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Vector Backbone Description: Backbone Marker:invitrogen; Backbone Size:5446; Vector Backbone:pUC18; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20936606
Proper citation: RRID:Addgene_26839 Copy
Vector Backbone Description: Backbone Size:5010; Vector Backbone:pUC18; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20936606
Proper citation: RRID:Addgene_26836 Copy
Vector Backbone Description: Backbone Marker:invitrogen; Backbone Size:5633; Vector Backbone:pUC18; Vector Types:Yeast Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20936606
Proper citation: RRID:Addgene_26835 Copy
Vector Backbone Description: Backbone Size:6115; Vector Backbone:pUC18; Vector Types:Yeast Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20936606
Proper citation: RRID:Addgene_26830 Copy
Vector Backbone Description: Backbone Size:6226; Vector Backbone:pUC18; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20936606
Proper citation: RRID:Addgene_26831 Copy
Species: Mus musculus
Genetic Insert: MyoD-ER(T)
Vector Backbone Description: Backbone Size:6707; Vector Backbone:pRRL-cPPT-X-PRE-SIN; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18511457
Comments: Cloning of the MyoD-ER(T):
Briefly, the full-length mouse MyoD cDNA was isolated and cloned from a previously described adenoviral vector (Lattanzi et al., 1998). A cDNA for the modified estrogen receptor responsive to tamoxifen and 4-hydroxytamoxifen, ER(T) (Danielian et al., 1993), was cloned using a PCR method with the primer pair including an internal ClaI restriction enzyme site: (sense) 5′-ACGGCATCGATTCTGCTGGAGACATGAGAGCT-3′, and (anti-sense) 5′-GCGCCATCGATGACTGTGGCAGGGAAACCCT-3′, and then inserted into the NarI site (amino acid 173) in the middle of the mouse MyoD cDNA. The final clone was verified by DNA sequencing.
There is a mutation G525R in the mouse estrogen receptor; the mutation confers selective 4-hydroxytamoxifen sensitivity (vs. estradiol) (Danielian et al. 1993 Molec Endocrin 7: 232-240).
Proper citation: RRID:Addgene_26809 Copy
Species: Mus musculus
Genetic Insert: MyoD
Vector Backbone Description: Backbone Marker:Lentiviral plasmid backbone from L. Naldini; Backbone Size:6707; Vector Backbone:pRRL-cPPT-X-PRE-SIN; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18511457
Comments: CMV promotor
Proper citation: RRID:Addgene_26808 Copy
Species: C. reinhardtii
Genetic Insert: ChR2
Vector Backbone Description: Backbone Marker:Scott Sternson; Backbone Size:5454; Vector Backbone:AAV with CAG promoter; Vector Types:Mammalian Expression, AAV, adeno-associated virus; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16116447
Proper citation: RRID:Addgene_26929 Copy
Species: Mus musculus
Genetic Insert: autophagy-related 3 (yeast)
Vector Backbone Description: Backbone Size:5169; Vector Backbone:pBABE-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20723759
Proper citation: RRID:Addgene_26926 Copy
Species: Arabidopsis thaliana
Genetic Insert: CRY2-CreN
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4722; Vector Backbone:pMCherryC1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21037589
Proper citation: RRID:Addgene_26888 Copy
Genetic Insert: BIO-tag
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pFA6aTRP1; Vector Types:PCR tagging yeast; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16823883
Comments: There is a short deletion in Addgene's QC sequence relative to the depositor's sequence, but it does not appear to affect the important features.
Proper citation: RRID:Addgene_26881 Copy
Genetic Insert: 18xHis tag
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pFA6akanMX6; Vector Types:PCR tagging yeast; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16823883
Proper citation: RRID:Addgene_26883 Copy
Genetic Insert: 18xHis tag
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pFA6akanMX6; Vector Types:PCR tagging yeast; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16823883
Proper citation: RRID:Addgene_26882 Copy
Species: Homo sapiens
Genetic Insert: 5'UTR-LIN28A-3'UTR
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pcDNA3.3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20888316
Comments: Note that orientation of the insert in pcDNA does not matter -- they were cloned by TA-cloning, and either orientation is fine.
Proper citation: RRID:Addgene_26819 Copy
Species: Homo sapiens
Genetic Insert: 5'UTR-OCT4-3'UTR
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pcDNA3.3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20888316
Comments: Note that OCT4 has some silent mutations.
Also note that orientation of the insert in pcDNA does not matter -- they were cloned by TA-cloning, and either orientation is fine.
Proper citation: RRID:Addgene_26816 Copy
Species: Caenorhabditis elegans
Genetic Insert: dcap-1
Vector Backbone Description: Backbone Size:0; Vector Backbone:unknown; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18692039
Proper citation: RRID:Addgene_26938 Copy
Vector Backbone Description: Backbone Marker:invitrogen; Backbone Size:5883; Vector Backbone:pUC18; Vector Types:Yeast Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20936606
Proper citation: RRID:Addgene_26812 Copy
Species: Xenopus laevis
Genetic Insert: Tektin 2
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18552847
Comments: Addgene's sequencing results identified a I429A mutation in the Tektin 2 sequence when compared to GenBank reference sequence NP_001089680.1
Proper citation: RRID:Addgene_26932 Copy
Species: Caenorhabditis elegans
Genetic Insert: lag-2 promoter
Vector Backbone Description: Backbone Marker:Andrew Fire (Addgene Plasmid #1497); Backbone Size:4492; Vector Backbone:pPD95.81; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7568229
Comments: Quick confirmation: BamHI should result in two fragments: ~3kb and 4.5kb.
This is a lag-2 promoter::GFP fusion. Strong GFP in live worms. 3kb BamHI fragment of pJK520 is the complete 3kb 5' region of lag-2.
Proper citation: RRID:Addgene_26814 Copy
Vector Backbone Description: Backbone Marker:invitrogen; Backbone Size:5260; Vector Backbone:pUC18; Vector Types:Yeast Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20936606
Proper citation: RRID:Addgene_26813 Copy
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