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Species: Synthetic
Genetic Insert: RITE cassette
Vector Backbone Description: Vector Backbone:unknown; Vector Types:RITE cassette; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23708297
Proper citation: RRID:Addgene_64759 Copy
Species: Synthetic
Genetic Insert: RITE cassette
Vector Backbone Description: Vector Backbone:unknown; Vector Types:RITE cassette; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23708297
Comments: The discrepancies between the depositor's sequence and Addgene’s sequences have no functional consequences.
Proper citation: RRID:Addgene_64758 Copy
Species: Synthetic
Genetic Insert: RITE cassette
Vector Backbone Description: Vector Backbone:unknown; Vector Types:RITE cassette; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20018668
Comments: The discrepancies between the depositor's sequence and Addgene’s sequences have no functional consequences.
Proper citation: RRID:Addgene_64755 Copy
Species: Synthetic
Genetic Insert: RITE cassette
Vector Backbone Description: Vector Backbone:unknown; Vector Types:RITE cassette; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23708297
Comments: The discrepancies between the depositor's sequence and Addgene's sequences have no functional consequences.
Proper citation: RRID:Addgene_64754 Copy
Species: Synthetic
Genetic Insert: RITE cassette
Vector Backbone Description: Vector Backbone:unknown; Vector Types:RITE cassette; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23708297
Comments: The discrepancies between the depositor's sequence and Addgene’s sequences have no functional consequences.
Proper citation: RRID:Addgene_64757 Copy
Species: Synthetic
Genetic Insert: RITE cassette
Vector Backbone Description: Vector Backbone:unknown; Vector Types:RITE cassette; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23708297
Proper citation: RRID:Addgene_64751 Copy
Species: Synthetic
Genetic Insert: RITE cassette
Vector Backbone Description: Vector Backbone:unknown; Vector Types:RITE cassette; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23708297
Comments: The discrepancies between the depositor's sequence and Addgene’s sequences have no functional consequences.
Proper citation: RRID:Addgene_64762 Copy
Species: Synthetic
Genetic Insert: RITE cassette
Vector Backbone Description: Vector Backbone:unknown; Vector Types:RITE cassette; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23708297
Proper citation: RRID:Addgene_64763 Copy
Species: Synthetic
Genetic Insert: RITE cassette
Vector Backbone Description: Vector Backbone:unknown; Vector Types:RITE cassette; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23708297
Proper citation: RRID:Addgene_64760 Copy
Species: Synthetic
Genetic Insert: pnGFP
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5968; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24059533
Comments: Please cite Z. Chen, W. Ren, Q.E. Wright and H-w. Ai*, J. Am. Chem. Soc., 2013, 135: 14940-14943.
Proper citation: RRID:Addgene_64913 Copy
Species: Synthetic
Genetic Insert: hsGFP
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5968; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25141269
Comments: Please cite Z. Chen and H-w. Ai*, Biochemistry, 2014, 53: 5966-5974; and S. Chen, Z. Chen, W. Ren and H-w. Ai*, J. Am. Chem. Soc., 2012, 134: 9589-9592. Please note that you will also need a pMAH plasmid for mammalian expression. pMAH-POLY (Z. Chen, W. Ren, Q.E. Wright and H-w. Ai*, J. Am. Chem. Soc., 2013, 135: 14940-14943.) is also deposited to Addgene, which can be used to replace pMAH-AzF for mammalian applications.
Proper citation: RRID:Addgene_64912 Copy
Species: Synthetic
Genetic Insert: hsGFP
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3954; Vector Backbone:pBAD/HisB; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25141269
Comments: Please cite: Z. Chen and H-w. Ai*, Biochemistry, 2014, 53: 5966-5974; and S. Chen, Z. Chen, W. Ren and H-w. Ai*, J. Am. Chem. Soc., 2012, 134: 9589-9592. For E. coli expression, you will also need pEVOL-pAzF (Addgene # 31186): https://www.addgene.org/31186/
Proper citation: RRID:Addgene_64911 Copy
Species: Synthetic
Genetic Insert: SEP
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3956; Vector Backbone:pCR4-TOPO; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:24403083
Comments: Note that the coding sequence for SEP is lacking a start codon.
Proper citation: RRID:Addgene_64944 Copy
Species: Synthetic
Genetic Insert: mRuby2
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25871405
Comments: Type 3 parts are generally reserved for coding sequences in the Dueber YTK. For more information, please visit the supplemental information in PMID:25871405.
Proper citation: RRID:Addgene_65141 Copy
Species: Synthetic
Genetic Insert: mTurquoise2
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25871405
Comments: Type 3a parts are used for N-terminal coding sequences. This plasmid encodes for an N-terminal fluorescent protein tag. For more information, please visit the supplemental information in PMID:25871405.
Proper citation: RRID:Addgene_65144 Copy
Species: Synthetic
Genetic Insert: MS2
Vector Backbone Description: Backbone Marker:NEB; Vector Backbone:pMal-c; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18570876
Comments: The following reference describes how to isolate RNA binding complexes using this construct:
Jurica and Moore, Methods. 2002 Nov;28(3):336-45. https://www.ncbi.nlm.nih.gov/pubmed/12431437
Proper citation: RRID:Addgene_65104 Copy
Species: Synthetic
Genetic Insert: ConL5
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25871405
Comments: Type 1 parts are 5' assembly connectors in the Dueber YTK. For more information, please visit the supplemental information in PMID:25871405.
Proper citation: RRID:Addgene_65114 Copy
Species: Synthetic
Genetic Insert: ConL1
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25871405
Comments: Type 1 parts are 5' assembly connectors in the Dueber YTK. For more information, please visit the supplemental information in PMID:25871405.
Proper citation: RRID:Addgene_65110 Copy
Species: Synthetic
Genetic Insert: ConL3
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25871405
Comments: Type 1 parts are 5' assembly connectors in the Dueber YTK. For more information, please visit the supplemental information in PMID:25871405.
Proper citation: RRID:Addgene_65112 Copy
Species: Synthetic
Genetic Insert: 6XHis_3XFlag
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25871405
Comments: Type 3a parts are used for N-terminal coding sequences. This plasmid encodes for an N-terminal protein purification tag. For more information, please visit the supplemental information in PMID:25871405.
Proper citation: RRID:Addgene_65147 Copy
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