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Species: Homo sapiens
Genetic Insert: IL1R2
Vector Backbone Description: Backbone Size:7153; Vector Backbone:pD649; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32822567
Comments: Some plasmids in this collection may have an IS4-like element ISVsa5 family transposase upstream of the CMV promoter.
Proper citation: RRID:Addgene_157333 Copy
Vector Backbone Description: Vector Backbone:pGreen0029; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36352791
Comments: P35S-NC frame-Tnos
Proper citation: RRID:Addgene_193395 Copy
Species: Synthetic
Genetic Insert: human codon-optimized Cas12Max
Vector Backbone Description: Backbone Marker:Feng Zhang (Addgene plasmid # 42230); Vector Backbone:px330; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37378659
Proper citation: RRID:Addgene_195334 Copy
Species: Homo sapiens
Genetic Insert: CEACAM8
Vector Backbone Description: Backbone Size:7153; Vector Backbone:pD649; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32822567
Comments: Some plasmids in this collection may have an IS4-like element ISVsa5 family transposase upstream of the CMV promoter.
Proper citation: RRID:Addgene_157294 Copy
Species: R. flavefaciens
Genetic Insert: high fidelity (hf) version of CasRx (hfCas13d)
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41020266
Proper citation: RRID:Addgene_195864 Copy
Species: R. flavefaciens
Genetic Insert: high fidelity (hf) version of CasRx (hfCas13d)
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41020266
Proper citation: RRID:Addgene_195865 Copy
Species: Synthetic
Genetic Insert: mScarlet-i
Vector Backbone Description: Vector Backbone:p15A; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34985281
Proper citation: RRID:Addgene_190614 Copy
Species: Homo sapiens
Genetic Insert: NGFR
Vector Backbone Description: Backbone Size:7153; Vector Backbone:pD649; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32822567
Comments: Some plasmids in this collection may have an IS4-like element ISVsa5 family transposase upstream of the CMV promoter.
Proper citation: RRID:Addgene_157228 Copy
Species: Other
Genetic Insert: eBFP2-NLS
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28764932
Proper citation: RRID:Addgene_183800 Copy
Species: Synthetic
Genetic Insert: mScarlet-I
Vector Backbone Description: Vector Backbone:Unspecified; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.03.26.437257v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_185943 Copy
Species: Synthetic
Genetic Insert: Ig kappa-FRISZ-PDGFR TM
Vector Backbone Description: Backbone Size:4280; Vector Backbone:pAAV-hSyn; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36857451
Comments: The N-terminal Ig kappa leader sequence is for targeting the synthesized peptide to the secretory pathway. The C-terminal PDGFR transmembrane domain is for anchoring the protein to the mammalian cell surface. AAV derived from this plasmid has been successfully used for wide-field in vivo imaging. We deposit another plasmid, pAAV-hSyn-FRISZ-GPI (Addgene # 177264, not yet tested in vivo), which uses a CD59 leader sequence for secretory pathway targeting and a CD59 GPI for anchoring. We plan to systematically compare various exporting and anchoring strategies.
Please visit https://www.biorxiv.org/content/10.1101/2022.06.02.494512v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_176886 Copy
Species: Synthetic
Genetic Insert: ecTadA(8e)-SpCas9-NG
Vector Backbone Description: Vector Backbone:unknown; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37083425
Comments: Please visit https://www.biorxiv.org/content/10.1101/2023.02.06.527367v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_195343 Copy
Species: Mus musculus
Genetic Insert: C16 Ex3 XTR f1
Vector Backbone Description: Vector Backbone:pYTK084; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37366288
Comments: Please visit https://doi.org/10.1101/2022.11.21.517425 for bioRxiv preprint.
Proper citation: RRID:Addgene_194013 Copy
Species: Homo sapiens
Genetic Insert: Human Reticulon 2 mutant R60f
Vector Backbone Description: Vector Backbone:pLV-eGFP_Addgene#36083; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35609616
Proper citation: RRID:Addgene_186607 Copy
Species: Homo sapiens
Genetic Insert: Human Reticulon 2 isoform B amino acids 272 to 472
Vector Backbone Description: Vector Backbone:mNeonGreen-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35609616
Proper citation: RRID:Addgene_186606 Copy
Species: Homo sapiens
Genetic Insert: Human Reticulon 2 isoform B amino acids 272 to 472
Vector Backbone Description: Vector Backbone:mNeonGreen-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35609616
Proper citation: RRID:Addgene_186605 Copy
Species: Homo sapiens
Genetic Insert: Human Reticulon 2 isoform B amino acids 136 to 271
Vector Backbone Description: Vector Backbone:mNeonGreen-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35609616
Proper citation: RRID:Addgene_186604 Copy
Species: Homo sapiens
Genetic Insert: BTNL9
Vector Backbone Description: Backbone Size:6106; Vector Backbone:pD649; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32822567
Comments: Some plasmids in this collection may have an IS4-like element ISVsa5 family transposase upstream of the CMV promoter.
Proper citation: RRID:Addgene_156665 Copy
Species: Synthetic
Genetic Insert: mEos3.2
Vector Backbone Description: Backbone Marker:Thermo; Vector Backbone:pBAD/HisB; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35903559
Proper citation: RRID:Addgene_189719 Copy
Species: Homo sapiens
Genetic Insert: ACE2
Vector Backbone Description: Vector Backbone:pExp-CHis; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37433017
Comments: LIC cloning used 5' site BsaI (destroyed in cloning) and 3' site HindIII (not destroyed).
Proper citation: RRID:Addgene_194998 Copy
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