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Species: Mus musculus
Genetic Insert: MYO5A IQ domain
Vector Backbone Description: Backbone Size:5335; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37516972
Proper citation: RRID:Addgene_198201 Copy
Species: Other
Genetic Insert: HCoV-NL63-nsp6
Vector Backbone Description: Backbone Size:2800; Vector Backbone:pENTR223.1*SfiI; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:37267226
Comments: 5'-linker: SfiI-BamHI-ATG-BamHI (GGCCGTCAaggccaggatccatgggatcc), 3'-linker: PacI-TAA-AsiSI-SfiI (cttaatTAAgacgcgatcgcggcctcatGGGCC).
Proper citation: RRID:Addgene_168915 Copy
Species: Other
Genetic Insert: HCoV-NL63-nsp7
Vector Backbone Description: Backbone Size:2800; Vector Backbone:pENTR223.1*SfiI; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:37267226
Comments: 5'-linker: SfiI-BamHI-ATG-BamHI (GGCCGTCAaggccaggatccatgggatcc), 3'-linker: PacI-TAA-AsiSI-SfiI (cttaatTAAgacgcgatcgcggcctcatGGGCC).
Proper citation: RRID:Addgene_168916 Copy
Species: Synthetic
Genetic Insert: mScarlet
Vector Backbone Description: Backbone Size:5306; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36123532
Comments: The mScarlet coding sequence was PCR amplified with an N-terminal 6xHis tag from pCytERM_mScarlet_N1 (Addgene #85066) and inserted into the vector fragment of pET28a (Novagen #69864) digested with NcoI and BamHI.
Proper citation: RRID:Addgene_178543 Copy
Species: Other
Genetic Insert: HCoV-HKU1-nsp12
Vector Backbone Description: Backbone Size:2800; Vector Backbone:pENTR223.1*SfiI; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:37267226
Comments: 5'-linker: SfiI-BamHI-ATG-BamHI (GGCCGTCAaggccaggatccatgggatcc), 3'-linker: PacI-TAA-AsiSI-SfiI (cttaatTAAgacgcgatcgcggcctcatGGGCC).
Proper citation: RRID:Addgene_168909 Copy
Species: Other
Genetic Insert: HCoV-HKU1-Membrane
Vector Backbone Description: Backbone Size:2800; Vector Backbone:pENTR223.1*SfiI; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:37267226
Comments: 5'-linker: SfiI-BamHI-ATG-BamHI (GGCCGTCAaggccaggatccatgggatcc), 3'-linker: PacI-TAA-AsiSI-SfiI (cttaatTAAgacgcgatcgcggcctcatGGGCC).
Proper citation: RRID:Addgene_168905 Copy
Species: Other
Genetic Insert: HCoV-HKU1-nsp9
Vector Backbone Description: Backbone Size:2800; Vector Backbone:pENTR223.1*SfiI; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:37267226
Comments: 5'-linker: SfiI-BamHI-ATG-BamHI (GGCCGTCAaggccaggatccatgggatcc), 3'-linker: PacI-TAA-AsiSI-SfiI (cttaatTAAgacgcgatcgcggcctcatGGGCC).
Proper citation: RRID:Addgene_168898 Copy
Species: Other
Genetic Insert: HCoV-HKU1-nsp10
Vector Backbone Description: Backbone Size:2800; Vector Backbone:pENTR223.1*SfiI; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:37267226
Comments: 5'-linker: SfiI-BamHI-ATG-BamHI (GGCCGTCAaggccaggatccatgggatcc), 3'-linker: PacI-TAA-AsiSI-SfiI (cttaatTAAgacgcgatcgcggcctcatGGGCC).
Proper citation: RRID:Addgene_168899 Copy
Species: Other
Genetic Insert: HCoV-NL63-nsp3
Vector Backbone Description: Backbone Size:2800; Vector Backbone:pENTR223.1*SfiI; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:37267226
Comments: 5'-linker: SfiI-BamHI-ATG-BamHI (GGCCGTCAaggccaggatccatgggatcc), 3'-linker: PacI-TAA-AsiSI-SfiI (cttaatTAAgacgcgatcgcggcctcatGGGCC).
Proper citation: RRID:Addgene_168930 Copy
Species: Other
Genetic Insert: HCoV-OC43-nsp14
Vector Backbone Description: Backbone Size:2800; Vector Backbone:pENTR223.1*SfiI; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:37267226
Comments: 5'-linker: SfiI-BamHI-ATG-BamHI (GGCCGTCAaggccaggatccatgggatcc), 3'-linker: PacI-TAA-AsiSI-SfiI (cttaatTAAgacgcgatcgcggcctcatGGGCC).
Proper citation: RRID:Addgene_168939 Copy
Species: Other
Genetic Insert: HCoV-OC43-nsp7
Vector Backbone Description: Backbone Size:2800; Vector Backbone:pENTR223.1*SfiI; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:37267226
Comments: 5'-linker: SfiI-BamHI-ATG-BamHI (GGCCGTCAaggccaggatccatgggatcc), 3'-linker: PacI-TAA-AsiSI-SfiI (cttaatTAAgacgcgatcgcggcctcatGGGCC).
Proper citation: RRID:Addgene_168935 Copy
Species: Other
Genetic Insert: HCoV-OC43-nsp8
Vector Backbone Description: Backbone Size:2800; Vector Backbone:pENTR223.1*SfiI; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:37267226
Comments: 5'-linker: SfiI-BamHI-ATG-BamHI (GGCCGTCAaggccaggatccatgggatcc), 3'-linker: PacI-TAA-AsiSI-SfiI (cttaatTAAgacgcgatcgcggcctcatGGGCC).
Proper citation: RRID:Addgene_168936 Copy
Species: Other
Genetic Insert: HCoV-NL63-nsp14
Vector Backbone Description: Backbone Size:2800; Vector Backbone:pENTR223.1*SfiI; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:37267226
Comments: 5'-linker: SfiI-BamHI-ATG-BamHI (GGCCGTCAaggccaggatccatgggatcc), 3'-linker: PacI-TAA-AsiSI-SfiI (cttaatTAAgacgcgatcgcggcctcatGGGCC).
Proper citation: RRID:Addgene_168920 Copy
Species: Other
Genetic Insert: HCoV-NL63-nsp15
Vector Backbone Description: Backbone Size:2800; Vector Backbone:pENTR223.1*SfiI; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:37267226
Comments: 5'-linker: SfiI-BamHI-ATG-BamHI (GGCCGTCAaggccaggatccatgggatcc), 3'-linker: PacI-TAA-AsiSI-SfiI (cttaatTAAgacgcgatcgcggcctcatGGGCC).
Proper citation: RRID:Addgene_168921 Copy
Species: Other
Genetic Insert: HCoV-NL63-nsp16
Vector Backbone Description: Backbone Size:2800; Vector Backbone:pENTR223.1*SfiI; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:37267226
Comments: 5'-linker: SfiI-BamHI-ATG-BamHI (GGCCGTCAaggccaggatccatgggatcc), 3'-linker: PacI-TAA-AsiSI-SfiI (cttaatTAAgacgcgatcgcggcctcatGGGCC).
Please note that a transposon was detected in the plasmid backbone. The plasmid is still useful for gateway cloning. Please see the Addgene NGS result for reference.
Proper citation: RRID:Addgene_168922 Copy
Vector Backbone Description: Backbone Size:18469; Vector Backbone:pAGE2.0; Vector Types:Bacterial Expression, Yeast Expression, Synthetic Biology, Diatom Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:37850145
Proper citation: RRID:Addgene_194639 Copy
Species: Other
Genetic Insert: HCoV-NL63-nsp13
Vector Backbone Description: Backbone Size:2800; Vector Backbone:pENTR223.1*SfiI; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:37267226
Comments: 5'-linker: SfiI-BamHI-ATG-BamHI (GGCCGTCAaggccaggatccatgggatcc), 3'-linker: PacI-TAA-AsiSI-SfiI (cttaatTAAgacgcgatcgcggcctcatGGGCC).
Proper citation: RRID:Addgene_168928 Copy
Species: Other
Genetic Insert: HCoV-NL63-Spike
Vector Backbone Description: Backbone Size:2800; Vector Backbone:pENTR223.1*SfiI; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:37267226
Comments: 5'-linker: SfiI-BamHI-ATG-BamHI (GGCCGTCAaggccaggatccatgggatcc), 3'-linker: PacI-TAA-AsiSI-SfiI (cttaatTAAgacgcgatcgcggcctcatGGGCC).
Proper citation: RRID:Addgene_168929 Copy
Species: Other
Genetic Insert: HCoV-NL63-Membrane
Vector Backbone Description: Backbone Size:2800; Vector Backbone:pENTR223.1*SfiI; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:37267226
Comments: 5'-linker: SfiI-BamHI-ATG-BamHI (GGCCGTCAaggccaggatccatgggatcc), 3'-linker: PacI-TAA-AsiSI-SfiI (cttaatTAAgacgcgatcgcggcctcatGGGCC).
Proper citation: RRID:Addgene_168925 Copy
Species: Other
Genetic Insert: HCoV-NL63-Nucleocapsid
Vector Backbone Description: Backbone Size:2800; Vector Backbone:pENTR223.1*SfiI; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:37267226
Comments: 5'-linker: SfiI-BamHI-ATG-BamHI (GGCCGTCAaggccaggatccatgggatcc), 3'-linker: PacI-TAA-AsiSI-SfiI (cttaatTAAgacgcgatcgcggcctcatGGGCC).
Proper citation: RRID:Addgene_168926 Copy
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