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Genetic Insert: MDmut-mTurquoise
Vector Backbone Description: Vector Backbone:FUW; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37580379
Proper citation: RRID:Addgene_193095 Copy
Species: Synthetic
Genetic Insert: Optimized neurogenin-2
Vector Backbone Description: Vector Backbone:K4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_204734 Copy
Species: Synthetic
Genetic Insert: Zim3-dCas9
Vector Backbone Description: Vector Backbone:M54; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_204732 Copy
Species: Synthetic
Genetic Insert: Synthetic construct isolate AAV.CAP-Mac VP1 gene
Vector Backbone Description: Vector Backbone:pUC57-mini; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37430038
Comments: Please note that this is a non-standard rep-cap construct. It uses a tTA-TRE amplification loop to increase Capsid expression and AAV production. We find that this system increases AAV titers 1.5-5 fold (Ben Deverman, Bryan Simpson and Paul Patterson, unpublished data). This is a tet-off system, so no dox or tet is needed to turn it on. It can be used like any other rep-cap plasmid. This system should only present a problem if the rAAV genome to be packaged has a tet responsive element that directs expression of a protein that affected the health of the production cells. The introduction of an XbaI restriction site for ease of cloning introduces a K449R mutation, which does not have an overt effect on vector production or transduction.
This work was supported by funding from:
NIH BRAIN Armamentarium UF1MH128336
Michael J Fox Foundation for Parkinson's Research ASAP-020495
Proper citation: RRID:Addgene_200658 Copy
Species: Homo sapiens
Genetic Insert: ATG3
Vector Backbone Description: Vector Backbone:pCDH1-CMV-MCS-SV40-Hygro; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_203641 Copy
Species: Synthetic
Genetic Insert: Zim3-dCas9
Vector Backbone Description: Vector Backbone:M54; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_204730 Copy
Species: Synthetic
Genetic Insert: Zim3-dCas9
Vector Backbone Description: Vector Backbone:M54; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_204731 Copy
Species: Mercenaria mercenaria
Genetic Insert: MmeFz2 omegaRNA
Vector Backbone Description: Vector Backbone:pU6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37380027
Proper citation: RRID:Addgene_205267 Copy
Species: Vibrio cholera
Genetic Insert: VchTniQ, VchCas5/8, VchCas7, VchCas6, VchTnsABC, CRISPR(crtYf)
Vector Backbone Description: Vector Backbone:pJYS1Ptac; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37348112
Comments: Plasmid can also be transformed in Corynebacterium glutamicum, grown at 30 C
Proper citation: RRID:Addgene_203816 Copy
Species: Vibrio cholera
Genetic Insert: Mini-Tn, Spec cassette
Vector Backbone Description: Vector Backbone:pJYS2_crtYf; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:37348112
Comments: Plasmid can also be transformed in Corynebacterium glutamicum, grown at 37 C
Proper citation: RRID:Addgene_203817 Copy
Vector Backbone Description: Backbone Size:10589; Vector Backbone:plex_307; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Proper citation: RRID:Addgene_201129 Copy
Species: Synthetic
Genetic Insert: ALFA tag
Vector Backbone Description: Backbone Size:3761; Vector Backbone:pFA6a; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36190134
Proper citation: RRID:Addgene_201767 Copy
Species: Synthetic
Genetic Insert: ALFA tag
Vector Backbone Description: Backbone Size:3763; Vector Backbone:pFA6a; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36190134
Proper citation: RRID:Addgene_201766 Copy
Species: Synthetic
Genetic Insert: NbALFA
Vector Backbone Description: Backbone Size:6086; Vector Backbone:pRS305; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36190134
Proper citation: RRID:Addgene_201769 Copy
Species: Synthetic
Genetic Insert: NbALFA
Vector Backbone Description: Backbone Size:6516; Vector Backbone:pRS305; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36190134
Proper citation: RRID:Addgene_201768 Copy
Species: Synthetic
Genetic Insert: iRFP713(C15S/V256C)
Vector Backbone Description: Backbone Size:10120; Vector Backbone:pCSIIbleo; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_198062 Copy
Species: Synthetic
Genetic Insert: iRFP670
Vector Backbone Description: Backbone Size:10120; Vector Backbone:pCSIIbleo; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_198060 Copy
Species: Arabidopsis thaliana
Genetic Insert: Phytochrome B Y276H(1-621 aa)
Vector Backbone Description: Backbone Marker:Cell Biolabs Inc; Backbone Size:4600; Vector Backbone:pAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_197377 Copy
Species: Homo sapiens
Genetic Insert: EGFR mutant
Vector Backbone Description: Backbone Size:7000; Vector Backbone:pPBpuro; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37519219
Proper citation: RRID:Addgene_197379 Copy
Species: Synthetic
Genetic Insert: miRFP718nano(S86C)
Vector Backbone Description: Backbone Size:10120; Vector Backbone:pCSIIbleo; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_197373 Copy
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