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Species: Homo sapiens
Genetic Insert: scFv-Fc-fusion specific for Notch2 (clone B9)
Vector Backbone Description: Backbone Marker:McCafferty lab unpublished; Vector Backbone:pBIOCAM5-3F; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22842086
Comments: Binds to both mouse mNRR2 and human hNRR2. but neither NRR1.
Proper citation: RRID:Addgene_39348 Copy
Species: Homo sapiens
Genetic Insert: ERK1
Vector Backbone Description: Vector Backbone:NpT7-5; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8444886
Proper citation: RRID:Addgene_39229 Copy
Species: Homo sapiens
Genetic Insert: HERC2 HECT Domain
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21193487
Comments: HECT domain active site point mutation (C4762A substitution)
Proper citation: RRID:Addgene_39228 Copy
Species: Schizosaccharomyces pombe
Genetic Insert: urg1 promoter
Vector Backbone Description: Backbone Marker:Addgene plasmid # 39280; Backbone Size:2300; Vector Backbone:pFA6a-kanMX6-P3nmt1; Vector Types:Yeast genomic targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18197241
Proper citation: RRID:Addgene_39349 Copy
Species: Homo sapiens
Genetic Insert: scFv-Fc-fusion specific for Notch1 (clone B9)
Vector Backbone Description: Backbone Marker:McCafferty lab unpublished; Vector Backbone:pBIOCAM5-3F; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22842086
Comments: Binds to both mouse mNRR1 and human hNRR1 but not NRR2.
Proper citation: RRID:Addgene_39344 Copy
Species: Homo sapiens
Genetic Insert: scFv-Fc-fusion specific for Desmin (clone D7)
Vector Backbone Description: Backbone Marker:McCafferty lab unpublished; Vector Backbone:pBIOCAM5-3F; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18047641
Proper citation: RRID:Addgene_39346 Copy
Species: Homo sapiens
Genetic Insert: scFv specific for Jagged (clone D5)
Vector Backbone Description: Vector Backbone:pBIOCAM5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18047641
Proper citation: RRID:Addgene_39345 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Elongation factor 1 alpha-like protein
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5369; Vector Backbone:pET28b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_39337 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Elongation factor 1 alpha-like protein
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5369; Vector Backbone:pET28b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_39336 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Elongation factor 1 alpha-like protein
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5369; Vector Backbone:pET28b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_39338 Copy
Species: Helicobacter pylori
Genetic Insert: oxidoreductase
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5369; Vector Backbone:pET28b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: This construct can be expressed in BL21(DE3) cells.
Proper citation: RRID:Addgene_39334 Copy
Genetic Insert: KanR
Vector Backbone Description: Backbone Size:2300; Vector Backbone:pFA6a; Vector Types:Yeast genomic targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9717240
Proper citation: RRID:Addgene_39296 Copy
Species: Gallus gallus
Genetic Insert: Vcl 884-1066
Vector Backbone Description: Backbone Marker:EMD; Backbone Size:5700; Vector Backbone:pET-15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: CFP subcloned into 15b/V884-1066 (Addgene plasmid 46176) via a PCR strategy which placed Nde sites at the ends of the CFP cDNA and added the spacer region in EGFPC1/V1-1066 at the Cterminus of CFP.
Proper citation: RRID:Addgene_46180 Copy
Species: Shigella flexneri
Genetic Insert: IpaA
Vector Backbone Description: Backbone Marker:EMD; Backbone Size:5700; Vector Backbone:pET-15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15883197
Comments: IpaA cDNA was amplified from pEC14, supplied by M. Venketesan (Walter Reed Army Institute of Research, Washington, DC),
using appropriate primers to introduce an NdeI site before the initiating ATG and an XhoI site downstream of the stop codon. The
PCR product was transformed into TOPO pCRII (Invitrogen). The NdeI and XhoI fragment of IpaA cDNA was isolated from a pCRII clone containing the correct sequence and inserted into pET15b vector (Novogen) to generate pET15b/IpaA.
Proper citation: RRID:Addgene_46178 Copy
Species: Homo sapiens
Genetic Insert: PSD95.FingR
Vector Backbone Description: Backbone Size:5541; Vector Backbone:pGW_YFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23791193
Proper citation: RRID:Addgene_46299 Copy
Species: Gallus gallus
Genetic Insert: Vcl 884-1066
Vector Backbone Description: Backbone Marker:EMD; Backbone Size:5700; Vector Backbone:pET-15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_46179 Copy
Species: Homo sapiens
Genetic Insert: Nprl2
Vector Backbone Description: Vector Backbone:pLJM1; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23723238
Proper citation: RRID:Addgene_46338 Copy
Species: Homo sapiens
Genetic Insert: Depdc5
Vector Backbone Description: Vector Backbone:pLJM1; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23723238
Proper citation: RRID:Addgene_46336 Copy
Species: Gallus gallus
Genetic Insert: Vcl 1-851
Vector Backbone Description: Backbone Marker:EMD; Backbone Size:5700; Vector Backbone:pET-15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15728584
Comments: Using in-frame primers containing 5′-NdeI and 3′-XhoI sites, appropriate regions of the chicken vinculin cDNA sequence were amplified by PCR followed by addition of 3′-adenosine overhangs with Taq polymerase. The PCR products were ligated into the TOPO II plasmid (Invitrogen) and then subcloned into pET15b His tag expression vector (Novagen, Madison, WI) using NdeI and XhoI digestion of the multiple cloning site.
Proper citation: RRID:Addgene_46172 Copy
Species: Gallus gallus
Genetic Insert: Vcl 1-258
Vector Backbone Description: Backbone Marker:EMD; Backbone Size:5700; Vector Backbone:pET-15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15728584
Comments: Using in-frame primers containing 5′-NdeI and 3′-XhoI sites, appropriate regions of the chicken vinculin cDNA sequence were amplified by PCR followed by addition of 3′-adenosine overhangs with Taq polymerase. The PCR products were ligated into the TOPO II plasmid (Invitrogen) and then subcloned into pET15b His tag expression vector (Novagen, Madison, WI) using NdeI and XhoI digestion of the multiple cloning site.
Proper citation: RRID:Addgene_46173 Copy
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