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Species: Mus musculus
Genetic Insert: murine Syk tandem SH2 domains (Ser 8 to Gln 264), with interdomain A (Phe 119 to His 162) substituted by a flexible 20aa linker
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5422; Vector Backbone:pET-30a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26468009
Comments: encoding the following protein sequence: MSANHLTYFFGNITREEAEDYLVQGGMTDGLYLLRQSRNYLGGFALSVAHNRKAHHYTIERELNGTYAISGGRAHASPADLCHYHSQEPDGLICLLKKPFNRPPGVQPKTGPGGSGGSGGSGSGGSGGSGGSEKMPWFHGNISRDESEQTVLIGSKTNGKFLIRARDNSGSYALCLLHEGKVLHYRIDRDKTGKLSIPEGKKFDTLWQLVEHYSYKPDGLLRVLTVPCQKIGAQ
Proper citation: RRID:Addgene_111272 Copy
Species: Synthetic
Genetic Insert: ChETA-mRuby2
Vector Backbone Description: Backbone Size:4116; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29879392
Proper citation: RRID:Addgene_111389 Copy
Vector Backbone Description: Backbone Size:3154; Vector Backbone:pScaf; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30984875
Comments: Preprint available at https://www.biorxiv.org/content/early/2018/04/27/309682
Proper citation: RRID:Addgene_111401 Copy
Genetic Insert: pScaf-7560.1
Vector Backbone Description: Backbone Size:3154; Vector Backbone:pScaf; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30984875
Comments: Preprint available at https://www.biorxiv.org/content/early/2018/04/27/309682
Proper citation: RRID:Addgene_111406 Copy
Species: Homo sapiens
Genetic Insert: potassium voltage-gated channel subfamily KQT member 1 isoform 1 [Homo sapiens]
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29429937
Proper citation: RRID:Addgene_111452 Copy
Species: Synthetic
Genetic Insert: CaCas9/sgScADE2/stop-codon repair
Vector Backbone Description: Backbone Marker:RS Sikorski; Vector Backbone:pRS416; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29695624
Proper citation: RRID:Addgene_111444 Copy
Species: Mus musculus
Genetic Insert: mCAR
Vector Backbone Description: Backbone Size:4836; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29879392
Proper citation: RRID:Addgene_111393 Copy
Species: Homo sapiens
Genetic Insert: hCAR
Vector Backbone Description: Backbone Size:4117; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29879392
Proper citation: RRID:Addgene_111397 Copy
Species: Synthetic
Genetic Insert: CaCas9/sgRNA-BsmBI stuffer
Vector Backbone Description: Backbone Marker:RS Sikorski; Vector Backbone:pRS416; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29695624
Proper citation: RRID:Addgene_111436 Copy
Species: Synthetic
Genetic Insert: CaCas9/sgRNA-BsmBI stuffer
Vector Backbone Description: Backbone Marker:RS Sikorski; Vector Backbone:pRS416; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29695624
Proper citation: RRID:Addgene_111435 Copy
Species: Rattus norvegicus
Genetic Insert: PKC zeta
Vector Backbone Description: Backbone Size:4650; Vector Backbone:pCMV5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9768361
Proper citation: RRID:Addgene_10801 Copy
Species: Acidaminococcus sp. BV3L6
Genetic Insert: human codon optimized enAsCas12a-HF1 (E174R/N282A/S542R/K548R)
Vector Backbone Description: Backbone Marker:Connie Cepko lab (Addgene plasmid #11179); Vector Backbone:pCAG-CFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30742127
Proper citation: RRID:Addgene_107942 Copy
Species: Acidaminococcus sp. BV3L6
Genetic Insert: human codon optimized enAsCas12a (E174R/S542R/K548R)
Vector Backbone Description: Backbone Marker:Connie Cepko lab (Addgene plasmid #11179); Vector Backbone:pCAG-CFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30742127
Proper citation: RRID:Addgene_107941 Copy
Species: Acidaminococcus sp. BV3L6
Genetic Insert: human codon optimized DNase-inactive (D908A) enAsCas12a (E174R/S542R/K548R) fused to VPR activation domain
Vector Backbone Description: Backbone Marker:Connie Cepko lab (Addgene plasmid #11179); Vector Backbone:pCAG-CFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30742127
Proper citation: RRID:Addgene_107943 Copy
Species: Mus musculus
Genetic Insert: Egr2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5427; Vector Backbone:pcDNA3.1(-); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25535333
Comments: A Sal I site was introduced before the HindIII site to further transfer to pBabe.
Proper citation: RRID:Addgene_107997 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: gRNA-CAN1.Y, gRNA-ADE2.Y
Vector Backbone Description: Backbone Marker:George Church (Addgene plasmid # 43803); Backbone Size:5660; Vector Backbone:p426-SNR52p-gRNA.CAN1.Y-SUP4t; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25743786
Proper citation: RRID:Addgene_107929 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: gRNA-CAN1.Y, gRNA-ADE2.Y
Vector Backbone Description: Backbone Marker:George Church (Addgene plasmid # 43803); Backbone Size:5817; Vector Backbone:p426-SNR52p-gRNA.CAN1.Y-SUP4t; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25743786
Proper citation: RRID:Addgene_107927 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: gRNA-CAN1.Y, gRNA-ADE2.Y
Vector Backbone Description: Backbone Marker:George Church (Addgene plasmid # 43803); Backbone Size:5577; Vector Backbone:p426-SNR52p-gRNA.CAN1.Y-SUP4t; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25743786
Proper citation: RRID:Addgene_107924 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: gRNA-CAN1.Y
Vector Backbone Description: Backbone Marker:George Church (Addgene plasmid # 43803); Backbone Size:5593; Vector Backbone:p426-SNR52p-gRNA.CAN1.Y-SUP4t; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25743786
Proper citation: RRID:Addgene_107923 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: gRNA-CAN1.Y
Vector Backbone Description: Backbone Marker:George Church (Addgene plasmid # 43803); Backbone Size:5775; Vector Backbone:p426-SNR52p-gRNA.CAN1.Y-SUP4t; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25743786
Proper citation: RRID:Addgene_107922 Copy
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