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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
pJB073
 
Resource Report
Resource Website
RRID:Addgene_98912 ZapB E. coli Chloramphenicol PMID:25848771 Backbone Marker:NBRP-E.coli at NIG; Backbone Size:4500; Vector Backbone:pCA24N; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol 2026-08-15 01:22:41 0
pJB057
 
Resource Report
Resource Website
RRID:Addgene_98911 ZapA E. coli Chloramphenicol PMID:25848771 Backbone Marker:NBRP-E.coli at NIG; Backbone Size:4500; Vector Backbone:pCA24N; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol 2026-08-15 01:22:43 0
pJB007
 
Resource Report
Resource Website
RRID:Addgene_98995 FtsI E. coli Chloramphenicol PMID:28209899 Backbone Marker:NBRP-E.coli at NIG; Vector Backbone:pCA24N; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol 2026-08-15 01:22:42 0
pXY027
 
Resource Report
Resource Website
1+ mentions
RRID:Addgene_98915 FtsZ E. coli Chloramphenicol PMID:25848771 The His tag in N'-FtsZ was removed. 6bp were added between the RBS and start codon of FtsZ to decrease the expression level of FstZ-GFP. Vector Backbone:pCA24N; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol 2026-08-15 01:22:41 2
pRM006
 
Resource Report
Resource Website
RRID:Addgene_98922 FtsZ E. coli Ampicillin PMID:28209899 Vector Backbone:pTB146; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:22:41 0
pCH6 - pBait-ChiX
 
Resource Report
Resource Website
RRID:Addgene_174662 ChiX E. coli Spectinomycin PMID:32170306 Backbone Size:3900; Vector Backbone:pCDF and pBAD; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin 2026-08-15 01:22:57 0
L4440_BioBrick-SCR-sgRNA-B
 
Resource Report
Resource Website
1+ mentions
RRID:Addgene_177812 Scrambled sgRNA targeting sequences E. coli Ampicillin PMID:35636511 Vector Backbone:L4440_BioBrick-sgRNA; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:23:03 1
p416-GPD-HA-PPK(mutant)
 
Resource Report
Resource Website
RRID:Addgene_183944 PPK(mutant) E. coli Ampicillin PMID:35862758 Vector Backbone:p416; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin mutations in His435, His454, and His592 to Alanine 2026-08-15 01:23:04 0
pTS2401-Tier1-PhCMV-HygroR
 
Resource Report
Resource Website
RRID:Addgene_169493 PCMV-driven hph E. coli Ampicillin PMID:33857582 Backbone Size:4435; Vector Backbone:pMM1 (pcDNA3.1-based); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:23:09 0
pTS2365-Tier1-PhCMV-TetR
 
Resource Report
Resource Website
RRID:Addgene_169566 PCMV-driven tetracycline repressor protein class B from transposon Tn10 E. coli Ampicillin PMID:33857582 Backbone Size:4435; Vector Backbone:pMM1 (pcDNA3.1-based); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:23:12 0
psfaMs-Tn5
 
Resource Report
Resource Website
RRID:Addgene_183637 anti mouse secondary nanobody fused to Tn5 E. coli Kanamycin PMID:36536148 Please visit https://doi.org/10.1101/2022.03.08.483459 for bioRxiv preprint. Vector Backbone:pET-29b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:23:13 0
pBad-HPII
 
Resource Report
Resource Website
RRID:Addgene_105839 hydroperoxidase II catalase E. coli Ampicillin Please visit https://openlibrary-repo.ecampusontario.ca/jspui/handle/123456789/838 for Chemical Biology & Biochemistry Laboratory Using Genetic Code Expansion Manual. Backbone Marker:Invitrogen; Backbone Size:4100; Vector Backbone:pBad; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:00:55 0
pBad-HPII TAG348
 
Resource Report
Resource Website
RRID:Addgene_105844 hydroperoxidase II catalase E. coli Ampicillin Please visit https://openlibrary-repo.ecampusontario.ca/jspui/handle/123456789/838 for Chemical Biology & Biochemistry Laboratory Using Genetic Code Expansion Manual. Backbone Marker:Invitrogen; Backbone Size:4100; Vector Backbone:pBad; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin TAG stop codon at site 348 2026-08-15 01:00:56 0
pBad-HPII TAG568
 
Resource Report
Resource Website
RRID:Addgene_105845 hydroperoxidase II catalase E. coli Ampicillin Please visit https://openlibrary-repo.ecampusontario.ca/jspui/handle/123456789/838 for Chemical Biology & Biochemistry Laboratory Using Genetic Code Expansion Manual. Backbone Marker:Invitrogen; Backbone Size:4100; Vector Backbone:pBad; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin TAG stop codon at site 568 2026-08-15 01:00:56 0
pBad-HPII TAG283
 
Resource Report
Resource Website
RRID:Addgene_105843 hydroperoxidase II catalase E. coli Ampicillin Please visit https://openlibrary-repo.ecampusontario.ca/jspui/handle/123456789/838 for Chemical Biology & Biochemistry Laboratory Using Genetic Code Expansion Manual. Backbone Marker:Invitrogen; Backbone Size:4100; Vector Backbone:pBad; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin TAG stop codon at site 283 2026-08-15 01:01:00 0
pAT-9208
 
Resource Report
Resource Website
RRID:Addgene_124221 self-targeting gRNA E. coli Ampicillin PMID:33450024 Please note this plasmid contains Illumina 2nd step primer capture sites within the insert sequence that can affect next generation sequencing results. We suggest using sanger sequencing when working with this plasmid. Vector Backbone:pUC19; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Ampicillin 2026-08-15 01:04:04 0
pAT-9251
 
Resource Report
Resource Website
RRID:Addgene_124226 SpCas9-HF1 E. coli Chloramphenicol PMID:33450024 Vector Backbone:p15A; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Chloramphenicol 2026-08-15 01:04:04 0
3XFlag-pA-Tn5-Fl
 
Resource Report
Resource Website
10+ mentions
RRID:Addgene_124601 Protein A and hyperactive Tn5 transposase (Tnp) fusion protein E. coli Ampicillin PMID:31036827 Please note that verifying this plasmid by sequencing or digest is challenging due to the presence of the helper plasmid, as multiple bands or mispriming is likely to occur. See Addgene's sequencing results to compare sequences. This plasmid has been found to be somewhat unstable and prone to concatenation. Concatenation often does not impact plasmid function, but can reduce transformation or transfection efficiencies. If you have trouble isolating the monomeric version of this plasmid, you might consider linearizing, gel extracting, re-ligating, and transforming the plasmid. Backbone Size:8079; Vector Backbone:pTXB1-Tn5; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:04:08 32
PVS10
 
Resource Report
Resource Website
1+ mentions
RRID:Addgene_104398 rpoA-rpoB-rpoC-6xHis and rpoZ E. coli Ampicillin PMID:25665556 Plasmid pVS10 encodes the E. coli rpoA-rpoB-rpoC [His6] and rpoZ ORFs under control of T7 promoter. Backbone Marker:Novagen; Vector Backbone:pET; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:00:42 2
PIA900
 
Resource Report
Resource Website
1+ mentions
RRID:Addgene_104401 rpoA, B, C, and Z E. coli Ampicillin PMID:25665556 pIA900 (rpoA, B, C, and Z) encodes a C-terminally His-tagged β′ which can be removed using TEV protease. Linker with TEV-His10 cloned between NheI and StuI sites of pIA787; NheI site is gone Backbone Marker:Novagen; Vector Backbone:pET; Vector Types:Bacterial Expression, E. coli expression vector; Bacterial Resistance:Ampicillin 2026-08-15 01:00:42 3

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