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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
HA-Vim
 
Resource Report
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1+ mentions
RRID:Addgene_46315 Vimentin Mus musculus Ampicillin PMID:19726676 Backbone Marker:Clontech; Backbone Size:3800; Vector Backbone:pCMV-HA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:15:30 1
pEGFPC1/GgVcl 1-258 A50I mutation
 
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1+ mentions
RRID:Addgene_46271 Vcl 1-258 (aka VD1) A50I mutation Gallus gallus Kanamycin PMID:16608855 A50I mutation introduced into EGFP/V1-258 (Addgene plasmid 46270) by QCPCR. Backbone Marker:Clonetech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 1-258 (VD1 domain) A50I mutation 2026-08-15 01:15:30 1
pEGFPC3/GgVcl 884-1066 (Vt)
 
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RRID:Addgene_46272 Vcl 884-1066 (Vt) Gallus gallus Kanamycin PstI-ApaI fragment of pGEX3TV884-1066 subcloned into Pst-Apa cut pEGFP-C3)EGFP. Backbone Marker:Clonetech; Backbone Size:4700; Vector Backbone:pEGFP-C3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 884-1066 (Vt) tail domain 2026-08-15 01:15:30 0
pEGFPC1/GgVcl 1-258 (aka VD1)
 
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1+ mentions
RRID:Addgene_46270 Vcl 1-258 (aka VD1) Gallus gallus Kanamycin PMID:16608855 Nde-Xho fragment from pET15b/Vh1-258 (Addgene plasmid 46173) was subcloned into EGFP/V1-1066 (Addgene plasmid 46265) vector modified to contain unique Nde, Xho sites. Modifications were made by QuikChange PCR to facilitate cloning of cDNAs previously in the pET15b vector. The NdeI site present in the cytomegalovirus promoter region was removed (mutating adenine 234 to thymine), and a new NdeI site was introduced into the multiple cloning site immediately before the vinculin start codon. Additionally, the 5-XhoI site was removed (mutating cytosine 1345 to adenine), and a 3-XhoI site was introduced 3' to the vinculin cDNA in lieu of a PstI site. Backbone Marker:Clonetech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 1-258 (VD1 domain) 2026-08-15 01:15:30 3
mcherry(C1)_RepoMan
 
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RRID:Addgene_46274 RepoMan Homo sapiens Kanamycin PMID:16492807 Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:mcherry-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:15:30 0
pEGFPC1/GgVcl 1-851 A50I mutant
 
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1+ mentions
RRID:Addgene_46269 Vcl 1-851 A50I mutation Gallus gallus Kanamycin PMID:16608855 A50I mutation introduced into EGFP/V1-851 (Addgene plasmid 46268) by QCPCR mutation. Backbone Marker:Clonetech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 1-851 (Vh) head domain, A50I mutation 2026-08-15 01:15:30 2
pEGFPC1/GgVcl 1-1066 T12 mutant
 
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1+ mentions
RRID:Addgene_46266 Vcl T12 mutant Gallus gallus Kanamycin PMID:15728584 T12 mutation introduced by QuikChange PCR to Addgene plasmid 46265. Mutant inhibits vinculin autoinhibition by ~ 100 fold, vinculin is partially activated and will bind talin, but not actin (unless both talin and actin are present at same time). Backbone Marker:Clonetech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin T12 mutant (D974;K975;R976; R978A) autoinhibition decreased by 100x 2026-08-15 01:15:30 2
pEGFPC1/GgVcl 1-1066 A50I K1306A, R1308A mutant
 
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RRID:Addgene_46267 Vcl Gallus gallus Kanamycin Chicken vinculin, full length(EcoRI fragment of p1005 subcloned into EcoRI-cut pEGFP-C1); A50I mutation (alanine 50 to isoleucine) introduced by QCPCR. This mutant binds poorly (undetectably to talin in pull down assays; sensitivity of assay Kd= 10-20 uM). Backbone Marker:Clonetech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin K1306A, R1308A, A50I mutation; talin binding deficient 2026-08-15 01:15:30 0
SI10-Brevican
 
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RRID:Addgene_46300 Brevican mouse monoclonal antibody Danio rerio Ampicillin PMID:20525357 Please note that Addgene's sequencing results contain a few nucleotide mismatches when compared to the sequence provided by the depositing laboratory. These mismatches do not affect the production of recombinant antibodies, nor the affinity of the antibody for its antigen. Backbone Marker:Yves Durocher, NRC; Backbone Size:5925; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:15:30 0
SI15-CD276
 
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RRID:Addgene_46305 CD276 mouse monoclonal antibody Danio rerio Ampicillin PMID:20525357 Backbone Marker:Yves Durocher, NRC; Backbone Size:5925; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:15:30 0
pGEX-4T-1-p19-T7
 
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1+ mentions
RRID:Addgene_46306 p19 Tomato bushy stunt virus Ampicillin PMID:23475073 Backbone Size:4941; Vector Backbone:pGEX-4T-1; Vector Types:Bacterial Expression, pro-siRNA; Bacterial Resistance:Ampicillin 2026-08-15 01:15:30 1
pET15b/EYFP-GgVcl 1-851
 
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RRID:Addgene_46264 Vcl 1-851 Gallus gallus Ampicillin PMID:15728584 EYFP was PCR amplified to introduce NdeI site at both 5' and 3' ends. The NdeI flanked EYFP was inserted into NdeI site of pET15b/Vh1-851 (Addgene plasmid 46172), cloned between NdeI and XhoI. Plasmid contains a 12-residue spacer between the EYFP and vinculin domain. Backbone Marker:EMD; Backbone Size:5700; Vector Backbone:pET-15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin aa 1-851 (aka Vh) head domain 2026-08-15 01:15:30 0
pEGFPC1/GgVcl 1-1066
 
Resource Report
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1+ mentions
RRID:Addgene_46265 Vcl Gallus gallus Kanamycin PMID:15728584 full length chicken vinculin (EcoRI fragment of p1005 subcloned into EcoRI-cut pEGFP-C1). ATG at bp 1390-1392. TAA at bp 4588-90. EGFP at N-terminus. Backbone Marker:Clonetech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:15:30 1
pET15b/EYFP-GgVcl 1-258
 
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RRID:Addgene_46263 Vcl 1-258 Gallus gallus Ampicillin PMID:15728584 EYFP was PCR amplified to introduce NdeI site at both 5' and 3' ends. The NdeI flanked EYFP was inserted into NdeI site of pET15b/Vh1-258 (Addgene plasmid 46173), where V1-258 was cloned between NdeI and XhoI. Plasmid contains a 12-residue spacer between the EYFP and vinculin domain. Backbone Marker:EMD; Backbone Size:5700; Vector Backbone:pET-15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin aa 1-258 (VD1) 2026-08-15 01:15:30 0
pcDNA3-FLAG-HA-CAD S1900A
 
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RRID:Addgene_46240 carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase Mus musculus Ampicillin PMID:23429703 Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin Serine 1900 changed to Alanine (S1900A) 2026-08-15 01:15:29 0
pTK93_Lifeact-mCherry
 
Resource Report
Resource Website
1+ mentions
RRID:Addgene_46357 Lifeact Saccharomyces cerevisiae Ampicillin PMID:23870127 Backbone Size:5000; Vector Backbone:pBABE; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin 2026-08-15 01:15:31 9
pTK88_GFP-Anillin
 
Resource Report
Resource Website
1+ mentions
RRID:Addgene_46354 Anillin Homo sapiens Ampicillin PMID:23870127 Backbone Size:6000; Vector Backbone:pBABE; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin a1854 is changed to c to disrupt XhoI site without affecting coding amino acid sequence 2026-08-15 01:15:31 1
pTK91_GFP-MRLC2
 
Resource Report
Resource Website
1+ mentions
RRID:Addgene_46355 MRLC2 Homo sapiens Ampicillin PMID:23870127 Backbone Size:6000; Vector Backbone:pBABE; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin 2026-08-15 01:15:31 2
pTK96_mCherry-MRLC2
 
Resource Report
Resource Website
1+ mentions
RRID:Addgene_46358 MRLC2 Homo sapiens Ampicillin PMID:23870127 Backbone Size:6000; Vector Backbone:pBABE; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin 2026-08-15 01:15:31 1
pcDNA3.1(+) Drp1 V2 S600D
 
Resource Report
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RRID:Addgene_46345 Drp1 V2 Mus musculus Ampicillin PMID:23283981 Backbone Marker:Invitrogen; Backbone Size:5378; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin S600D 2026-08-15 01:15:30 0

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