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Species: Homo sapiens
Genetic Insert: RPS10
Vector Backbone Description: Vector Backbone:pLenti_C_Myc_DDK_IRES_Puro; Vector Types:Lentiviral; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31981475
Proper citation: RRID:Addgene_127138 Copy
Species: Homo sapiens
Genetic Insert: RPS10
Vector Backbone Description: Vector Backbone:pLenti_C_Myc_DDK_IRES_Puro; Vector Types:Lentiviral; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31981475
Comments: Depositor confirms mutation does not affect function.
Proper citation: RRID:Addgene_127129 Copy
Genetic Insert: yhaV toxin
Vector Backbone Description: Vector Backbone:pDS132; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31201277
Proper citation: RRID:Addgene_127449 Copy
Genetic Insert: tse2 toxin
Vector Backbone Description: Vector Backbone:pDS132; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31201277
Proper citation: RRID:Addgene_127448 Copy
Genetic Insert: mqsR toxin
Vector Backbone Description: Vector Backbone:pDS132; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31201277
Proper citation: RRID:Addgene_127447 Copy
Genetic Insert: yhaV toxin
Vector Backbone Description: Vector Backbone:pDS132; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31201277
Proper citation: RRID:Addgene_127446 Copy
Species: Methanosarcina mazei
Genetic Insert: Pyrrolysyl-tRNA synthetase/pyrrolysyl -tRNA pair
Vector Backbone Description: Vector Backbone:pEVOL; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:22289053
Proper citation: RRID:Addgene_127411 Copy
Species: Methanosarcina mazei
Genetic Insert: Pyrrolysyl-tRNA synthetase/pyrrolysyl -tRNA pair
Vector Backbone Description: Vector Backbone:pEVOL; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31398275
Proper citation: RRID:Addgene_127415 Copy
Species: streptococcus pneumoniae
Genetic Insert: DpnII
Vector Backbone Description: Vector Backbone:pBAD33.1; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Proper citation: RRID:Addgene_127413 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: LGF2-Gal11p
Vector Backbone Description: Backbone Marker:Agilent #240067; Vector Backbone:pBT; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31182485
Proper citation: RRID:Addgene_127481 Copy
Genetic Insert: tse2 toxin
Vector Backbone Description: Vector Backbone:pDS132; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31201277
Proper citation: RRID:Addgene_127451 Copy
Genetic Insert: mqsR toxin
Vector Backbone Description: Vector Backbone:pDS132; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31201277
Proper citation: RRID:Addgene_127450 Copy
Genetic Insert: mqsR toxin
Vector Backbone Description: Vector Backbone:pDS132; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31201277
Proper citation: RRID:Addgene_127453 Copy
Species: Synthetic
Genetic Insert: mTurquoise2
Vector Backbone Description: Backbone Size:2200; Vector Backbone:pSB1C3; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:29564885
Comments: The insert mTq2-Tat was designed by G.Grossi and M. Schwarz-Schilling and cloned by A. Dupin and M. Schwarz-Schilling. mTq2 sequence is from Goedhart, J. et al. Nat. Commun. 2012, 3, 751. Tat sequence is from Bayer, T. S. et al. RNA 2005, 11, 1848−57. The peptide linker sequence between between YPet and PCP is TRGGGGS.
Proper citation: RRID:Addgene_127635 Copy
Vector Backbone Description: Vector Backbone:pEMY11; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:29753070
Comments: Promoter characterization cassette for promoter expression strength when integrated into ChrXV of S. cerevisiae.
Proper citation: RRID:Addgene_127714 Copy
Species: Synthetic
Genetic Insert: Synthetic CRISPR array containing four identical T. thermophilus spacers
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:3920; Vector Backbone:pACYCDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31278272
Proper citation: RRID:Addgene_127764 Copy
Species: Homo sapiens
Genetic Insert: A3A-W98Y
Vector Backbone Description: Vector Backbone:pBT195; Vector Types:; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31332326
Comments: Along with pBT195, this plasmid can be used to clone pBT361 by SapI or LguI restriction/ligation (Golden Gate). pBT361 is mutagenic in bacteria and care must be taken during cloning, handling and propagation. Benchling map: https://benchling.com/s/seq-viWaBa6R2LjXykdEZnZo
Proper citation: RRID:Addgene_127846 Copy
Genetic Insert: PSP1 target (GGTT PAM)
Vector Backbone Description: Vector Backbone:pACYC; Vector Types:; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31171706
Proper citation: RRID:Addgene_127926 Copy
Species: From Rhodococcus sp, codon optimized for E. coli
Genetic Insert: CocE
Vector Backbone Description: Vector Backbone:pSB4C5 (iGEM registry); Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31462649
Proper citation: RRID:Addgene_128129 Copy
Genetic Insert: Piq - mRFP, parS
Vector Backbone Description: Vector Backbone:pSC101; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31406375
Comments: Cloning method: Golden Gate.
Proper citation: RRID:Addgene_128294 Copy
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