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Genetic Insert: APEX2
Vector Backbone Description: Vector Backbone:pRibo BsaHind; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29708735
Comments: This plasmid is for theophylline-inducible expression of APEX2 in the cytoplasm of mycobacteria. It is episomal and multi-copy. The APEX gene was subcloned from pcDNA3-mito-APEX and subsequently mutated by site-directed mutagenesis to generate the optimized variant APEX2.
See also references: Rhee HW, Zou P, Udeshi ND, Martell JD, Mootha VK, Carr SA, Ting AY. Proteomic mapping of mitochondria in living cells via spatially restricted enzymatic tagging. Science. 2013;339(6125):1328-31. PMCID: 3916822.
Lam SS, Martell JD, Kamer KJ, Deerinck TJ, Ellisman MH, Mootha VK, Ting AY. Directed evolution of APEX2 for electron microscopy and proximity labeling. Nat Methods. 2015;12(1):51-4. PMCID: 4296904
Proper citation: RRID:Addgene_111698 Copy
Genetic Insert: APEX2
Vector Backbone Description: Vector Backbone:pRibo BsaHind; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29708735
Comments: This plasmid is for theophylline-inducible expression of APEX2 in the periplasm of mycobacteria. It is episomal and multi-copy. The APEX gene was subcloned from pcDNA3-mito-APEX and subsequently mutated by site-directed mutagenesis to generate the optimized variant APEX2.
See also references: Rhee HW, Zou P, Udeshi ND, Martell JD, Mootha VK, Carr SA, Ting AY. Proteomic mapping of mitochondria in living cells via spatially restricted enzymatic tagging. Science. 2013;339(6125):1328-31. PMCID: 3916822.
Lam SS, Martell JD, Kamer KJ, Deerinck TJ, Ellisman MH, Mootha VK, Ting AY. Directed evolution of APEX2 for electron microscopy and proximity labeling. Nat Methods. 2015;12(1):51-4. PMCID: 4296904
Proper citation: RRID:Addgene_111699 Copy
Species: Homo sapiens
Genetic Insert: HTT
Vector Backbone Description: Vector Backbone:pBacMam2-DiEx-LIC; Vector Types:Mammalian Expression, Baculovirus Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30842263
Comments: Please visit https://www.biorxiv.org/content/early/2018/12/10/492215 for BioRxiv preprint.
Proper citation: RRID:Addgene_111732 Copy
Species: Homo sapiens
Genetic Insert: HTT
Vector Backbone Description: Vector Backbone:pBacMam2-DiEx-LIC; Vector Types:Mammalian Expression, Baculovirus Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30842263
Comments: Please visit https://www.biorxiv.org/content/early/2018/12/10/492215 for BioRxiv preprint.
Proper citation: RRID:Addgene_111737 Copy
Species: Homo sapiens
Genetic Insert: HTT
Vector Backbone Description: Vector Backbone:pBacMam2-DiEx-LIC; Vector Types:Mammalian Expression, Baculovirus Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30842263
Comments: Please visit https://www.biorxiv.org/content/early/2018/12/10/492215 for BioRxiv preprint.
Please screen multiple clones and validate plasmid by sequencing or restriction analysis before proceeding with downstream experiments
Proper citation: RRID:Addgene_111738 Copy
Species: Homo sapiens
Genetic Insert: HTT
Vector Backbone Description: Vector Backbone:pBacMam2-DiEx-LIC; Vector Types:Mammalian Expression, Baculovirus Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30842263
Comments: Please visit https://www.biorxiv.org/content/early/2018/12/10/492215 for BioRxiv preprint.
Proper citation: RRID:Addgene_111736 Copy
Species: Homo sapiens
Genetic Insert: BLM
Vector Backbone Description: Backbone Marker:ThermoFisher; Vector Backbone:pYES2; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9388193
Proper citation: RRID:Addgene_111722 Copy
Species: Homo sapiens
Genetic Insert: HTT
Vector Backbone Description: Vector Backbone:pBacMam2-DiEx-LIC; Vector Types:Mammalian Expression, Baculovirus Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30842263
Comments: Please visit https://www.biorxiv.org/content/early/2018/12/10/492215 for BioRxiv preprint.
Please screen multiple clones and validate plasmid by sequencing or restriction analysis before proceeding with downstream experiments
Proper citation: RRID:Addgene_111725 Copy
Species: Homo sapiens
Genetic Insert: HTT
Vector Backbone Description: Vector Backbone:pBacMam2-DiEx-LIC; Vector Types:Mammalian Expression, Baculovirus Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30842263
Comments: Please visit https://www.biorxiv.org/content/early/2018/12/10/492215 for BioRxiv preprint.
Proper citation: RRID:Addgene_111729 Copy
Species: Homo sapiens
Genetic Insert: hSesn2
Vector Backbone Description: Vector Backbone:pLU-CMV; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26612684
Proper citation: RRID:Addgene_111792 Copy
Species: Homo sapiens
Genetic Insert: hSesn2
Vector Backbone Description: Vector Backbone:pLU-CMV; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26612684
Proper citation: RRID:Addgene_111790 Copy
Species: Mus musculus
Genetic Insert: mSesn2
Vector Backbone Description: Vector Backbone:pcDNA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25040165
Proper citation: RRID:Addgene_111796 Copy
Species: Mus musculus
Genetic Insert: mSesn2
Vector Backbone Description: Vector Backbone:pcDNA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25040165
Proper citation: RRID:Addgene_111797 Copy
Species: Mus musculus
Genetic Insert: mSesn2
Vector Backbone Description: Vector Backbone:pcDNA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25040165
Proper citation: RRID:Addgene_111798 Copy
Species: Synthetic
Genetic Insert: D44.1
Vector Backbone Description: Backbone Size:6168; Vector Backbone:pCTcon2; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31442220
Comments: The amino acid sequence is taken from the deposited structure 1MLC in the protein data bank.
Proper citation: RRID:Addgene_111718 Copy
Genetic Insert: sgRNA targeting position 6 in pColE1_70a_deGFP_KanR
Vector Backbone Description: Vector Backbone:YTK095; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32995532
Comments: Marshall et al. 2018 (PMID: 29304331)
Proper citation: RRID:Addgene_119149 Copy
Genetic Insert: sgRNA targeting position 3 in pColE1_70a_deGFP_KanR
Vector Backbone Description: Vector Backbone:YTK095; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32995532
Comments: Marshall et al. 2018 (PMID: 29304331)
Proper citation: RRID:Addgene_119146 Copy
Genetic Insert: non-targeting sgRNA
Vector Backbone Description: Vector Backbone:YTK095; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32995532
Comments: Marshall et al. 2018 (PMID: 29304331)
Proper citation: RRID:Addgene_119144 Copy
Genetic Insert: VSVG
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4733; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:9288971
Comments: The EGFP (Clontech Laboratories, Inc., Palo, Alto, CA) contains a valine immediately after the start codon that is not found in the wild type sequence. However, to avoid confusion with previously published work on GFP mutants, the wild type residue numbers are used. The Kozak sequence at the beginning of the GFP encoding region of the pEGFP-N1 plasmid from Clontech has been disrupted. Reference for the A206K mutation is Zacharias, D. A., Violin, J. D., Newton, A. C., and Tsien, R. Y. (2002) Science 296, 913-6. Reference for PAGFP is Patterson, G. H., and Lippincott-Schwartz, J. (2002) Science 297, 1873-7.
Proper citation: RRID:Addgene_11915 Copy
Genetic Insert: sgRNA targeting position 8 in pColE1_70a_deGFP_KanR
Vector Backbone Description: Vector Backbone:YTK095; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32995532
Comments: Marshall et al. 2018 (PMID: 29304331)
Proper citation: RRID:Addgene_119151 Copy
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