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  • Organism:arabidopsis thaliana (facet)


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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
pET28-At_PRORP1-His
 
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RRID:Addgene_67869 PRORP1 Arabidopsis thaliana Kanamycin PMID:22976545 The mitochondrial targeting sequence “MLRLTCFTPSFSRACCPLFAMMLKVPSVHLHHPRF“ of native precursor PRORP1 was replaced with the dipeptide “MG” Backbone Marker:Novagen; Backbone Size:5369; Vector Backbone:pET28-b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin Residues 36-572 (see comments) 2026-08-15 01:18:39 0
pMAL-PIAL2M-wt
 
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RRID:Addgene_67909 PIAL2 Arabidopsis thaliana Ampicillin PMID:25415977 Backbone Marker:NEB; Backbone Size:6700; Vector Backbone:pMAL-c2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin E281-A496 2026-08-15 01:18:43 0
pABD823
 
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RRID:Addgene_67911 Cold temperature germinating 10 (CTG10) Arabidopsis thaliana Chloramphenicol PMID:29632208 Backbone Marker:Stratagene; Backbone Size:6494; Vector Backbone:pBD-GAL4 Cam; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol 2026-08-15 01:18:39 0
pET28-At_PRORP2-His
 
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RRID:Addgene_67870 PRORP2 Arabidopsis thaliana Kanamycin PMID:22976545 Backbone Marker:Novagen; Backbone Size:5369; Vector Backbone:pET28-b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:18:39 0
pICSL11062
 
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RRID:Addgene_68256 Promoter_AtU6-26 + sgRNA_BolC.GA4a-2 Arabidopsis thaliana Ampicillin PMID:26616834 Target sequence: GTTGAGAGGGGAGCCGGTGA Vector Backbone:pICH47761 (AddGene #48003); Vector Types:Plant Expression, Synthetic Biology; Bacterial Resistance:Ampicillin 2026-08-15 01:18:41 0
pICSL90002
 
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1+ mentions
RRID:Addgene_68261 Promoter: U6-26 (Arabidopsis thaliana) Arabidopsis thaliana Spectinomycin PMID:26616834 Vector Backbone:pAGM9121; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin 2026-08-15 01:18:42 6
pPAt2S3 (GB0029)
 
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RRID:Addgene_68162 At2S3 promoter Arabidopsis thaliana Ampicillin PMID:23669743 insert can be released with BsaI Backbone Marker:self-made; derived from pGEMT-Easy manufactured by Promega; Vector Backbone:pUPD; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin BsaI and BsmBI sites removed 2026-08-15 01:18:41 0
pPAtUbq10 (GB0223)
 
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1+ mentions
RRID:Addgene_68174 AtUbq10 promoter Arabidopsis thaliana Ampicillin PMID:23669743 insert can be released with BsaI Backbone Marker:self-made; derived from pGEMT-Easy manufactured by Promega; Vector Backbone:pUPD; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin BsaI and BsmBI sites removed 2026-08-15 01:18:41 1
pHsp70B (GB0155)
 
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RRID:Addgene_68172 AtHsp70B promoter Arabidopsis thaliana Ampicillin PMID:23669743 insert can be released with BsaI Backbone Marker:self-made; derived from pGEMT-Easy manufactured by Promega; Vector Backbone:pUPD; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin BsaI and BsmBI sites removed 2026-08-15 01:18:45 0
pHsp18.2 (GB0153)
 
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RRID:Addgene_68171 AtHsp18.2 promoter Arabidopsis thaliana Ampicillin PMID:23669743 insert can be released with BsaI Backbone Marker:self-made; derived from pGEMT-Easy manufactured by Promega; Vector Backbone:pUPD; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin BsaI and BsmBI sites removed 2026-08-15 01:18:41 0
pOPTXcGMPRELUC
 
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1+ mentions
RRID:Addgene_68503 OPTX promoter with cGMPRE Arabidopsis thaliana Kanamycin PMID:24206622 Backbone Marker:Calderon-Villalobos et al 2006 Plant Physiol 141:3-14 (GenBank: AY968054.1); Backbone Size:7272; Vector Backbone:pLUC Trap3 (GW); Vector Types:Bacterial Expression, Plant Expression; Bacterial Resistance:Kanamycin 3x cGMPRE inserted 190bp 5' of ATG (cGMPRE = aaatagatttcaacagtt) 2026-08-15 01:18:44 1
pOPTXGARE
 
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RRID:Addgene_68542 OPTX promoter with GARE Arabidopsis thaliana Kanamycin PMID:24206622 linker sequence between inserted GARE is 5' gagagcc 3' Backbone Marker:Calderon-Villalobos et al 2006 Plant Physiol 141:3-14 (GenBank: AY968054.1); Backbone Size:7272; Vector Backbone:pLUC Trap3 (GW); Vector Types:Bacterial Expression, Plant Expression; Bacterial Resistance:Kanamycin 5x GARE inserted 190 bp 5' pf ATG (GARE = taacaaa) 2026-08-15 01:18:44 0
pDBTrp-LexABD-CRY2FL
 
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RRID:Addgene_78210 LexABD-CRY2 Arabidopsis thaliana Kanamycin PMID:25350266 Backbone Marker:custom ; Vector Backbone:pDBTrp; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin contains endogenous CRY2 NLS 2026-08-15 01:20:22 0
pSpal2At
 
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1+ mentions
RRID:Addgene_78286 PAL2 Arabidopsis thaliana Chloramphenicol PMID:21722749 *Note: Addgene's quality control sequencing finds an E50A amino acid residue substitution in the A. thaliana PAL2 gene insert. The depositing laboratory does not believe this residue change will affect protein function. This plasmid accurately reflects the construct as it was used in the associated publication. Backbone Size:2999; Vector Backbone:pSTV28; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol *see below 2026-08-15 01:20:23 2
INCTbiosyn-p35S-GFP(rc)4
 
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1+ mentions
RRID:Addgene_127522 EGFP reverse complement coding sequence flanked by attB/attP Integrase 4 attachment sites. Arabidopsis thaliana Ampicillin PMID:32444777 The whole insert (promoter, CDS and terminator) was synthesized and cloned into backbone plasmid. The CaMV 35S promoter sequence is derived from iGEM registry (BBa_K1547006); the EGFP coding sequence from Chiu, W. et al. Curr. Biol. 6, 325-330 (1996); the attB/P integrase 4 attachment sites sequences from Yang, L. et al. Nat. Methods 11, 1261-1266 (2014); and the NOS terminator sequence from pBI426 plasmid of Datla, R.S., et al. Gene 101, 239-246 (1991). Vector Backbone:pUC18; Vector Types:Plant Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:04:37 1
INCTbiosyn-p35S-GFP(rc)2
 
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1+ mentions
RRID:Addgene_127521 EGFP reverse complement coding sequence flanked by attB/attP Integrase 2 attachment sites. Arabidopsis thaliana Ampicillin PMID:32444777 The whole insert (promoter, CDS and terminator) was synthesized and cloned into backbone plasmid. The CaMV 35S promoter sequence is derived from iGEM registry (BBa_K1547006); the EGFP coding sequence from Chiu, W. et al. Curr. Biol. 6, 325-330 (1996); the attB/P integrase 2 attachment sites sequences from Yang, L. et al. Nat. Methods 11, 1261-1266 (2014); and the NOS terminator sequence from pBI426 plasmid of Datla, R.S., et al. Gene 101, 239-246 (1991). Vector Backbone:pUC18; Vector Types:Plant Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:04:37 1
INCTbiosyn-p35S(rc)2_4_5-GFP
 
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1+ mentions
RRID:Addgene_127520 CaMV 35S reverse complement promoter sequence flanked by attB/attP Integrase 2, 4 and 5 attachment sites. Arabidopsis thaliana Ampicillin PMID:32444777 The CaMV 35S promoter in a reverse complement sequence orientation came from iGEM registry (BBa_K1547006 ) and the attB/P integrases 2, 4 and 5 attatchment sites flanking this sequence came from Yang, L. et al. Nat. Methods 11, 1261-1266 (2014). This cassete was previously synthesized in pBluescript II SK(-) and after cloned using HindIII and BamHI replacing the forward CaMV 35S promoter sequence from a 35S-GFP plasmid construction of Chiu, W. et al. Curr. Biol. 6, 325-330 (1996). Vector Backbone:pUC18; Vector Types:Plant Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:04:36 1
INCTbiosyn-p35S-GFP(rc)7
 
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1+ mentions
RRID:Addgene_127524 EGFP reverse complement coding sequence flanked by attB/attP Integrase 7 attachment sites. Arabidopsis thaliana Ampicillin PMID:32444777 The whole insert (promoter, CDS and terminator) was synthesized and cloned into backbone plasmid. The CaMV 35S promoter sequence is derived from iGEM registry (BBa_K1547006); the EGFP coding sequence from Chiu, W. et al. Curr. Biol. 6, 325-330 (1996); the attB/P integrase 7 attachment sites sequences from Yang, L. et al. Nat. Methods 11, 1261-1266 (2014); and the NOS terminator sequence from pBI426 plasmid of Datla, R.S., et al. Gene 101, 239-246 (1991). Vector Backbone:pUC18; Vector Types:Plant Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:04:37 1
INCTbiosyn-p35S-GFP(rc)Bxb1
 
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1+ mentions
RRID:Addgene_127528 EGFP reverse complement coding sequence flanked by attB/attP Integrase Bxb1 attachment sites. Arabidopsis thaliana Ampicillin PMID:32444777 The whole insert (promoter, CDS and terminator) was synthesized and cloned into backbone plasmid. The CaMV 35S promoter sequence is derived from iGEM registry (BBa_K1547006); the EGFP coding sequence from Chiu, W. et al. Curr. Biol. 6, 325-330 (1996); the attB/P integrase Bxb1 attachment sites sequences from Bonnet, J. et al. Science 340, 599-603 (2013) (Genbank KC529327.1); and the NOS terminator sequence from pBI426 plasmid of Datla, R.S., et al. Gene 101, 239-246 (1991). Vector Backbone:pBluescript SK(-); Vector Types:Plant Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:04:37 1
pEf1a OsTIR ires NEO pA T2BH
 
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RRID:Addgene_127910 OsTIR Arabidopsis thaliana Ampicillin PMID:34106828 Vector Backbone:pBSK; Vector Types:Transposon; Bacterial Resistance:Ampicillin 2026-08-15 01:04:41 1

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