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Species: E. Coli
Genetic Insert: LacI-T
Vector Backbone Description: Backbone Marker:Expressys; Backbone Size:4040; Vector Backbone:pZS5; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25035932
Proper citation: RRID:Addgene_60756 Copy
Species: Synthetic
Genetic Insert: single polypeptide FPX biosensor for caspase-1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA3 (+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25622108
Proper citation: RRID:Addgene_60885 Copy
Species: Synthetic
Genetic Insert: single polypeptide FPX biosensor for caspase-3
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA3 (+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25622108
Proper citation: RRID:Addgene_60883 Copy
Species: Homo sapiens
Genetic Insert: Tpr
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18794356
Proper citation: RRID:Addgene_60882 Copy
Species: Homo sapiens
Genetic Insert: Dihydrofolate reductase
Vector Backbone Description: Vector Backbone:pUG27; Vector Types:LoxP flanked deletion cassette template; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25688547
Comments: Dihydrofolate reductase gene from Homo sapiens carrying L22F and F31S mutations (DHFR*); codon optimized for expression in Saccharomyces cerevisiae. It also contains an additional F180L mutation that does not impact protein function.
Proper citation: RRID:Addgene_60929 Copy
Species: Homo sapiens
Genetic Insert: ErbB-3 promoter
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4818; Vector Backbone:pGL3-basic; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11859873
Proper citation: RRID:Addgene_60899 Copy
Species: E. coli
Genetic Insert: Cre
Vector Backbone Description: Vector Backbone:pSH47; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25688547
Comments: This plasmid contains I100V, K245R and F258L mutations in the ADE2 gene that do not impact selection or plasmid function.
Proper citation: RRID:Addgene_60932 Copy
Species: Aequorea victoria
Genetic Insert: Cerulean(159-238)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4800; Vector Backbone:pcDNA1/amp; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17596375
Comments: N164H substitution added during PCR of Cerulean(159-238).
Reference for mCerulean:
Rizzo MA, Springer GH, Granada B, Piston DW. An improved cyan fluorescent protein variant useful for FRET. Nat Biotechnol. 2004;22(4):445-9. PubMed PMID: 14990965.
Proper citation: RRID:Addgene_60898 Copy
Species: Aequorea victoria
Genetic Insert: Cerulean(1-158)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4800; Vector Backbone:pcDNA1/amp; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16641313
Comments: Reference for mCerulean:
Rizzo MA, Springer GH, Granada B, Piston DW. An improved cyan fluorescent protein variant useful for FRET. Nat Biotechnol. 2004;22(4):445-9. PubMed PMID: 14990965.
Proper citation: RRID:Addgene_60897 Copy
Species: E. coli
Genetic Insert: Cre
Vector Backbone Description: Vector Backbone:pSH47; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25688547
Proper citation: RRID:Addgene_60930 Copy
Species: Synthetic
Genetic Insert: PIP2 dimerization dependent sensor RA+B monomers
Vector Backbone Description: Vector Backbone:pUB2.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25622108
Comments: Contains a dimerization dependent fluorescent protein, originally described by Alford, S.C., Ding, Y., Simmen, T., and Campbell, R.E. (2012). Dimerization-Dependent Green and Yellow Fluorescent Proteins. ACS Synth. Biol. 1, 569–575.
Addgene NGS results identified a frameshift mutation in the HygR gene originally indicated in this plasmid. The depositing lab has confirmed that their experiments were done with transient transfection and did not make use of Hygromycin selection. For users who need a mammalian selection marker, they recommend moving the biosensor coding region from this plasmid into a different plasmid backbone.
Proper citation: RRID:Addgene_60937 Copy
Species: Synthetic
Genetic Insert: PKA FPX Sensor
Vector Backbone Description: Vector Backbone:pUB2.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25622108
Comments: Contains a dimerization dependent fluorescent protein, originally described by Alford, S.C., Ding, Y., Simmen, T., and Campbell, R.E. (2012). Dimerization-Dependent Green and Yellow Fluorescent Proteins. ACS Synth. Biol. 1, 569–575.
Proper citation: RRID:Addgene_60936 Copy
Species: Mus musculus
Genetic Insert: Ten-Eleven Translocation 2
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pcDNA3*; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24412366
Comments: *modified pcDNA3 vector with a beta globulin intron inserted. This insertion can help stabilize the transcripts and is particularly useful for the expression of large genes, such as p300 and Tet proteins
Proper citation: RRID:Addgene_60939 Copy
Species: Aequorea victoria
Genetic Insert: CFP(1-158)Stop
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4800; Vector Backbone:pcDNA1/amp; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_60892 Copy
Species: Aequorea victoria
Genetic Insert: YFP(159-238)Stop
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4800; Vector Backbone:pcDNA1/amp; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_60891 Copy
Species: Mus musculus
Genetic Insert: Ten-Eleven Translocation 3
Vector Backbone Description: Backbone Size:6000; Vector Backbone:pcDNA-flag; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24412366
Proper citation: RRID:Addgene_60940 Copy
Species: Other
Genetic Insert: dsRED
Vector Backbone Description: Backbone Marker:Thermo Scientific; Backbone Size:2974; Vector Backbone:pJET1.2; Vector Types:Unspecified; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25324299
Comments: The plasmid contains an IS4-like element that does not interfere with function as described in the associated publication.
Proper citation: RRID:Addgene_60944 Copy
Species: Homo sapiens
Genetic Insert: Calpain 2
Vector Backbone Description: Backbone Marker:sigma; Backbone Size:6310; Vector Backbone:p3xFlag-CMV-14; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24412366
Proper citation: RRID:Addgene_60942 Copy
Species: Other
Genetic Insert: vas-phiC31 integrase.NLS
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:2961; Vector Backbone:pBluescript II; Vector Types:RMCE; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25324299
Proper citation: RRID:Addgene_60948 Copy
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:2905; Vector Backbone:pBluescript II; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25324299
Proper citation: RRID:Addgene_60949 Copy
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