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Genetic Insert: Sh-CAST sgRNA 2300
Vector Backbone Description: Backbone Size:5218; Vector Backbone:pUC; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin and Tetracycline
Defining Citation: PMID:37368499
Proper citation: RRID:Addgene_170985 Copy
Genetic Insert: Sh-CAST sgRNA 2300
Vector Backbone Description: Backbone Size:5158; Vector Backbone:pUC; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37368499
Proper citation: RRID:Addgene_170986 Copy
Genetic Insert: mRFP
Vector Backbone Description: Backbone Size:5405; Vector Backbone:pUC; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin and Tetracycline
Defining Citation: PMID:37368499
Proper citation: RRID:Addgene_170983 Copy
Genetic Insert: Sh-CAST sgRNA 2300
Vector Backbone Description: Backbone Size:4812; Vector Backbone:pUC; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:37368499
Proper citation: RRID:Addgene_170984 Copy
Genetic Insert: lacI
Vector Backbone Description: Backbone Size:4787; Vector Backbone:pBBR; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37368499
Proper citation: RRID:Addgene_170981 Copy
Genetic Insert: mRFP
Vector Backbone Description: Backbone Size:4879; Vector Backbone:pUC; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:37368499
Proper citation: RRID:Addgene_170982 Copy
Species: Homo sapiens
Genetic Insert: eSrtA-LPETG-MT4
Vector Backbone Description: Vector Backbone:pETM11; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37434134
Comments: The human MT4 sequence with two Cys residues at positions 30 and 31 is a natural variant and will not affect plasmid function.
Proper citation: RRID:Addgene_200306 Copy
Genetic Insert: cre
Vector Backbone Description: Backbone Marker:addgene Plasmid #135177; Vector Backbone:pAAV-TRE-flex-Clover; Vector Types:Mammalian Expression, Bacterial Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37196659
Comments: Please visit https://doi.org/10.1101/2021.12.26.474213 for bioRxiv preprint.
Proper citation: RRID:Addgene_201198 Copy
Species: Synthetic
Genetic Insert: (NLS)SV40A5-EGFP-6PrA
Vector Backbone Description: Vector Backbone:pEGFP-C3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35681009
Comments: Internal lab code: IG044/P297
Proper citation: RRID:Addgene_201351 Copy
Species: Homo sapiens
Genetic Insert: ATG13, ULK1
Vector Backbone Description: Vector Backbone:pCAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40442316
Comments: ATG13 (450-517)-5aa linker-ULK1 (836-1050)-TEVsite-TwinStrep-Flag-stop
Proper citation: RRID:Addgene_203549 Copy
Species: Synthetic
Genetic Insert: (NLS)SV40A4-EGFP-4PrA
Vector Backbone Description: Vector Backbone:pEGFP-C3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35681009
Comments: Internal lab code: IG060/P313
Proper citation: RRID:Addgene_201360 Copy
Species: Synthetic
Genetic Insert: (NLS)SV40-EGFP
Vector Backbone Description: Vector Backbone:pEGFP-C3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35681009
Comments: Internal lab code: IG063/P523
Proper citation: RRID:Addgene_201363 Copy
Species: Synthetic
Genetic Insert: tagRFPt
Vector Backbone Description: Vector Backbone:pPOT; Vector Types:Unspecified; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40169015
Comments: Please visit https://doi.org/10.1101/2023.04.21.537815 for bioRxiv preprint.
A R102C mutation was found in puromycin that does not affect plasmid function.
Proper citation: RRID:Addgene_201089 Copy
Species: Synthetic
Genetic Insert: mScaret-I
Vector Backbone Description: Vector Backbone:pPOT; Vector Types:Unspecified; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40169015
Comments: Please visit https://doi.org/10.1101/2023.04.21.537815 for bioRxiv preprint.
A R102C mutation was found in puromycin that does not affect plasmid function.
Proper citation: RRID:Addgene_201093 Copy
Species: Homo sapiens
Genetic Insert: ATG13
Vector Backbone Description: Vector Backbone:pCAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40442316
Comments: GST-TEVsite-ATG13 (6-196)-stop
Proper citation: RRID:Addgene_203559 Copy
Species: Synthetic
Genetic Insert: (NLS)SV40-EGFP-4PrA
Vector Backbone Description: Vector Backbone:pEGFP-C3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35681009
Comments: Internal lab code: IG072/P532
Proper citation: RRID:Addgene_201371 Copy
Species: Homo sapiens
Genetic Insert: ATG13
Vector Backbone Description: Vector Backbone:pCAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40442316
Comments: GST-TEVsite-ATG13 (460-517)-stop
Proper citation: RRID:Addgene_203552 Copy
Species: Homo sapiens
Genetic Insert: ATG14
Vector Backbone Description: Vector Backbone:pCAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40442316
Comments: GST-TEVsite-ATG14-stop
Proper citation: RRID:Addgene_203566 Copy
Species: Homo sapiens
Genetic Insert: HES2
Vector Backbone Description: Backbone Marker:Broad Institute Genetic Perturbation Platform; Backbone Size:8264; Vector Backbone:pLX_TRC317; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36608654
Comments: NM_019089,ENST00000377834 . The transcription factor ORF portion of this plasmid was synthesized by Genewiz. Please test multiple small colonies in case of plasmid recombination. To make this collection available in a timely manner, a portion of this collection was not fully sequenced by Addgene. If an Addgene verified full plasmid sequence is not available, please contact us at [email protected] prior to placing an order to request the full sequence.
Proper citation: RRID:Addgene_142401 Copy
Species: Bacillus subtilis
Genetic Insert: PadR
Vector Backbone Description: Vector Backbone:pCS-lpp0.2-PadR; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33378169
Proper citation: RRID:Addgene_201789 Copy
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