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Genetic Insert: sgRNA
Vector Backbone Description: Backbone Size:2597; Vector Backbone:AAV; Vector Types:Mammalian Expression, Mouse Targeting, AAV, Cre/Lox, CRISPR, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25263330
Comments: Information for Cas9 mice:
JAX Stock#024857 B6.129S-Gt(ROSA)26Sor
Proper citation: RRID:Addgene_60226 Copy
Species: Mus musculus
Genetic Insert: TUT4
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pCI; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25175028
Proper citation: RRID:Addgene_60220 Copy
Species: Homo sapiens
Genetic Insert: Argonaute2
Vector Backbone Description: Backbone Size:13918; Vector Backbone:pSLIK-Hygro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22503104
Proper citation: RRID:Addgene_60234 Copy
Genetic Insert: inmCherry
Vector Backbone Description: Backbone Size:6587; Vector Backbone:pUCHR; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26269177
Proper citation: RRID:Addgene_60239 Copy
Genetic Insert: inmCherry
Vector Backbone Description: Backbone Size:6419; Vector Backbone:pUCHR; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26269177
Proper citation: RRID:Addgene_60238 Copy
Genetic Insert: sgRNA
Vector Backbone Description: Backbone Size:2597; Vector Backbone:AAV; Vector Types:Mammalian Expression, Mouse Targeting, AAV, Cre/Lox, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25263330
Comments: Information for Cas9 mice:
JAX Stock#024857 B6.129S-Gt(ROSA)26Sor
Proper citation: RRID:Addgene_60231 Copy
Genetic Insert: sgRNA
Vector Backbone Description: Backbone Size:2597; Vector Backbone:AAV; Vector Types:Mammalian Expression, Mouse Targeting, AAV, Cre/Lox, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25263330
Comments: Information for Cas9 mice:
JAX Stock#024857 B6.129S-Gt(ROSA)26Sor
Proper citation: RRID:Addgene_60230 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: TUB2
Vector Backbone Description: Vector Backbone:pRS424; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24633327
Proper citation: RRID:Addgene_60401 Copy
Species: Escherichia coli
Genetic Insert: RNase HI-D10R-E48R
Vector Backbone Description: Backbone Size:5044; Vector Backbone:pICE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25030905
Proper citation: RRID:Addgene_60367 Copy
Vector Backbone Description: Backbone Size:4384; Vector Backbone:pBAM1; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:21342504
Proper citation: RRID:Addgene_60487 Copy
Species: Escherichia coli
Genetic Insert: RNase HI
Vector Backbone Description: Backbone Size:5044; Vector Backbone:pICE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25030905
Proper citation: RRID:Addgene_60365 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: TUB2
Vector Backbone Description: Vector Backbone:pRS424; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24633327
Comments: C-terminal tail is from TUBB6 (tubulin, beta 6 class V) according to HUGO nomenclature: http://www.genenames.org/genefamilies/TUB
Proper citation: RRID:Addgene_60403 Copy
Species: A. sativa, E. coli
Genetic Insert: iLID C530M
Vector Backbone Description: Backbone Marker:Qiagen; Backbone Size:4800; Vector Backbone:pQE-80L; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25535392
Proper citation: RRID:Addgene_60408 Copy
Species: Mus musculus
Genetic Insert: GMAP210
Vector Backbone Description: Backbone Marker:Sigma; Vector Backbone:p3XFLAG-myc-CMV-26; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19112494
Proper citation: RRID:Addgene_60407 Copy
Species: Homo sapiens
Genetic Insert: KIF17
Vector Backbone Description: Vector Backbone:pHR; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24633327
Proper citation: RRID:Addgene_60406 Copy
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4733; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25030905
Proper citation: RRID:Addgene_60360 Copy
Species: Synthetic
Genetic Insert: Venus-oLID-CAAX
Vector Backbone Description: Vector Backbone:pLentiLox7.0; Vector Types:Mammalian Expression, Lentiviral, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25535392
Proper citation: RRID:Addgene_60412 Copy
Genetic Insert: See comments
Vector Backbone Description: Vector Backbone:N/A; Vector Types:Synthetic Biology; Bacterial Resistance:None
Defining Citation: PMID:21189298
Comments: MG1655 + lacIq intergrated at intS + Δ hfq + Δ ryhB
Proper citation: RRID:Addgene_60376 Copy
Species: Synthetic
Genetic Insert: tgRFPt-SSPB
Vector Backbone Description: Vector Backbone:pLentiLox7.0; Vector Types:Mammalian Expression, Lentiviral, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25535392
Comments: The SspB used in this construct contains two amino acid mutations that disrupt the natural dimerization of SspB. These mutations are Y11K and A15E (residue numbering from pdb code 1ou9). Here is the sequence around the mutated residues with the sites of mutation in parentheses: SSPKRP(K)LLR(E)YYDWLVDNS
Proper citation: RRID:Addgene_60415 Copy
Species: Homo sapiens
Genetic Insert: Venus-oLID-Mito
Vector Backbone Description: Vector Backbone:pLentiLox7.0; Vector Types:Mammalian Expression, Lentiviral, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25535392
Proper citation: RRID:Addgene_60414 Copy
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