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pHL-H1-ccdB-mEF1a-RiH
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_60601 Red Fluorescent Protein Discosoma sp Ampicillin PMID:25434822 Backbone Marker:Hotta Lab, CiRA; Backbone Size:2043; Vector Backbone:pHL-MCS1; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin 2026-08-15 01:17:34 9
pReporter_7
 
Resource Report
Resource Website 		RRID:Addgene_60567 Reporter_7 Synthetic Chloramphenicol PMID:25344638 This reporter plasmid contains the attB and attP sites flanking a green fluorescent protein reporter gene (gfp). A strong constitutive promoter (BBa_J23119) is placed upstream of the attB site, which transcribes in the opposite orientation as gfp. After the integrase is expressed, the orientation of gfp is inverted and it is transcribed. After inversion, recombined attB and attP sites result in the formation of attL and attR. The attL site is located on the 5'UTR and could impact gfp expression. To insulate against this effect, we included a spacer and the ribozyme RiboJ. Also contains mRFP in order to aid the gating of cells. Vector Backbone:unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol 2026-08-15 01:17:33 0
pHL-EF1a-SphcCas9(D10A)-iP-A
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_60600 CRISPR Cas9 D10A Streptococcus pyogenes Ampicillin PMID:25434822 Backbone Marker:Hotta Lab, CiRA; Backbone Size:6923; Vector Backbone:pHL-EF1a-GW-iP-A; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin Changed Asp 10 to Ala, Codon usage optimized for human usage 2026-08-15 01:17:34 2
pENTR-DMD-Donor
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_60605 Hygromycin resistant gene Synthetic Kanamycin PMID:25434822 Backbone Marker:Invitrogen; Backbone Size:3755; Vector Backbone:pENTR2B; Vector Types:Mammalian Expression, Knock-in donor vector; Bacterial Resistance:Kanamycin 2026-08-15 01:17:34 3
pENTR-D-TOPO-PAmCherry1-MCS
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_60608 PAmCherry1 Kanamycin PMID:24158481 This plasmid was used in Nickerson et al., 2014 (PMID: 24963703) for generation of PAmCherry1 fragments (addgene.org/60545 and addgene.org/60546) for BiFC-PALM. Backbone Marker:Invitrogen; Backbone Size:2580; Vector Backbone:pENTR/D-TOPO; Vector Types:Cloning; Bacterial Resistance:Kanamycin 2026-08-15 01:17:34 1
pIntegrase_5
 
Resource Report
Resource Website 		RRID:Addgene_60577 Integrase_5 Streptomyces phage PhiK38-1 Kanamycin PMID:25344638 Vector Backbone:unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin Codon optimized for E.coli 2026-08-15 01:17:33 0
pIntegrase_4
 
Resource Report
Resource Website 		RRID:Addgene_60576 Integrase_4 Streptococcus equi subsp. equi 4047 Kanamycin PMID:25344638 Vector Backbone:unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin Codon optimized for E.coli 2026-08-15 01:17:33 0
pIntegrase_2
 
Resource Report
Resource Website 		RRID:Addgene_60574 Integrase_2 Streptomyces scabiei 87.22 Kanamycin PMID:25344638 Vector Backbone:unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin Codon optimized for E.coli 2026-08-15 01:17:33 0
pReporter_12
 
Resource Report
Resource Website 		RRID:Addgene_60572 Reporter_12 Synthetic Chloramphenicol PMID:25344638 This reporter plasmid contains the attB and attP sites flanking a green fluorescent protein reporter gene (gfp). A strong constitutive promoter (BBa_J23119) is placed upstream of the attB site, which transcribes in the opposite orientation as gfp. After the integrase is expressed, the orientation of gfp is inverted and it is transcribed. After inversion, recombined attB and attP sites result in the formation of attL and attR. The attL site is located on the 5'UTR and could impact gfp expression. To insulate against this effect, we included a spacer and the ribozyme RiboJ. Also contains mRFP in order to aid the gating of cells. Vector Backbone:unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol 2026-08-15 01:17:33 0
pSBtet-RH
 
Resource Report
Resource Website 		RRID:Addgene_60500 Ampicillin PMID:25650551 Backbone Size:2021; Vector Backbone:pUC19; Vector Types:Mammalian Expression, Transposon; Bacterial Resistance:Ampicillin 2026-08-15 01:17:32 0
pCis_7+8+10
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_60588 Cis_7+8+10 Synthetic Kanamycin PMID:25344638 Vector Backbone:unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:17:33 1
pCis_7+8
 
Resource Report
Resource Website 		RRID:Addgene_60587 Cis_7+8 Synthetic Kanamycin PMID:25344638 Vector Backbone:unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:17:34 0
pCis_2+5
 
Resource Report
Resource Website 		RRID:Addgene_60586 Cis_2+5 Synthetic Kanamycin PMID:25344638 Vector Backbone:unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:17:33 0
pMemoryArray
 
Resource Report
Resource Website 		RRID:Addgene_60585 MemoryArray Synthetic Chloramphenicol PMID:25344638 An array was constructed by concatenating the attB/P sites of the 11 phage integrases to form a linear 2kb piece of DNA. Random spacers (50 bp) with 50% GC content are included between the att sites. Vector Backbone:unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol 2026-08-15 01:17:33 0
pSBtet-GB
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_60504 Ampicillin PMID:25650551 Backbone Size:2021; Vector Backbone:pUC19; Vector Types:Mammalian Expression, Transposon; Bacterial Resistance:Ampicillin 2026-08-15 01:17:33 2
pCis_2+7+8+5
 
Resource Report
Resource Website 		RRID:Addgene_60589 Cis_2+7+8+5 Synthetic Kanamycin PMID:25344638 Vector Backbone:unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:17:33 0
pSBtet-Hyg
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_60508 Ampicillin PMID:25650551 Backbone Size:2021; Vector Backbone:pUC19; Vector Types:Mammalian Expression, Transposon; Bacterial Resistance:Ampicillin 2026-08-15 01:17:33 9
pSBtet-Pur
 
Resource Report
Resource Website
10+ mentions 		RRID:Addgene_60507 Ampicillin PMID:25650551 Backbone Size:2021; Vector Backbone:pUC19; Vector Types:Mammalian Expression, Transposon; Bacterial Resistance:Ampicillin 2026-08-15 01:17:33 27
pSBtet-Neo
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_60509 Ampicillin PMID:25650551 Backbone Size:2021; Vector Backbone:pUC19; Vector Types:Mammalian Expression, Transposon; Bacterial Resistance:Ampicillin 2026-08-15 01:17:33 5
pIntegrase_9
 
Resource Report
Resource Website 		RRID:Addgene_60580 Integrase_9 Other Kanamycin PMID:25344638 Vector Backbone:unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin Codon optimized for E.coli 2026-08-15 01:17:33 0

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