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Genetic Insert: eGFP
Vector Backbone Description: Backbone Marker:Matthew Bogyo laboratory; Backbone Size:6126; Vector Backbone:Addgene Plasmid #38251 (pET22b-CPDSalI); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19956581
Comments: Encodes untagged eGFP fused to Vibrio cholerae MARTX toxin cysteine protease domain (CPD) to be used as a positive control for protein purification
Proper citation: RRID:Addgene_38257 Copy
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:6002; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19956581
Comments: For protein purification, target protein of interest should be cloned into NcoI and SalI sites to create fusion with Vibrio cholerae MARTX toxin cysteine protease domain (CPD)
Proper citation: RRID:Addgene_38252 Copy
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:6153; Vector Backbone:pET22b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19956581
Comments: For protein purification, target protein of interest should be cloned into NdeI and SalI sites to create HA-tagged fusion with Vibrio cholerae MARTX toxin cysteine protease domain (CPD). HA epitope tag will remain on target protein after cleavage.
Proper citation: RRID:Addgene_38253 Copy
Species: Synthetic
Genetic Insert: E2-Crimson
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5603; Vector Backbone:pEF/myc/ER; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19658435
Comments: Vector for targeting E2-Crimson to the ER in mammalian cells.
Substitutions in E2-Crimson relative to DsRed-Express2 are: E32V, Q66F, V71A, V73I, K83L, L85Q, F118L, L150N, I161N, K163M, V175C, Y193H, S197Y
IMPORTANT NOTE: There is an 82-bp intron after the ER signal sequence. The signal sequence will be in frame with the final product.
Proper citation: RRID:Addgene_38770 Copy
Species: Homo sapiens
Genetic Insert: β₂AR
Vector Backbone Description: Vector Backbone:pcDNA3.1-mCFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16687412
Comments: The Lefkowitz Lab has confirmed this plasmid contains the human gene, and not the rat version described in the publication.
Proper citation: RRID:Addgene_38260 Copy
Species: Homo sapiens
Genetic Insert: Rif
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15668168
Comments: human Rif GTPase (RHOF) with N-terminal myc-tag in pcDNA3. Constitutively active point mutation of Q77L
Proper citation: RRID:Addgene_38768 Copy
Species: Homo sapiens
Genetic Insert: RHOD
Vector Backbone Description: Backbone Marker:SGC; Vector Backbone:pNIC28-Bsa4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: http://www.thesgc.org/structures/2J1L/
Proper citation: RRID:Addgene_38802 Copy
Species: Homo sapiens
Genetic Insert: PICK1
Vector Backbone Description: Backbone Marker:SGC; Vector Backbone:pNIC28-Bsa4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: http://www.thesgc.org/structures/2GZV/
Proper citation: RRID:Addgene_38803 Copy
Species: Homo sapiens
Genetic Insert: RRAD
Vector Backbone Description: Backbone Marker:SGC; Vector Backbone:pNIC28-Bsa4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: http://www.thesgc.org/structures/2GJS/
Proper citation: RRID:Addgene_38804 Copy
Species: Drosophila melanogaster
Genetic Insert: zw4
Vector Backbone Description: Backbone Size:8904; Vector Backbone:pUAST; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9012507
Comments: Perrimon Lab plasmid #77
Proper citation: RRID:Addgene_38764 Copy
Species: Mus musculus
Genetic Insert: Tiar
Vector Backbone Description: Backbone Size:4500; Vector Backbone:pLJM60; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22552098
Proper citation: RRID:Addgene_38244 Copy
Species: Drosophila melanogaster
Genetic Insert: dishevelled
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBlueScript; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8288125
Comments: Perrimon Lab plasmid #17
Proper citation: RRID:Addgene_38342 Copy
Species: Drosophila melanogaster
Genetic Insert: spitz
Vector Backbone Description: Backbone Size:7855; Vector Backbone:pCaSpeR-4; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:1644292
Comments: Perrimon Lab plasmid #10
Proper citation: RRID:Addgene_38340 Copy
Species: Drosophila melanogaster
Genetic Insert: djun
Vector Backbone Description: Backbone Size:8900; Vector Backbone:pCaSpeR-hs; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:1696724
Comments: Perrimon Lab plasmid #7
Proper citation: RRID:Addgene_38337 Copy
Species: Mus musculus
Genetic Insert: Septin 7
Vector Backbone Description: Backbone Marker:Ambion; Backbone Size:3130; Vector Backbone:pSilencer 2.0-U6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19043408
Proper citation: RRID:Addgene_38299 Copy
Species: Escherichia coli
Genetic Insert: E. coli polyphosphate kinase
Vector Backbone Description: Backbone Marker:K. Melcher; Vector Backbone:pKM263; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_38333 Copy
Species: Escherichia coli
Genetic Insert: E. coli polyphosphate kinase
Vector Backbone Description: Backbone Size:5389; Vector Backbone:pETM-41; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_38334 Copy
Species: Mus musculus
Genetic Insert: Septin 6
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22232702
Proper citation: RRID:Addgene_38296 Copy
Species: Mus musculus
Genetic Insert: Myosin II A
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19201857
Proper citation: RRID:Addgene_38297 Copy
Species: Escherichia coli
Genetic Insert: Lon protease fragment
Vector Backbone Description: Vector Backbone:pETM41; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:17320430
Comments: See "Nomura, K., Kato, J., Takiguchi, N., Ohtake, H., Kuroda, A., 2004. Effects of inorganic polyphosphate on the proteolytic and DNA-binding activities of Lon in Escherichia coli. J. Biol. Chem. 279, 34406–34410." for more information.
Proper citation: RRID:Addgene_38330 Copy
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