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Species: Synthetic
Genetic Insert: GFPpA
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBS KS+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24172903
Comments: This construct is modified pGOF18 from Young Young Il Yeom et al.,Development 122, 000-000 (1996) 881.
Proper citation: RRID:Addgene_60527 Copy
Species: Homo sapiens
Genetic Insert: Pak5
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:23836671
Proper citation: RRID:Addgene_60533 Copy
Vector Backbone Description: Backbone Size:2021; Vector Backbone:pUC19; Vector Types:Mammalian Expression, Transposon; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25650551
Proper citation: RRID:Addgene_60499 Copy
Species: Homo sapiens
Genetic Insert: Pak5
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:23836671
Comments: This plasmid is derived from Addgene plasmid #23850. We changed a1208c, g79c to align with pubmed sequence and added a stop codon.
Proper citation: RRID:Addgene_60532 Copy
Vector Backbone Description: Backbone Size:2021; Vector Backbone:pUC19; Vector Types:Mammalian Expression, Transposon; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25650551
Proper citation: RRID:Addgene_60498 Copy
Species: Homo sapiens
Genetic Insert: FGFR4
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2546; Vector Backbone:pENTR221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23836671
Proper citation: RRID:Addgene_60531 Copy
Species: Homo sapiens
Genetic Insert: Pak5
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:23836671
Proper citation: RRID:Addgene_60535 Copy
Species: Homo sapiens
Genetic Insert: Pak5
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:23836671
Proper citation: RRID:Addgene_60539 Copy
Species: Homo sapiens
Genetic Insert: Pak5
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:23836671
Proper citation: RRID:Addgene_60538 Copy
Genetic Insert: mCherry
Vector Backbone Description: Backbone Size:5048; Vector Backbone:AAV2; Vector Types:AAV, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24768300
Proper citation: RRID:Addgene_60544 Copy
Species: Homo sapiens
Genetic Insert: ProtoSpacer AAVS1-T2
Vector Backbone Description: Backbone Marker:ClonTech; Backbone Size:4685; Vector Backbone:pEGFP C1; Vector Types:Mammalian Expression, CRISPR, Kan; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25193712
Proper citation: RRID:Addgene_60543 Copy
Genetic Insert: PAmCherry1 N-terminal fragment
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2580; Vector Backbone:pENTR/D-TOPO; Vector Types:Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24963703
Comments: Parent plasmid (full-length PAmCherry1 in pENTR) (Addgene plasmid 60608) originally reported in Nan et al., 2013 (PMID:24158481).
Proper citation: RRID:Addgene_60545 Copy
Species: Arabidopsis thaliana
Genetic Insert: Cry2FL-VP64
Vector Backbone Description: Backbone Size:5333; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25664691
Proper citation: RRID:Addgene_60554 Copy
Species: Arabidopsis thaliana
Genetic Insert: dCas9-CibN
Vector Backbone Description: Backbone Size:5333; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25664691
Proper citation: RRID:Addgene_60553 Copy
Species: Synthetic
Genetic Insert: mTurquoise
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4000; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20081836
Proper citation: RRID:Addgene_60559 Copy
Species: Synthetic
Genetic Insert: mTurquoise
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4000; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20081836
Proper citation: RRID:Addgene_60558 Copy
Vector Backbone Description: Backbone Size:7105; Vector Backbone:pDEST-0GWA; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:23471584
Proper citation: RRID:Addgene_60556 Copy
Species: Arabidopsis thaliana
Genetic Insert: CibN-dCas9
Vector Backbone Description: Backbone Size:5333; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25664691
Proper citation: RRID:Addgene_60551 Copy
Species: Synthetic
Genetic Insert: Reporter_9
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25344638
Comments: This reporter plasmid contains the attB and attP sites flanking a green fluorescent protein reporter gene (gfp). A strong constitutive promoter (BBa_J23119) is placed upstream of the attB site, which transcribes in the opposite orientation as gfp. After the integrase is expressed, the orientation of gfp is inverted and it is transcribed. After inversion, recombined attB and attP sites result in the formation of attL and attR. The attL site is located on the 5'UTR and could impact gfp expression. To insulate against this effect, we included a spacer and the ribozyme RiboJ.
Also contains mRFP in order to aid the gating of cells.
Proper citation: RRID:Addgene_60569 Copy
Species: Synthetic
Genetic Insert: Reporter_8
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25344638
Comments: This reporter plasmid contains the attB and attP sites flanking a green fluorescent protein reporter gene (gfp). A strong constitutive promoter (BBa_J23119) is placed upstream of the attB site, which transcribes in the opposite orientation as gfp. After the integrase is expressed, the orientation of gfp is inverted and it is transcribed. After inversion, recombined attB and attP sites result in the formation of attL and attR. The attL site is located on the 5'UTR and could impact gfp expression. To insulate against this effect, we included a spacer and the ribozyme RiboJ.
Also contains mRFP in order to aid the gating of cells.
Proper citation: RRID:Addgene_60568 Copy
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