Backbone:pmCherry-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29461204
Comments: Note: The Sac1 insert contains a Y434F mutation compared to the closest reference sequence (NP_054735.3). The depositor has noted that this is a common point mutation that does not affect plasmid function.
Proper citation: RRID:Addgene_108129 Copy
Species: Homo sapiens
Genetic Insert: mCherry:FKBP1A(3-108):[GGSA]4GG:SACM1LC389S
Vector Backbone Description: Vector Backbone:pmCherry-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29461204
Comments: Note: The Sac1 insert contains a Y434F mutation compared to the closest reference sequence (NP_054735.3). The depositor has noted that this is a common point mutation that does not affect plasmid function.
Proper citation: RRID:Addgene_108128 Copy
Species: Homo sapiens
Genetic Insert: mCherry:VAPB
Vector Backbone Description: Vector Backbone:pmCherry-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29461204
Proper citation: RRID:Addgene_108126 Copy
Species: Homo sapiens
Genetic Insert: PKD
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12505989
Comments: The HA tag on this plasmid contains a point mutation that renders it unrecognizable by the 3F10 HA antibody. It will work fine with the 12CA5 HA antibody.
The PKD insert in this plasmid contains a L520P mutation when compared to GenBank reference sequence NP_002733.1. Although the effect of this mutation is unknown, this is the exact plasmid used in the associated publication and it should function as described.
Proper citation: RRID:Addgene_10808 Copy
Species: Bos taurus
Genetic Insert: PKC alpha
Vector Backbone Description: Backbone Size:4650; Vector Backbone:pCMV6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12613914
Comments: Constitutively active. Myr sequence from p60src.
Proper citation: RRID:Addgene_10807 Copy
Vector Backbone Description: Backbone Size:7210; Vector Backbone:LentiV; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29526696
Proper citation: RRID:Addgene_108101 Copy
Species: Homo sapiens
Genetic Insert: PKD
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12637538
Comments: The HA tag on this plasmid contains a point mutation that renders it unrecognizable by the 3F10 HA antibody. It will work fine with the 12CA5 HA antibody.
The PKD insert in this plasmid contains a Myc tag inserted between amino acids 33&34, Q66R, F139S and G877W mutations when compared to GenBank reference sequence NP_002733.1. Although the effect of these mutations is unknown, this is the exact plasmid used in the associated publication and it should function as described.
Proper citation: RRID:Addgene_10809 Copy
Species: Synthetic
Genetic Insert: Cas9
Vector Backbone Description: Backbone Size:7000; Vector Backbone:LentiV_puro; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29526696
Proper citation: RRID:Addgene_108100 Copy
Species: Rattus norvegicus
Genetic Insert: PKC zeta
Vector Backbone Description: Backbone Size:4650; Vector Backbone:pCMV5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9768361
Proper citation: RRID:Addgene_10804 Copy
Species: Bos taurus
Genetic Insert: PKC alpha
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12613914
Proper citation: RRID:Addgene_10806 Copy
Species: Other
Genetic Insert: X.leavis map2k1.L(32-44):EGFP:L. penomophila SidM(546-647)
Vector Backbone Description: Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29461204
Proper citation: RRID:Addgene_108121 Copy
Species: B. microti
Genetic Insert: BMR1_01g02031
Vector Backbone Description: Backbone Marker:Yves Durocher; Backbone Size:6670; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30367868
Proper citation: RRID:Addgene_108116 Copy
Species: Homo sapiens
Genetic Insert: LKB1
Vector Backbone Description: Backbone Size:7210; Vector Backbone:LentiV_Neo; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29526696
Proper citation: RRID:Addgene_108111 Copy
Species: Homo sapiens
Genetic Insert: PKD1 RNAi
Vector Backbone Description: Backbone Size:3176; Vector Backbone:pSUPER; Vector Types:Mammalian Expression, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12505989
Comments: Constructed by ligation of the following oligonucleotide pairs to pSUPER: 5'-GATCCCCATG CTGTGGGGGC TGGTACTTCA AGAGAGTACC AGCCCCCACA GCATTTTTTG GAAA-3' and 5'-AGCTTTTCCA AAAAATGCTG TGGGGGCTGG TACTCTCTTG AAGTACCAGC CCCCACAGCA TGGG-3'.
Proper citation: RRID:Addgene_10815 Copy
Species: Homo sapiens
Genetic Insert: PKD
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15024053
Comments: The HA tag on this plasmid contains a point mutation that renders it unrecognizable by the 3F10 HA antibody. It will work fine with the 12CA5 HA antibody.
The PKD insert in this plasmid contains a L520P mutation when compared to GenBank reference sequence NP_002733.1. Although the effect of this mutation is unknown, this is the exact plasmid used in the associated publication and it should function as described.
Proper citation: RRID:Addgene_10814 Copy
Species: Homo sapiens
Genetic Insert: PKD
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12637538
Comments: The HA tag on this plasmid contains a point mutation that renders it unrecognizable by the 3F10 HA antibody. It will work fine with the 12CA5 HA antibody.
The PKD insert in this plasmid contains F94I, V410G and D791G mutations when compared to GenBank reference sequence NP_002733.1. Although the effect of these mutation are unknown, this is the exact plasmid used in the associated publication and it should function as described.
Proper citation: RRID:Addgene_10811 Copy
Vector Backbone Description: Backbone Marker:Jörg Kudla; Backbone Size:14556; Vector Backbone:UBQ10:sXVE:GFPc:Kan; Vector Types:Plant Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29451720
Proper citation: RRID:Addgene_108178 Copy
Vector Backbone Description: Backbone Marker:Jörg Kudla; Backbone Size:14452; Vector Backbone:UBQ10:sXVE:GFPc:Kan; Vector Types:Plant Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29451720
Proper citation: RRID:Addgene_108177 Copy
Species: Synthetic
Genetic Insert: dCas9-dead Tet1CD-HA-P2A-BFP
Vector Backbone Description: Backbone Size:9216; Vector Backbone:Fuw; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29456084
Proper citation: RRID:Addgene_108246 Copy
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