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Species: bacteriophage P1
Genetic Insert: GFP-cre
Vector Backbone Description: Backbone Size:5000; Vector Backbone:na; Vector Types:Mammalian Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10334853
Comments: pBS596 differs from pBS537 in having the MT-I A(n) instead of the GCSF A(n). MT-I A(n) is the mouse metallothionein MT-I region including the polyadenylation site and several introns. pBS537 carries a GFPcre fusion gene under the control of a synthetic promoter that can be regulated by a synthetic tet repressor/activator protein. In the presence of doxycycline expression is dramatically turned on to high levels. The GFP moiety carries the S65T mutation for enhanced fluorescence, but is not codon-optimized for mammalian expression.
Proper citation: RRID:Addgene_11961 Copy
Species: Synthetic
Genetic Insert: UP Flank Linker
Vector Backbone Description: Backbone Size:2247; Vector Backbone:pICH41331; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30819783
Comments: Please visit https://www.biorxiv.org/content/10.1101/426700v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_119613 Copy
Species: Synechocystis sp. PCC6803
Genetic Insert: SspDnaEC
Vector Backbone Description: Backbone Marker:invitrogen; Backbone Size:4766; Vector Backbone:pBAD; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15908942
Proper citation: RRID:Addgene_11964 Copy
Genetic Insert: TrrnB
Vector Backbone Description: Backbone Size:2247; Vector Backbone:PICH41276; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30819783
Comments: E. coli double terminator rrnB T1 and T7Te. Please visit https://www.biorxiv.org/content/10.1101/426700v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_119611 Copy
Species: Synechocystis sp. PCC6803
Genetic Insert: SspDnaEC
Vector Backbone Description: Backbone Marker:invitrogen; Backbone Size:4754; Vector Backbone:pBAD; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15908942
Proper citation: RRID:Addgene_11963 Copy
Species: Synthetic
Genetic Insert: AbR Down Linker
Vector Backbone Description: Backbone Size:2247; Vector Backbone:pICH1276; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30819783
Comments: Please visit https://www.biorxiv.org/content/10.1101/426700v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_119616 Copy
Species: Synthetic
Genetic Insert: AbR Up Linker
Vector Backbone Description: Backbone Size:2247; Vector Backbone:pICH41331 ; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30819783
Comments: Please visit https://www.biorxiv.org/content/10.1101/426700v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_119617 Copy
Species: Homo sapiens
Genetic Insert: NEMO
Vector Backbone Description: Backbone Size:5370; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16547522
Proper citation: RRID:Addgene_11966 Copy
Species: Homo sapiens
Genetic Insert: NEMOL329P
Vector Backbone Description: Backbone Size:4900; Vector Backbone:pGEX4T1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16547522
Proper citation: RRID:Addgene_11967 Copy
Species: Synthetic
Genetic Insert: Pcat
Vector Backbone Description: Backbone Size:2247; Vector Backbone:PICH41295; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30819783
Comments: Promoter for the chloramphenicol acetyl transferase (CAT) gene in Salmonella enterica. Please visit https://www.biorxiv.org/content/10.1101/426700v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_119600 Copy
Genetic Insert: Tcpc_operon
Vector Backbone Description: Backbone Size:2247; Vector Backbone:PICH41276; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30819783
Comments: Please visit https://www.biorxiv.org/content/10.1101/426700v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_119609 Copy
Species: Synthetic
Genetic Insert: J23108_TSS
Vector Backbone Description: Backbone Size:2247; Vector Backbone:PICH41295; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30819783
Comments: Please visit https://www.biorxiv.org/content/10.1101/426700v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_119605 Copy
Species: Synthetic
Genetic Insert: Ptic
Vector Backbone Description: Backbone Size:3313; Vector Backbone:pSB4K5; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30819783
Comments: Synthetic variant of the tic promoter from E. coli. Please visit https://www.biorxiv.org/content/10.1101/426700v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_119603 Copy
Species: Synthetic
Genetic Insert: J23103_TSS
Vector Backbone Description: Backbone Size:2247; Vector Backbone:PICH41295; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30819783
Comments: Addgene QC NGS identified sequence discrepancies in the plasmid insert. The plasmid is expected to function as described in the associated publication.
Please visit https://www.biorxiv.org/content/10.1101/426700v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_119604 Copy
Vector Backbone Description: Backbone Marker:Yongsub Kim; Backbone Size:3161; Vector Backbone:pFGRNA; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29335559
Proper citation: RRID:Addgene_119674 Copy
Genetic Insert: g17_U6_g4_U6_NOS_NLS_GB1_NLS_linker_VP64_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_2xNLS_linker_10xGCN4_OCS
Vector Backbone Description: Vector Backbone:pEG302; Vector Types:Plant Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30760722
Proper citation: RRID:Addgene_119672 Copy
Species: Streptococcus pyogenes
Genetic Insert: Cas9
Vector Backbone Description: Vector Backbone:p15A origin; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29982721
Proper citation: RRID:Addgene_119701 Copy
Species: Synthetic
Genetic Insert: mKelly1
Vector Backbone Description: Backbone Size:5153; Vector Backbone:pET-53-DEST; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30425172
Proper citation: RRID:Addgene_119782 Copy
Genetic Insert: PtrpC:hph:Ttub
Vector Backbone Description: Backbone Marker:Diego Orzaez; Backbone Size:6965; Vector Backbone:PDGB3alpha1 (Addgene #68218); Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29680684
Proper citation: RRID:Addgene_119708 Copy
Genetic Insert: AtU6-SmR-sgRNA
Vector Backbone Description: Backbone Size:3152; Vector Backbone:pUC57; Vector Types:Plant Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30272679
Proper citation: RRID:Addgene_119775 Copy
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