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Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:2905; Vector Backbone:pBluescript II; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25324299
Proper citation: RRID:Addgene_60950 Copy
Genetic Insert: tdTomato
Vector Backbone Description: Backbone Marker:GeneArt; Backbone Size:2450; Vector Backbone:pMXs; Vector Types:Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24503648
Proper citation: RRID:Addgene_60953 Copy
Species: Synthetic
Genetic Insert: SpCas9
Vector Backbone Description: Backbone Size:2905; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25326897
Proper citation: RRID:Addgene_60957 Copy
Genetic Insert: eGFP-Kana_I-SceI
Vector Backbone Description: Vector Backbone:pEGFP N1; Vector Types:; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:16526409
Proper citation: RRID:Addgene_60961 Copy
Species: Rattus norvegicus
Genetic Insert: Agouti signaling protein gRNA
Vector Backbone Description: Backbone Size:2147; Vector Backbone:DR274; Vector Types:CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24967838
Comments: Targets a 19-bp deletion in exon 2 of the Agouti signalling protein (Asip) gene
Proper citation: RRID:Addgene_60968 Copy
Species: Rattus norvegicus
Genetic Insert: Tyrosinase gRNA
Vector Backbone Description: Backbone Size:2147; Vector Backbone:DR274; Vector Types:CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24967838
Comments: Targets a mutation 896G>A in exon 2 of the Tyr gene resulting in an Arg299His missense mutation
Proper citation: RRID:Addgene_60967 Copy
Species: Rattus norvegicus
Genetic Insert: v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog gRNA
Vector Backbone Description: Backbone Size:2147; Vector Backbone:DR274; Vector Types:CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24967838
Comments: Targets an integrated 7,098-bp endogenous retroviral element (ERV) within the first intron of the Kit gene
Proper citation: RRID:Addgene_60970 Copy
Species: Synthetic
Genetic Insert: GFP-Luciferase fusion
Vector Backbone Description: Backbone Size:2877; Vector Backbone:pBluescript; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24755823
Proper citation: RRID:Addgene_60976 Copy
Species: Synthetic
Genetic Insert: ddRFP A-WW domain-ERK substrate-ddFP B
Vector Backbone Description: Backbone Size:5500; Vector Backbone:pcDNA3.0; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25622108
Proper citation: RRID:Addgene_60974 Copy
Species: Synthetic
Genetic Insert: mKate2
Vector Backbone Description: Vector Backbone:pMTB; Vector Types:Zebrafish; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26010570
Comments: Multibow constructs contain repetitive loxP sites that make these constructs prone to recombination. The constructs display a few issues with bacterial stability and sequencing that need to be considered when amplifying, storing and using them. First, in our hands Multibow constructs have displayed instability in E.coli cultures and had low yield in Maxi/Midi-preps. Mini-prep is recommended to harvest Multibow constructs. For transformation, we have had success with 5-alpha F'Iq cells (NEB). Second, the sequencing of Multibow constructs may run into problems of low quality/inaccurate reads. A problematic trace file does not necessarily mean the construct is wrong or the sample has mixed sequences. Third, before using the constructs harvested from mini-prep for injections, we recommend a further purification step using DNA purification kits such as MinElute PCR purification kit (Qiagen). All Multibow constructs share a backbone of pMTB vector (AMP resistance) containing the tol2 sites for transgenic insertion and the loxP recombination sites. To validate the variable region, we recommend sequencing with this specific primer: 5'-CAGCAGGACCATTTATCATGCTGCTGC-3', which recognizes the end of the variable region. The Sp6 primer may not provide enough reading length to reach the variable region.
Proper citation: RRID:Addgene_60984 Copy
Species: Homo sapiens
Genetic Insert: MYT1L
Vector Backbone Description: Backbone Marker:Homemade; Backbone Size:8465; Vector Backbone:N106; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25374357
Proper citation: RRID:Addgene_60861 Copy
Species: Synthetic
Genetic Insert: mKO
Vector Backbone Description: Vector Backbone:pMTB; Vector Types:Zebrafish; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26010570
Comments: Multibow constructs contain repetitive loxP sites that make these constructs prone to recombination. The constructs display a few issues with bacterial stability and sequencing that need to be considered when amplifying, storing and using them. First, in our hands Multibow constructs have displayed instability in E.coli cultures and had low yield in Maxi/Midi-preps. Mini-prep is recommended to harvest Multibow constructs. For transformation, we have had success with 5-alpha F'Iq cells (NEB). Second, the sequencing of Multibow constructs may run into problems of low quality/inaccurate reads. A problematic trace file does not necessarily mean the construct is wrong or the sample has mixed sequences. Third, before using the constructs harvested from mini-prep for injections, we recommend a further purification step using DNA purification kits such as MinElute PCR purification kit (Qiagen). All Multibow constructs share a backbone of pMTB vector (AMP resistance) containing the tol2 sites for transgenic insertion and the loxP recombination sites. To validate the variable region, we recommend sequencing with this specific primer: 5'-CAGCAGGACCATTTATCATGCTGCTGC-3', which recognizes the end of the variable region. The Sp6 primer may not provide enough reading length to reach the variable region.
Proper citation: RRID:Addgene_60982 Copy
Vector Backbone Description: Backbone Size:8506; Vector Backbone:pUC ori vector; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27030102
Comments: The gRNA cloning strategy is exactly the same as for pX330/pX335.
Proper citation: RRID:Addgene_60900 Copy
Species: Synthetic
Genetic Insert: citrine
Vector Backbone Description: Vector Backbone:pMTB; Vector Types:Zebrafish; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26010570
Comments: Multibow constructs contain repetitive loxP sites that make these constructs prone to recombination. The constructs display a few issues with bacterial stability and sequencing that need to be considered when amplifying, storing and using them. First, in our hands Multibow constructs have displayed instability in E.coli cultures and had low yield in Maxi/Midi-preps. Mini-prep is recommended to harvest Multibow constructs. For transformation, we have had success with 5-alpha F'Iq cells (NEB). Second, the sequencing of Multibow constructs may run into problems of low quality/inaccurate reads. A problematic trace file does not necessarily mean the construct is wrong or the sample has mixed sequences. Third, before using the constructs harvested from mini-prep for injections, we recommend a further purification step using DNA purification kits such as MinElute PCR purification kit (Qiagen). All Multibow constructs share a backbone of pMTB vector (AMP resistance) containing the tol2 sites for transgenic insertion and the loxP recombination sites. To validate the variable region, we recommend sequencing with this specific primer: 5'-CAGCAGGACCATTTATCATGCTGCTGC-3', which recognizes the end of the variable region. The Sp6 primer may not provide enough reading length to reach the variable region.
Proper citation: RRID:Addgene_60988 Copy
Species: Ruminococcus flavefaciens
Genetic Insert: CohIII
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5216; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25482395
Proper citation: RRID:Addgene_60866 Copy
Species: Synthetic
Genetic Insert: Xyl
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:4304; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25482395
Proper citation: RRID:Addgene_60865 Copy
Species: Synthetic
Genetic Insert: EBFP2
Vector Backbone Description: Vector Backbone:pMTB; Vector Types:Zebrafish; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26010570
Comments: Multibow constructs contain repetitive loxP sites that make these constructs prone to recombination. The constructs display a few issues with bacterial stability and sequencing that need to be considered when amplifying, storing and using them. First, in our hands Multibow constructs have displayed instability in E.coli cultures and had low yield in Maxi/Midi-preps. Mini-prep is recommended to harvest Multibow constructs. For transformation, we have had success with 5-alpha F'Iq cells (NEB). Second, the sequencing of Multibow constructs may run into problems of low quality/inaccurate reads. A problematic trace file does not necessarily mean the construct is wrong or the sample has mixed sequences. Third, before using the constructs harvested from mini-prep for injections, we recommend a further purification step using DNA purification kits such as MinElute PCR purification kit (Qiagen). All Multibow constructs share a backbone of pMTB vector (AMP resistance) containing the tol2 sites for transgenic insertion and the loxP recombination sites. To validate the variable region, we recommend sequencing with this specific primer: 5'-CAGCAGGACCATTTATCATGCTGCTGC-3', which recognizes the end of the variable region. The Sp6 primer may not provide enough reading length to reach the variable region.
Proper citation: RRID:Addgene_60985 Copy
Genetic Insert: scFv-GCN4
Vector Backbone Description: Vector Backbone:pHR; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25307933
Comments: For more information, visit https://valelab4.ucsf.edu/external/research/suntag.html
Note this plasmid was used with the CRISPRa library, as described in Gilbert et al. Cell 2014 PubMed ID: 25307932.
Proper citation: RRID:Addgene_60904 Copy
Genetic Insert: Cas9 dead
Vector Backbone Description: Vector Backbone:pHR; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25307933
Comments: For more information, visit https://valelab4.ucsf.edu/external/research/suntag.html
Note this plasmid was used with the CRISPRa library, as described in Gilbert et al. Cell 2014 PubMed ID: 25307932.
Proper citation: RRID:Addgene_60903 Copy
Species: Homo sapiens
Genetic Insert: 1802 bp promoter fragment of Cancerous Inhibitor of PP2A
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4242; Vector Backbone:pGL4.10(luc2); Vector Types:Luciferase, Promoterless; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21445343
Comments: Please note that there are some discrepancies between Addgene's quality control sequences and the depositor's sequence. The depositor noted that these discrepancies do NOT affect plasmid function.
Proper citation: RRID:Addgene_60868 Copy
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