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Genetic Insert: Green fluorescent protein
Vector Backbone Description: Vector Backbone:pET; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25386923
Proper citation: RRID:Addgene_61340 Copy
Species: Synthetic
Genetic Insert: S.pyogenes dCas9 with c-terminal HA tag
Vector Backbone Description: Backbone Marker:Invitrogen; Life Technologies; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25849900
Proper citation: RRID:Addgene_61355 Copy
Vector Backbone Description: Backbone Size:6911; Vector Backbone:pNeuroD-Ires-GFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19737524
Proper citation: RRID:Addgene_61403 Copy
Species: Homo sapiens
Genetic Insert: KCC2
Vector Backbone Description: Backbone Size:6900; Vector Backbone:pCITF; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19376067
Proper citation: RRID:Addgene_61404 Copy
Species: Homo sapiens
Genetic Insert: OTUD1
Vector Backbone Description: Backbone Marker:OPPF-UK; Backbone Size:6299; Vector Backbone:pOPINK; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23827681
Proper citation: RRID:Addgene_61405 Copy
Vector Backbone Description: Backbone Marker:Sigma-Aldrich; Vector Backbone:pZdonor; Vector Types:Mammalian Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25849900
Proper citation: RRID:Addgene_61366 Copy
Species: Homo sapiens
Genetic Insert: OTUD1
Vector Backbone Description: Backbone Marker:OPPF-UK; Backbone Size:6299; Vector Backbone:pOPINK; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23827681
Comments: Hospenthal MK, Mevissen TET, Komander D. Nature Protoc. 2015 Jan 29;10:349-361. doi:10.1038/nprot.2015.018
Proper citation: RRID:Addgene_61406 Copy
Species: Homo sapiens
Genetic Insert: Inhibitor-3
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5800; Vector Backbone:pcDNA5FRT/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25298395
Comments: resistant against siRNA oligomer I3 S2 (CUGCUGTAUUUAUGAGAAA)
Proper citation: RRID:Addgene_61400 Copy
Species: Homo sapiens
Genetic Insert: OTUD2
Vector Backbone Description: Backbone Marker:OPPF-UK; Backbone Size:6299; Vector Backbone:pOPINK; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23827681
Comments: Hospenthal MK, Mevissen TET, Komander D. Nature Protoc. 2015 Jan 29;10:349-361. doi:10.1038/nprot.2015.018
Proper citation: RRID:Addgene_61409 Copy
Species: Homo sapiens
Genetic Insert: S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300)
Vector Backbone Description: Backbone Marker:Invitrogen; Life Technologies; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25849900
Proper citation: RRID:Addgene_61361 Copy
Species: Homo sapiens
Genetic Insert: S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300)
Vector Backbone Description: Backbone Marker:Invitrogen; Life Technologies; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25849900
Proper citation: RRID:Addgene_61364 Copy
Species: Homo sapiens
Genetic Insert: S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300)
Vector Backbone Description: Backbone Marker:Invitrogen; Life Technologies; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25849900
Proper citation: RRID:Addgene_61363 Copy
Species: Homo sapiens
Genetic Insert: S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300)
Vector Backbone Description: Backbone Marker:Invitrogen; Life Technologies; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25849900
Proper citation: RRID:Addgene_61362 Copy
Species: Homo sapiens
Genetic Insert: ALG13 (Putative bifunctional UDP-N-acetylglucosamine transferase and deubiquitinase ALG13)
Vector Backbone Description: Backbone Marker:OPPF-UK; Backbone Size:6299; Vector Backbone:pOPINK; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23827681
Proper citation: RRID:Addgene_61414 Copy
Genetic Insert: QS
Vector Backbone Description: Backbone Marker:Life Technologies ; Backbone Size:2555; Vector Backbone:pDONR221; Vector Types:; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23792917
Comments: Discrepancies between full sequence and Addgene's QC results have no functional consequence.
Proper citation: RRID:Addgene_61378 Copy
Species: Homo sapiens
Genetic Insert: OTUD6A
Vector Backbone Description: Backbone Marker:OPPF-UK; Backbone Size:6299; Vector Backbone:pOPINK; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23827681
Proper citation: RRID:Addgene_61416 Copy
Species: Homo sapiens
Genetic Insert: Full length human RIP3 with V458Pmutation
Vector Backbone Description: Vector Backbone:pEYFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22817896
Proper citation: RRID:Addgene_61377 Copy
Species: Homo sapiens
Genetic Insert: OTUD3
Vector Backbone Description: Backbone Marker:OPPF-UK; Backbone Size:6299; Vector Backbone:pOPINK; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23827681
Comments: Hospenthal MK, Mevissen TET, Komander D. Nature Protoc. 2015 Jan 29;10:349-361. doi:10.1038/nprot.2015.018
Proper citation: RRID:Addgene_61412 Copy
Species: Neurospora
Genetic Insert: QF
Vector Backbone Description: Backbone Marker:Life Technologies; Backbone Size:2564; Vector Backbone:pDONR221; Vector Types:; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23792917
Proper citation: RRID:Addgene_61375 Copy
Species: Synthetic
Genetic Insert: dCAS9(D10A, N863A)-VP64_2A_Blast
Vector Backbone Description: Vector Backbone:plenti; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25494202
Comments: IMPORTANT NOTE: If you intend to order the SAM library, this plasmid will come included with that order. Please do NOT order this plasmid if you are already planning to order the SAM library.
For additional information, protocols and an activator sgRNA design tool, visit our website:
http://sam.genome-engineering.org/
Proper citation: RRID:Addgene_61425 Copy
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