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Species: Other
Genetic Insert: HCoV-OC43-NS12.9
Vector Backbone Description: Backbone Size:2800; Vector Backbone:pENTR223.1*SfiI; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:37267226
Comments: 5'-linker: SfiI-BamHI-ATG-BamHI (GGCCGTCAaggccaggatccatgggatcc), 3'-linker: PacI-TAA-AsiSI-SfiI (cttaatTAAgacgcgatcgcggcctcatGGGCC).
Proper citation: RRID:Addgene_168944 Copy
Species: Escherichia coli and Branchiostoma lanceolatum
Genetic Insert: TatA, TatBmNeon, TatC
Vector Backbone Description: Backbone Size:4596; Vector Backbone:pBAD22; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36123532
Comments: The mNeonGreen coding sequence was PCR amplified from pH6-mNeon (Addgene #178460) with KpnI and SpeI ends and inserted into the vector fragment of pTatABcherryC (Addgene #178545) digested with the same enzymes.
Proper citation: RRID:Addgene_178464 Copy
Species: Branchiostoma lanceolatum
Genetic Insert: mNeonGreen dimer
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5303; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36123532
Comments: The mNeonGreen coding sequence was PCR amplified from pH6-mNeon (Addgene #178460) with an SpeI site at both ends and inserted into the vector fragment of pH6-mNeon digested with SpeI.
Proper citation: RRID:Addgene_178462 Copy
Species: Branchiostoma lanceolatum
Genetic Insert: mNeonGreen dimer
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5303; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36123532
Comments: The mNeonGreen coding sequence was PCR amplified from pH6-mNeon (Addgene #178460) with an SpeI site at both ends and inserted into the vector fragment of pH6-mNeonC (Addgene #178461) digested with SpeI.
Proper citation: RRID:Addgene_178463 Copy
Species: Other
Genetic Insert: HCoV-OC43-nsp2
Vector Backbone Description: Backbone Size:2800; Vector Backbone:pENTR223.1*SfiI; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:37267226
Comments: 5'-linker: SfiI-BamHI-ATG-BamHI (GGCCGTCAaggccaggatccatgggatcc), 3'-linker: PacI-TAA-AsiSI-SfiI (cttaatTAAgacgcgatcgcggcctcatGGGCC).
Proper citation: RRID:Addgene_168948 Copy
Species: Branchiostoma lanceolatum
Genetic Insert: mNeonGreen
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5303; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36123532
Comments: The mNeonGreen coding sequence was PCR amplified from L40C-CRISPR.EFS.mNeon (Addgene #69146) and inserted into the vector fragment of pET28a_mScarlet (Addgene #178543) digested with SpeI and BamHI.
Proper citation: RRID:Addgene_178460 Copy
Genetic Insert: Tyr to Phe mutant FAKSPARK2 sensor
Vector Backbone Description: Vector Backbone:pCS2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37349581
Proper citation: RRID:Addgene_195264 Copy
Species: Other
Genetic Insert: HCoV-229E-nsp13
Vector Backbone Description: Backbone Size:2800; Vector Backbone:pENTR223.1*SfiI; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:37267226
Comments: 5'-linker: SfiI-BamHI-ATG-BamHI (GGCCGTCAaggccaggatccatgggatcc), 3'-linker: PacI-TAA-AsiSI-SfiI (cttaatTAAgacgcgatcgcggcctcatGGGCC).
Proper citation: RRID:Addgene_168970 Copy
Species: Other
Genetic Insert: HCoV-229E-Membrane
Vector Backbone Description: Backbone Size:2800; Vector Backbone:pENTR223.1*SfiI; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:37267226
Comments: 5'-linker: SfiI-BamHI-ATG-BamHI (GGCCGTCAaggccaggatccatgggatcc), 3'-linker: PacI-TAA-AsiSI-SfiI (cttaatTAAgacgcgatcgcggcctcatGGGCC).
Proper citation: RRID:Addgene_168966 Copy
Species: Other
Genetic Insert: HCoV-229E-Nucleocapsid
Vector Backbone Description: Backbone Size:2800; Vector Backbone:pENTR223.1*SfiI; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:37267226
Comments: 5'-linker: SfiI-BamHI-ATG-BamHI (GGCCGTCAaggccaggatccatgggatcc), 3'-linker: PacI-TAA-AsiSI-SfiI (cttaatTAAgacgcgatcgcggcctcatGGGCC).
Proper citation: RRID:Addgene_168967 Copy
Species: Other
Genetic Insert: HCoV-229E-nsp15
Vector Backbone Description: Backbone Size:2800; Vector Backbone:pENTR223.1*SfiI; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:37267226
Comments: 5'-linker: SfiI-BamHI-ATG-BamHI (GGCCGTCAaggccaggatccatgggatcc), 3'-linker: PacI-TAA-AsiSI-SfiI (cttaatTAAgacgcgatcgcggcctcatGGGCC).
Proper citation: RRID:Addgene_168961 Copy
Genetic Insert: ATMSPARK2 sensor
Vector Backbone Description: Vector Backbone:lenti vector; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36857446
Proper citation: RRID:Addgene_195249 Copy
Species: Synthetic
Genetic Insert: mVenus-3AA-mTurquoise2
Vector Backbone Description: Backbone Size:5335; Vector Backbone:pcDNA3.1+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37516972
Proper citation: RRID:Addgene_198197 Copy
Species: Other
Genetic Insert: HCoV-229E-nsp2
Vector Backbone Description: Backbone Size:2800; Vector Backbone:pENTR223.1*SfiI; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:37267226
Comments: 5'-linker: SfiI-BamHI-ATG-BamHI (GGCCGTCAaggccaggatccatgggatcc), 3'-linker: PacI-TAA-AsiSI-SfiI (cttaatTAAgacgcgatcgcggcctcatGGGCC).
Proper citation: RRID:Addgene_168968 Copy
Species: Other
Genetic Insert: HCoV-229E-nsp12
Vector Backbone Description: Backbone Size:2800; Vector Backbone:pENTR223.1*SfiI; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:37267226
Comments: 5'-linker: SfiI-BamHI-ATG-BamHI (GGCCGTCAaggccaggatccatgggatcc), 3'-linker: PacI-TAA-AsiSI-SfiI (cttaatTAAgacgcgatcgcggcctcatGGGCC).
Proper citation: RRID:Addgene_168969 Copy
Species: Escherichia coli
Genetic Insert: Oxygen inducible promoter
Vector Backbone Description: Backbone Size:5591; Vector Backbone:pMUT2; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36449712
Proper citation: RRID:Addgene_192863 Copy
Species: Other
Genetic Insert: HCoV-HKU1-nsp13
Vector Backbone Description: Backbone Size:2800; Vector Backbone:pENTR223.1*SfiI; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:37267226
Comments: 5'-linker: SfiI-BamHI-ATG-BamHI (GGCCGTCAaggccaggatccatgggatcc), 3'-linker: PacI-TAA-AsiSI-SfiI (cttaatTAAgacgcgatcgcggcctcatGGGCC).
Proper citation: RRID:Addgene_168910 Copy
Genetic Insert: Surface marker and Citrine reporter
Vector Backbone Description: Vector Backbone:pAAVS1 donor; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37020022
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.08.26.505496v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_196545 Copy
Vector Backbone Description: Backbone Size:57000; Vector Backbone:pTA-Mob 2.0; Vector Types:Bacterial Expression, Yeast Expression, Synthetic Biology, Diatom Expression; Bacterial Resistance:Gentamicin
Defining Citation: PMID:37850145
Proper citation: RRID:Addgene_194645 Copy
Species: Other
Genetic Insert: HCoV-NL63-nsp10
Vector Backbone Description: Backbone Size:2800; Vector Backbone:pENTR223.1*SfiI; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:37267226
Comments: 5'-linker: SfiI-BamHI-ATG-BamHI (GGCCGTCAaggccaggatccatgggatcc), 3'-linker: PacI-TAA-AsiSI-SfiI (cttaatTAAgacgcgatcgcggcctcatGGGCC).
Proper citation: RRID:Addgene_168919 Copy
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