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Species: Homo sapiens
Genetic Insert: SFRS17A
Vector Backbone Description: Backbone Size:7357; Vector Backbone:E2.pEF5-DEST-FL-V5-MCP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32807991
Proper citation: RRID:Addgene_156010 Copy
Species: Drosophila melanogaster
Genetic Insert: Nibbler
Vector Backbone Description: Vector Backbone:pAFMW; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33199607
Proper citation: RRID:Addgene_188584 Copy
Species: Drosophila melanogaster
Genetic Insert: Nibbler N-terminal domain
Vector Backbone Description: Vector Backbone:pAFMW; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33199607
Proper citation: RRID:Addgene_188586 Copy
Species: Drosophila melanogaster
Genetic Insert: Nibbler C-terminal domain
Vector Backbone Description: Vector Backbone:pAFMW; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33199607
Proper citation: RRID:Addgene_188587 Copy
Species: Listeria monocytogenes
Genetic Insert: ActA tail anchor
Vector Backbone Description: Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22464442
Comments: Transfection of this plasmid will express mCherry-fused to the N-terminus of ActA tail anchor sequence: "LILAMLAIGVFSLGAFIKIIQLRKNN", which targets the mitochondrial outer membrane and signal correlates with mitotracker Green signal in cells. "7aa" indicates the 7 aa flexible linker region, "SGLRSRV" between mCherry and the ActA targeting signal.
Proper citation: RRID:Addgene_177406 Copy
Species: Homo sapiens
Genetic Insert: Bcl-XL
Vector Backbone Description: Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30860026
Comments: Transfection of this plasmid will express mCerulean3-fused to the N-terminus of Bcl-XL. There is a flexible, 7 aa linker "SGLRSRE", between mCerulean3 and Bcl-XL.
Proper citation: RRID:Addgene_177408 Copy
Species: Homo sapiens
Genetic Insert: Bcl-XL
Vector Backbone Description: Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22464442
Comments: Transfection of this plasmid will express Venus-fused to the N-terminus of Bcl-XL. There is a flexible, 7 aa linker "SGLRSTR", between Venus and BclXL. This Venus protein contains an F224R mutation to make it monomeric.
Proper citation: RRID:Addgene_177409 Copy
Species: Homo sapiens
Genetic Insert: PRPF4
Vector Backbone Description: Backbone Size:7357; Vector Backbone:E2.pEF5-DEST-FL-V5-MCP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32807991
Proper citation: RRID:Addgene_155850 Copy
Species: Homo sapiens
Genetic Insert: PRPF40A
Vector Backbone Description: Backbone Size:7357; Vector Backbone:E2.pEF5-DEST-FL-V5-MCP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32807991
Proper citation: RRID:Addgene_155851 Copy
Species: Homo sapiens
Genetic Insert: SMG6
Vector Backbone Description: Backbone Size:7357; Vector Backbone:E2.pEF5-DEST-FL-V5-MCP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32807991
Proper citation: RRID:Addgene_156020 Copy
Species: Homo sapiens
Genetic Insert: SMN1
Vector Backbone Description: Backbone Size:7357; Vector Backbone:E2.pEF5-DEST-FL-V5-MCP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32807991
Proper citation: RRID:Addgene_156022 Copy
Species: Synthetic
Genetic Insert: Membrane-targeted miRFP670
Vector Backbone Description: Backbone Size:6900; Vector Backbone:pPBpuro; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36174555
Proper citation: RRID:Addgene_176030 Copy
Species: Drosophila melanogaster
Genetic Insert: Nibbler
Vector Backbone Description: Vector Backbone:pAFMW; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33199607
Proper citation: RRID:Addgene_188595 Copy
Species: Synthetic
Genetic Insert: Clone 2 light chain (HK02)
Vector Backbone Description: Vector Backbone:pFuse; Vector Types:Mammalian Expression; Bacterial Resistance:Blasticidin
Defining Citation: PMID:35347138
Proper citation: RRID:Addgene_192179 Copy
Species: Drosophila melanogaster
Genetic Insert: GW-peptide (drosophila) N-terminally tagged C-terminal domain of Nibbler
Vector Backbone Description: Vector Backbone:pAFMW; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33199607
Proper citation: RRID:Addgene_188592 Copy
Species: Homo sapiens
Genetic Insert: DDX11
Vector Backbone Description: Backbone Size:7357; Vector Backbone:E2.pEF5-DEST-FL-V5-MCP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32807991
Proper citation: RRID:Addgene_155502 Copy
Species: Synthetic
Genetic Insert: MBD1.MBD-WT-13XL-BASU
Vector Backbone Description: Vector Backbone:parBit_v9; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32123383
Proper citation: RRID:Addgene_179416 Copy
Species: Synthetic
Genetic Insert: MBD1.MBD-R22A-13XL-BASU
Vector Backbone Description: Vector Backbone:parBit_v9; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32123383
Comments: Sequence map can also be found at https://benchling.com/s/seq-O5mtTLe9VzlMwjTIPW4J
Proper citation: RRID:Addgene_179417 Copy
Species: Synthetic
Genetic Insert: Raichu-Ras-HRasCT
Vector Backbone Description: Backbone Size:6800; Vector Backbone:pPB; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36174555
Proper citation: RRID:Addgene_176029 Copy
Species: Caenorhabditis elegans
Genetic Insert: acr-2(3.7k)
Vector Backbone Description: Vector Backbone:pPD95.62; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33378642
Proper citation: RRID:Addgene_191058 Copy
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