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Species: Mus musculus
Genetic Insert: mTiam1-tgRFPt-SSPB R73Q
Vector Backbone Description: Vector Backbone:pLentiLox7.0; Vector Types:Mammalian Expression, Lentiviral, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25535392
Comments: The SspB used in this construct contains two amino acid mutations that disrupt the natural dimerization of SspB. These mutations are Y11K and A15E (residue numbering from pdb code 1ou9). Here is the sequence around the mutated residues with the sites of mutation in parentheses: SSPKRP(K)LLR(E)YYDWLVDNS
Proper citation: RRID:Addgene_60418 Copy
Genetic Insert: See comments
Vector Backbone Description: Vector Backbone:N/A; Vector Types:Synthetic Biology; Bacterial Resistance:None
Defining Citation: PMID:21189298
Comments: MG1655 + lacIq intergrated at intS + Δ dsrA
Proper citation: RRID:Addgene_60371 Copy
Genetic Insert: See comments
Vector Backbone Description: Vector Backbone:N/A; Vector Types:Synthetic Biology; Bacterial Resistance:None
Defining Citation: PMID:21189298
Comments: MG1655 + lacIq intergrated at intS + Δ micC
Proper citation: RRID:Addgene_60370 Copy
Species: Hamster
Genetic Insert: HMG-CoA synthase promoter (-324/-225) fused to HMG-CoA synthase TATAA (-28/+39) transcription initiator sequence
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5357; Vector Backbone:pGL2 basic; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:2903862
Proper citation: RRID:Addgene_60490 Copy
Vector Backbone Description: Backbone Size:2021; Vector Backbone:pUC19; Vector Types:Mammalian Expression, Transposon; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25650551
Proper citation: RRID:Addgene_60496 Copy
Genetic Insert: See comments
Vector Backbone Description: Vector Backbone:N/A; Vector Types:Synthetic Biology; Bacterial Resistance:None
Defining Citation: PMID:21189298
Comments: MG1655 + lacIq intergrated at intS + Δ ryhB
Proper citation: RRID:Addgene_60374 Copy
Vector Backbone Description: Backbone Size:2021; Vector Backbone:pUC19; Vector Types:Mammalian Expression, Transposon; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25650551
Proper citation: RRID:Addgene_60495 Copy
Genetic Insert: See comments
Vector Backbone Description: Vector Backbone:N/A; Vector Types:Synthetic Biology; Bacterial Resistance:None
Defining Citation: PMID:21189298
Comments: MG1655 + lacIq intergrated at intS + Δ hfq + Δ micC
Proper citation: RRID:Addgene_60372 Copy
Species: Synthetic
Genetic Insert: SYFP2-mTurquoise2
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22434194
Proper citation: RRID:Addgene_60493 Copy
Species: H. sodomense
Genetic Insert: Archer1-EGFP
Vector Backbone Description: Backbone Size:9241; Vector Backbone:pLenti; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25222271
Proper citation: RRID:Addgene_60423 Copy
Species: Synthetic
Genetic Insert: CaMPARI
Vector Backbone Description: Backbone Marker:Life Technologies; Backbone Size:5376; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25678659
Proper citation: RRID:Addgene_60421 Copy
Species: Homo sapiens
Genetic Insert: hITSN1-tgRFPt-SSPB
Vector Backbone Description: Vector Backbone:pLentiLox7.0; Vector Types:Mammalian Expression, Lentiviral, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25535392
Comments: The SspB used in this construct contains two amino acid mutations that disrupt the natural dimerization of SspB. These mutations are Y11K and A15E (residue numbering from pdb code 1ou9). Here is the sequence around the mutated residues with the sites of mutation in parentheses: SSPKRP(K)LLR(E)YYDWLVDNS
Proper citation: RRID:Addgene_60420 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: TUB2
Vector Backbone Description: Vector Backbone:pRS424; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24633327
Comments: Johnson V, Ayaz P, Huddleston P, Rice LM.
Design, overexpression, and purification of polymerization-blocked yeast αβ-tubulin mutants.
Biochemistry. 2011 Oct 11;50(40):8636-44. doi: 10.1021/bi2005174. Epub 2011 Sep 16.
Proper citation: RRID:Addgene_60386 Copy
Species: Homo sapiens
Genetic Insert: KLF2
Vector Backbone Description: Backbone Marker:Dr. Hitoshi Niwa; Vector Backbone:hCMV1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25215486
Proper citation: RRID:Addgene_60433 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: TUB2
Vector Backbone Description: Vector Backbone:pRS424; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24633327
Proper citation: RRID:Addgene_60399 Copy
Species: Homo sapiens
Genetic Insert: NANOG
Vector Backbone Description: Backbone Marker:Dr. Hitoshi Niwa; Vector Backbone:hCMV1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25215486
Proper citation: RRID:Addgene_60432 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: TUB2
Vector Backbone Description: Vector Backbone:pRS424; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24633327
Proper citation: RRID:Addgene_60398 Copy
Genetic Insert: rtTA3
Vector Backbone Description: Vector Backbone:N/A; Vector Types:ZFN donor; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25630922
Proper citation: RRID:Addgene_60431 Copy
Vector Backbone Description: Vector Backbone:pPB-PGK; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:25215486
Proper citation: RRID:Addgene_60436 Copy
Species: Homo sapiens
Genetic Insert: KLF4
Vector Backbone Description: Vector Backbone:PGK; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25215486
Proper citation: RRID:Addgene_60435 Copy
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