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Species: Discosoma sp
Genetic Insert: Red Fluorescent Protein
Vector Backbone Description: Backbone Marker:Hotta Lab, CiRA; Backbone Size:2043; Vector Backbone:pHL-MCS1; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25434822
Proper citation: RRID:Addgene_60601 Copy
Species: Synthetic
Genetic Insert: Reporter_7
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25344638
Comments: This reporter plasmid contains the attB and attP sites flanking a green fluorescent protein reporter gene (gfp). A strong constitutive promoter (BBa_J23119) is placed upstream of the attB site, which transcribes in the opposite orientation as gfp. After the integrase is expressed, the orientation of gfp is inverted and it is transcribed. After inversion, recombined attB and attP sites result in the formation of attL and attR. The attL site is located on the 5'UTR and could impact gfp expression. To insulate against this effect, we included a spacer and the ribozyme RiboJ.
Also contains mRFP in order to aid the gating of cells.
Proper citation: RRID:Addgene_60567 Copy
Species: Streptococcus pyogenes
Genetic Insert: CRISPR Cas9 D10A
Vector Backbone Description: Backbone Marker:Hotta Lab, CiRA; Backbone Size:6923; Vector Backbone:pHL-EF1a-GW-iP-A; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25434822
Proper citation: RRID:Addgene_60600 Copy
Species: Synthetic
Genetic Insert: Hygromycin resistant gene
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3755; Vector Backbone:pENTR2B; Vector Types:Mammalian Expression, Knock-in donor vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25434822
Proper citation: RRID:Addgene_60605 Copy
Genetic Insert: PAmCherry1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2580; Vector Backbone:pENTR/D-TOPO; Vector Types:Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24158481
Comments: This plasmid was used in Nickerson et al., 2014 (PMID: 24963703) for generation of PAmCherry1 fragments (addgene.org/60545 and addgene.org/60546) for BiFC-PALM.
Proper citation: RRID:Addgene_60608 Copy
Species: Streptomyces phage PhiK38-1
Genetic Insert: Integrase_5
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25344638
Proper citation: RRID:Addgene_60577 Copy
Species: Streptococcus equi subsp. equi 4047
Genetic Insert: Integrase_4
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25344638
Proper citation: RRID:Addgene_60576 Copy
Species: Streptomyces scabiei 87.22
Genetic Insert: Integrase_2
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25344638
Proper citation: RRID:Addgene_60574 Copy
Species: Synthetic
Genetic Insert: Reporter_12
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25344638
Comments: This reporter plasmid contains the attB and attP sites flanking a green fluorescent protein reporter gene (gfp). A strong constitutive promoter (BBa_J23119) is placed upstream of the attB site, which transcribes in the opposite orientation as gfp. After the integrase is expressed, the orientation of gfp is inverted and it is transcribed. After inversion, recombined attB and attP sites result in the formation of attL and attR. The attL site is located on the 5'UTR and could impact gfp expression. To insulate against this effect, we included a spacer and the ribozyme RiboJ.
Also contains mRFP in order to aid the gating of cells.
Proper citation: RRID:Addgene_60572 Copy
Vector Backbone Description: Backbone Size:2021; Vector Backbone:pUC19; Vector Types:Mammalian Expression, Transposon; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25650551
Proper citation: RRID:Addgene_60500 Copy
Species: Synthetic
Genetic Insert: Cis_7+8+10
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25344638
Proper citation: RRID:Addgene_60588 Copy
Species: Synthetic
Genetic Insert: Cis_7+8
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25344638
Proper citation: RRID:Addgene_60587 Copy
Species: Synthetic
Genetic Insert: Cis_2+5
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25344638
Proper citation: RRID:Addgene_60586 Copy
Species: Synthetic
Genetic Insert: MemoryArray
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25344638
Comments: An array was constructed by concatenating the attB/P sites of the 11 phage integrases to form a linear 2kb piece of DNA. Random spacers (50 bp) with 50% GC content are included between the att sites.
Proper citation: RRID:Addgene_60585 Copy
Vector Backbone Description: Backbone Size:2021; Vector Backbone:pUC19; Vector Types:Mammalian Expression, Transposon; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25650551
Proper citation: RRID:Addgene_60504 Copy
Species: Synthetic
Genetic Insert: Cis_2+7+8+5
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25344638
Proper citation: RRID:Addgene_60589 Copy
Vector Backbone Description: Backbone Size:2021; Vector Backbone:pUC19; Vector Types:Mammalian Expression, Transposon; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25650551
Proper citation: RRID:Addgene_60508 Copy
Vector Backbone Description: Backbone Size:2021; Vector Backbone:pUC19; Vector Types:Mammalian Expression, Transposon; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25650551
Proper citation: RRID:Addgene_60507 Copy
Vector Backbone Description: Backbone Size:2021; Vector Backbone:pUC19; Vector Types:Mammalian Expression, Transposon; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25650551
Proper citation: RRID:Addgene_60509 Copy
Species: Other
Genetic Insert: Integrase_9
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25344638
Proper citation: RRID:Addgene_60580 Copy
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