Plasmids   Select Another Resource Report Type

http://www.addgene.org/61000

Species: Thermoanaerobacter tengcongensis MB4
Genetic Insert: TTE1564 transcript
Vector Backbone Description: Backbone Size:2700; Vector Backbone:pUC19; Vector Types:Unspecified; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26781350

Proper citation: RRID:Addgene_61000 Copy   

•   Source: Addgene

http://www.addgene.org/61088

Species: Homo sapiens
Genetic Insert: SENP2M497A catalytic domain
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:7328; Vector Backbone:PET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23955022

Proper citation: RRID:Addgene_61088 Copy   

•   Source: Addgene

http://www.addgene.org/61017

Species: Synthetic
Genetic Insert: ddGFP B
Vector Backbone Description: Backbone Size:5500; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25622108

Proper citation: RRID:Addgene_61017 Copy   

•   Source: Addgene

http://www.addgene.org/61018

Species: Synthetic
Genetic Insert: ddGFP A
Vector Backbone Description: Backbone Size:5500; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25622108

Proper citation: RRID:Addgene_61018 Copy   

•   Source: Addgene

http://www.addgene.org/61093

Species: Mus musculus
Genetic Insert: Mag genomic sequence
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:6273; Vector Backbone:psiCheck2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23704325

Proper citation: RRID:Addgene_61093 Copy   

•   Source: Addgene

http://www.addgene.org/61092

Species: Cricetulus griseus
Genetic Insert: pkac2
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4731; Vector Backbone:EGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:12574406
Comments: The Y307H found in Addgene's QC sequence has no known functional consequence.

Proper citation: RRID:Addgene_61092 Copy   

•   Source: Addgene

http://www.addgene.org/61090

Species: Mus musculus
Genetic Insert: hnRNP A1
Vector Backbone Description: Backbone Size:6700; Vector Backbone:pHMTC; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23704325

Proper citation: RRID:Addgene_61090 Copy   

•   Source: Addgene

http://www.addgene.org/61096

Species: Mus musculus
Genetic Insert: mouse BIN1 with exon 17, excluding exon 7, 11, 13-16
Vector Backbone Description: Backbone Marker:Life Technologies; Backbone Size:8123; Vector Backbone:pDEST27; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24836577

Proper citation: RRID:Addgene_61096 Copy   

•   Source: Addgene

http://www.addgene.org/61026

Species: Equus*
Genetic Insert: horse cytochrome c
Vector Backbone Description: Backbone Marker:Multiple labs; Vector Backbone:pBTR(C102T); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11437597
Comments: Notes from depositor: CYC3 encodes the enzyme, which we call heme lysase in the paper, that covalently attaches the heme to the apocytochrome c. That is, the plasmid has BOTH the CYC3 gene and the structural gene for horse cytochrome c. The horse cytochrome C sequence has been codon optimized.

Proper citation: RRID:Addgene_61026 Copy   

•   Source: Addgene

http://www.addgene.org/61021

Vector Backbone Description: Backbone Marker:Andrew Fire (Addgene plasmid #1490); Backbone Size:4526; Vector Backbone:pPD95_67; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Comments: This vector can be used to create N- terminal or C-terminal mCherry tagged fusion proteins for expression in C. elegans. The mCherry and a modified MCS was inserted into the vector backbone using AgeI and EcoRI sites. See plasmid map and sequence for more information. Note that mCherry has been codon optimized for C. elegans expression. See pCFJ90 (Plasmid #19327) for details on the mCherry protein sequence.

Proper citation: RRID:Addgene_61021 Copy   

•   Source: Addgene

http://www.addgene.org/61151

Vector Backbone Description: Vector Backbone:NA; Vector Types:; Bacterial Resistance:None
Defining Citation: PMID:23479654
Comments: In addition to the wt copy of lacI, another copy of lacI driven by PIq is integrated at the attB Contains another copy of araC driven by a constitutive promoter in addition to wt araC.

Proper citation: RRID:Addgene_61151 Copy   

•   Source: Addgene

http://www.addgene.org/61156

Species: Escherichia coli
Genetic Insert: gusA
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5369; Vector Backbone:pET28a (+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:10404164
Comments: Matsumura lab strain number 337

Proper citation: RRID:Addgene_61156 Copy   

•   Source: Addgene

http://www.addgene.org/61034

Species: Danio rerio
Genetic Insert: HSPB7
Vector Backbone Description: Vector Backbone:pCRII; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18161059
Comments: linearize with NotI, transcribe with SP6, probe length is 519. Note: Addgene's quality control sequencing has found a few mismatches in the plasmid insert, but they should not affect its ability to be used as a probe.

Proper citation: RRID:Addgene_61034 Copy   

•   Source: Addgene

http://www.addgene.org/61154

Species: Saccharomyces cerevisiae
Genetic Insert: TFIIH complex serine/threonine-protein kinase subunit KIN28
Vector Backbone Description: Backbone Marker:Herren, B., and Pech, M. (1993) J. Recept. Res. 13, 725–738; Vector Backbone:pRS425Gal1; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11805111

Proper citation: RRID:Addgene_61154 Copy   

•   Source: Addgene

http://www.addgene.org/60995

Species: Synthetic
Genetic Insert: citrine
Vector Backbone Description: Vector Backbone:pMTB; Vector Types:Zebrafish; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26010570
Comments: Multibow constructs contain repetitive loxP sites that make these constructs prone to recombination. The constructs display a few issues with bacterial stability and sequencing that need to be considered when amplifying, storing and using them. First, in our hands Multibow constructs have displayed instability in E.coli cultures and had low yield in Maxi/Midi-preps. Mini-prep is recommended to harvest Multibow constructs. For transformation, we have had success with 5-alpha F'Iq cells (NEB). Second, the sequencing of Multibow constructs may run into problems of low quality/inaccurate reads. A problematic trace file does not necessarily mean the construct is wrong or the sample has mixed sequences. Third, before using the constructs harvested from mini-prep for injections, we recommend a further purification step using DNA purification kits such as MinElute PCR purification kit (Qiagen). All Multibow constructs share a backbone of pMTB vector (AMP resistance) containing the tol2 sites for transgenic insertion and the loxP recombination sites. To validate the variable region, we recommend sequencing with this specific primer: 5'-CAGCAGGACCATTTATCATGCTGCTGC-3', which recognizes the end of the variable region. The Sp6 primer may not provide enough reading length to reach the variable region.

Proper citation: RRID:Addgene_60995 Copy   

•   Source: Addgene

http://www.addgene.org/60994

Species: Synthetic
Genetic Insert: EGFP
Vector Backbone Description: Vector Backbone:pMTB; Vector Types:Zebrafish; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26010570
Comments: Multibow constructs contain repetitive loxP sites that make these constructs prone to recombination. The constructs display a few issues with bacterial stability and sequencing that need to be considered when amplifying, storing and using them. First, in our hands Multibow constructs have displayed instability in E.coli cultures and had low yield in Maxi/Midi-preps. Mini-prep is recommended to harvest Multibow constructs. For transformation, we have had success with 5-alpha F'Iq cells (NEB). Second, the sequencing of Multibow constructs may run into problems of low quality/inaccurate reads. A problematic trace file does not necessarily mean the construct is wrong or the sample has mixed sequences. Third, before using the constructs harvested from mini-prep for injections, we recommend a further purification step using DNA purification kits such as MinElute PCR purification kit (Qiagen). All Multibow constructs share a backbone of pMTB vector (AMP resistance) containing the tol2 sites for transgenic insertion and the loxP recombination sites. To validate the variable region, we recommend sequencing with this specific primer: 5'-CAGCAGGACCATTTATCATGCTGCTGC-3', which recognizes the end of the variable region. The Sp6 primer may not provide enough reading length to reach the variable region.

Proper citation: RRID:Addgene_60994 Copy   

•   Source: Addgene

http://www.addgene.org/61168

Genetic Insert: NLS-mCherry-GUS
Vector Backbone Description: Vector Backbone:pKm43GW; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:25329881

Proper citation: RRID:Addgene_61168 Copy   

•   Source: Addgene

http://www.addgene.org/60996

Species: Synthetic
Genetic Insert: mKO
Vector Backbone Description: Vector Backbone:pMTB; Vector Types:Zebrafish; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26010570
Comments: Multibow constructs contain repetitive loxP sites that make these constructs prone to recombination. The constructs display a few issues with bacterial stability and sequencing that need to be considered when amplifying, storing and using them. First, in our hands Multibow constructs have displayed instability in E.coli cultures and had low yield in Maxi/Midi-preps. Mini-prep is recommended to harvest Multibow constructs. For transformation, we have had success with 5-alpha F'Iq cells (NEB). Second, the sequencing of Multibow constructs may run into problems of low quality/inaccurate reads. A problematic trace file does not necessarily mean the construct is wrong or the sample has mixed sequences. Third, before using the constructs harvested from mini-prep for injections, we recommend a further purification step using DNA purification kits such as MinElute PCR purification kit (Qiagen). All Multibow constructs share a backbone of pMTB vector (AMP resistance) containing the tol2 sites for transgenic insertion and the loxP recombination sites. To validate the variable region, we recommend sequencing with this specific primer: 5'-CAGCAGGACCATTTATCATGCTGCTGC-3', which recognizes the end of the variable region. The Sp6 primer may not provide enough reading length to reach the variable region.

Proper citation: RRID:Addgene_60996 Copy   

•   Source: Addgene

http://www.addgene.org/61042

Species: Saccharomyces cerevisiae
Genetic Insert: Prp2
Vector Backbone Description: Backbone Marker:Invitrogen Life Technologies; Backbone Size:2900; Vector Backbone:pRSETA; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24240612

Proper citation: RRID:Addgene_61042 Copy   

•   Source: Addgene

http://www.addgene.org/61162

Genetic Insert: See comments
Vector Backbone Description: Vector Backbone:N/A; Vector Types:Synthetic Biology; Bacterial Resistance:None
Defining Citation: PMID:22833608
Comments: MG1655 + PLlacO-1::T710::yfp intergrated at galK

Proper citation: RRID:Addgene_61162 Copy   

•   Source: Addgene