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Species: Synthetic
Genetic Insert: cymR repressor and PCymRC cumic acid-inducible promoter
Vector Backbone Description: Backbone Marker:iGEM; Vector Backbone:pSB1C3; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Proper citation: RRID:Addgene_204071 Copy
Species: Synthetic
Genetic Insert: L3S2P21 strong terminator
Vector Backbone Description: Backbone Marker:iGEM; Vector Backbone:pSB1C3; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Proper citation: RRID:Addgene_204116 Copy
Species: Synthetic
Genetic Insert: vanR repressor and PVanCC vanillic acid-inducible promoter
Vector Backbone Description: Backbone Marker:iGEM; Vector Backbone:pSB1C3; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Proper citation: RRID:Addgene_204078 Copy
Species: Synthetic
Genetic Insert: T7 terminator
Vector Backbone Description: Backbone Marker:iGEM; Vector Backbone:pSB1C3; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Proper citation: RRID:Addgene_204124 Copy
Species: Synthetic
Genetic Insert: MCD1 strong RBS
Vector Backbone Description: Backbone Marker:iGEM; Vector Backbone:pSB1C3; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Proper citation: RRID:Addgene_204091 Copy
Species: Synthetic
Genetic Insert: MCD7 medium RBS
Vector Backbone Description: Backbone Marker:iGEM; Vector Backbone:pSB1C3; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Proper citation: RRID:Addgene_204094 Copy
Vector Backbone Description: Vector Backbone:see comments; Vector Types:; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:8412673
Comments: MCS: EcoRV-BglII-XhoI-BssHII-SpeI-BsiWI-AvrII-AflII-BstBI and potentially other sites
sacB was derived from pUM24 (Ried, J. L., and A. Colimer. Gene 57:239-246, 1987) and from pUCD800 (Gay, et al. 1985 J. Bacteriol. 164:918-921). The polylinker came, in large part, from the plC20R polylinker (Marsh et al.. 1984: Gene 32: 481-485) and a polylinker region of plasmid pJRD184 (Heusterspreute et al.. 1985: Gene 37: 299-304).
The erythromycin-resistance gene came from Horinouchi, S., and Weisblum, B. (J. Bacteriol. 150: 804-814, 1982).
Proper citation: RRID:Addgene_70688 Copy
Species: S. aureus
Genetic Insert: mammalian codon-optimized Staphylococcus aureus dCas9
Vector Backbone Description: Backbone Marker:EMD Millipore; Vector Backbone:pACYCDuet-1; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26524662
Comments: For this plasmid, the sequence of the sgRNA spacer is GGGCACGGGCAGCTTGCCGG. The length of the sgRNA (not including the spacer sequence) is 84 nucleotides, where this truncated repeat/anti-repeat sgRNA is similar to that described previously by Ran et al., Nature 2015 (PubMed ID: 25830891) and conveys higher activity than the canonical full length sgRNA.
Proper citation: RRID:Addgene_70703 Copy
Species: S. aureus
Genetic Insert: mammalian codon-optimized Staphylococcus aureus Cas9
Vector Backbone Description: Backbone Marker:EMD Millipore; Vector Backbone:pACYCDuet-1; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26524662
Comments: For this plasmid, the sequence of the sgRNA spacer is GGGCACGGGCAGCTTGCCGG. The length of the sgRNA (not including the spacer sequence) is 84 nucleotides, where this truncated repeat/anti-repeat sgRNA is similar to that described previously by Ran et al., Nature 2015 (PubMed ID: 25830891) and conveys higher activity than the canonical full length sgRNA.
Proper citation: RRID:Addgene_70702 Copy
Species: S. aureus
Genetic Insert: mammalian codon-optimized Staphylococcus aureus Cas9
Vector Backbone Description: Backbone Marker:EMD Millipore; Vector Backbone:pACYCDuet-1; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26524662
Comments: For this plasmid, the sequence of the sgRNA spacer is GTCGCCCTCGAACTTCACCT. The length of the sgRNA (not including the spacer sequence) is 84 nucleotides, where this truncated repeat/anti-repeat sgRNA is similar to that described previously by Ran et al., Nature 2015 (PubMed ID: 25830891) and conveys higher activity than the canonical full length sgRNA.
Proper citation: RRID:Addgene_70705 Copy
Species: Synthetic
Genetic Insert: AD1 Sense
Vector Backbone Description: Backbone Marker:N/A; Vector Backbone:Custom; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25643173
Proper citation: RRID:Addgene_71207 Copy
Genetic Insert: pbuE Sense
Vector Backbone Description: Backbone Marker:N/A; Vector Backbone:Custom; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25643173
Proper citation: RRID:Addgene_71209 Copy
Vector Backbone Description: Backbone Size:4378; Vector Backbone:pGK12; Vector Types:Bacterial Expression, E. coli-gram positive bacteria shuttle vector; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:11526048
Comments: In plasmid preparations from E. coli, a smaller deletion derivative of pTRK669 of about 1.7 kb may be noticeable. This smaller plasmid does not affect the functionality of pTRK669 in gram positive hosts and may not appear in those backgrounds. Addgene's quality control sequence shows the expected deletion from the smaller derivative of pTRK669.
Whole plasmid sequencing from the depositor showed deletion of the NheI site. Please see the provided digest result for reference.
Proper citation: RRID:Addgene_71313 Copy
Genetic Insert: none
Vector Backbone Description: Vector Backbone:N/A; Vector Types:; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25769287
Proper citation: RRID:Addgene_71420 Copy
Genetic Insert: none
Vector Backbone Description: Vector Backbone:N/A; Vector Types:; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25769287
Proper citation: RRID:Addgene_71421 Copy
Species: S. pyogenes
Genetic Insert: Cas9
Vector Backbone Description: Vector Backbone:pSC101ts; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26141150
Comments: The plasmid curing system consists of an arabinose inducible promoter, PBAD to express a gRNA targeting the bla gene on the gRNA plasmid. When induced, the bla-targeting gRNA is expressed, leading Cas9 to cleave the gRNA plasmid and resulting in plasmid elimination.
Proper citation: RRID:Addgene_71538 Copy
Genetic Insert: loxP-hyg-cat-loxP
Vector Backbone Description: Backbone Marker:Jeffery Murry - Eric Rubin Lab; Vector Backbone:pJM1; Vector Types:Bacterial Expression, Source of loxP-hyg-cat- loxP for PCR amplification.; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25779316
Comments: This vector contains a hyg-cat cassette, flanked by loxP sites, that can be used for the construction of recombineeng substrates for M. smegmatis or M. tuberculosis. It contains a 1 bp change (relative to starting plasmid pJM1) at position 2008 (G to C) that eliminate a potential stop codon that could interfere with expression of downstream functions following excision of the cassette from the chromosome by Cre recombinase.
The hygromycin resistance gene encodes M13I and I111T variants compared to the NCBI reference [ADL66925.1]. These variants should not interfere with function.
Proper citation: RRID:Addgene_71487 Copy
Vector Backbone Description: Backbone Size:3865; Vector Backbone:pG1C; Vector Types:Bacterial Expression, Synthetic Biology, Shuttle Vector; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:27332993
Comments: Plasmid from the Geobacillus plasmid set - a modular shuttle vector toolkit for thermophile metabolic engineering
Proper citation: RRID:Addgene_71740 Copy
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Insect Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:30541104
Comments: Please note that the full plasmid sequence contains a few differences compared to GenBank ID: KM977569. These differences do not affect the function of the plasmid.
Proper citation: RRID:Addgene_71764 Copy
Species: Staphylococcus aureus ATCC 10832
Genetic Insert: sortase A
Vector Backbone Description: Backbone Marker:IBA; Backbone Size:3211; Vector Backbone:pASK-IBA3 plus; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25864513
Proper citation: RRID:Addgene_64983 Copy
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