Searching the RRID Resource Information Network
Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.
| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
Comments |
||||
|---|---|---|---|---|---|---|---|---|---|---|
|
pTH751-CEN-RLuc/min53maxCFLuc Resource Report Resource Website |
RRID:Addgene_38217 | Firefly Luciferase | Photinus pyralis | Ampicillin | PMID:24357599 | Backbone Size:6616; Vector Backbone:pCENBEVY-U; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin | The first 53 codons of the original FLuc gene were exchanged for those isoaccepting codons decoded by the least abundant tRNAs in yeast. All remaining codons were exchanged for those isoaccepting codons decoded by the most abundant tRNAs. The last three codons of the full-length Firefly luciferase gene have been deleted to yield a luciferase variant which is fully functional, but no longer localises to peroxisomes. | 2026-08-15 01:14:20 | 0 | |
|
pCDNA3.1Xpress-AbrGAP Resource Report Resource Website |
RRID:Addgene_38177 | Abr GAP domain | Homo sapiens | Ampicillin | PMID:17116687 | Constructed by Young Jin Cho. An 0.8 kb BamHI xEcoRI fragment from AbrGAP in pGEX3x (AddGene #36407) was inserted into pcDNA BamHI xEcoRI. The insert starts at a natural PvuII site in the ABR sequence. The fragment contains the natural TAG and 3’ UT sequences and encodes amino acid residues 597-859 (Abr isoform B). | Backbone Size:5500; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | ABR GAP domain including amino acids 597-859 | 2026-08-15 01:14:19 | 0 |
|
pTH747-CEN-RLuc/min4maxCFLuc Resource Report Resource Website |
RRID:Addgene_38213 | Firefly Luciferase | Photinus pyralis | Ampicillin | PMID:24357599 | Backbone Size:6616; Vector Backbone:pCENBEVY-U; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin | The first four codons of the original FLuc gene were exchanged for those isoaccepting codons decoded by the least abundant tRNAs in yeast. All remaining codons were exchanged for those isoaccepting codons decoded by the most abundant tRNAs. The last three codons of the full-length Firefly luciferase gene have been deleted to yield a luciferase variant which is fully functional, but no longer localises to peroxisomes. | 2026-08-15 01:14:20 | 0 | |
|
pCDNA3.1Xpress-BcrGAP Resource Report Resource Website |
RRID:Addgene_38175 | BCR | Homo sapiens | Ampicillin | PMID:17116687 | Constructed by Young Jin Cho. A 1.2 kb BamHI xEcoRI fragment from BcrGAP in pGEX3x (AddGene #36405) was inserted into pcDNA BamHI xEcoRI. Contains natural TAG and 3’ UT sequences. The construct includes amino acid residues 1004-1271. | Backbone Size:6700; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | contains GAP domain amino acids 1004-1271 | 2026-08-15 01:14:19 | 0 |
|
pCDNA3.1Xpress-BcrGAP R1090A Resource Report Resource Website |
RRID:Addgene_38176 | BCR GAP domain encoding residues 1004-1271 with R1090A point mutation | Homo sapiens | Ampicillin | PMID:17116687 | Constructed by Young Jin Cho. pcDNA3.1Xpress-BcrGAP plasmid(AddGene #38175) was digested by BstEII x EcoRI and a 1.2 kb fragment containing the R1090 wt site, TAG and 3’ UT sequences removed. The wt fragment was replaced by a 0.6 kb BstEII x EcoRI fragment containing the R1090A mutation isolated from EGFP-BCR R1090A pEGFP (AddGene #38173). The construct contains the natural TAG and 3’ UT sequences. The difference between wt and mutant plasmids is in the 3’ UT region. The construct includes amino acid residues 1004-1271. | Backbone Size:5500; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | aa 1004-1271 with R1090A point mutation | 2026-08-15 01:14:19 | 0 |
|
Abr DH domain NE/AA pGEX Resource Report Resource Website |
RRID:Addgene_38182 | ABR DH domain N282AE283A | Homo sapiens | Ampicillin | PMID:17116687 | Constructed by Young Jin Cho. Mutagenesis done using Stratagene Quick Change kit. 0.35 kb Bgl IIxSstI fragment containing the N282A/E283A double mutation from Abr EYFP (AddGene #36419) was ligated into the "ABR DH domain pGEX" plasmid (AddGene #38181) digested with Bgl II x SstI and removal of the 0.35 kb fragment containing the wt sequence N282E283. The amino acid numbering is as in NM_021962 (isoform A). | Backbone Size:5000; Vector Backbone:pGEX 3X; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | ABR DH domain with N282A and E283A mutations | 2026-08-15 01:14:20 | 0 |
|
pBad-Xis/Int Reset Flipper Resource Report Resource Website 1+ mentions |
RRID:Addgene_38207 | Bxb1-Excisionase | Chloramphenicol | PMID:22615351 | In the reset circuit, PBAD controls expression of a polycistron encoding excisionase with a strong RBS followed by Bxb1 integrase with a GTG start codon to decrease its translation rate and increase the excisionase to integrase ratio. This construct was cloned in a plasmid with a regulated ColE1 origin of replication (J64100, 50-70 copies, chloramphenicol resistance). | Backbone Size:2800; Vector Backbone:J64100; Vector Types:Synthetic Biology, BioBrick cloning vector; Bacterial Resistance:Chloramphenicol | 2026-08-15 01:14:20 | 1 | ||
|
pBad-Int Set Flipper Resource Report Resource Website |
RRID:Addgene_38209 | Bxb1 Integrase | Kanamycin | PMID:22615351 | The Bxb1 integrase was cloned downstream of the PBAD/AraC promoter (BBa_I0500) on pSB3K1 plasmid bearing a p15A origin of replication (15-20 copies). This version of the integrase has a 6-His-tag which we found to stabilize the protein. Therefore, a weak RBS (BBa_B0031) and a LAA ssrA tag was added to reduce the basal expression of the enzyme. | Vector Backbone:pSB3K1; Vector Types:Synthetic Biology, BioBrick cloning vector; Bacterial Resistance:Kanamycin | 2026-08-15 01:14:20 | 0 | ||
|
Strain Phi80 JQ929582 Resource Report Resource Website |
RRID:Addgene_38203 | mKate2 | Synthetic | Kanamycin | PMID:22615351 | This strain contains Addgene plasmid 38200 chromosomally integrated at the Phi80 (Haldimann et al., J of Bacteriology 2001) site of DH5alphaZ1 strain. | Vector Backbone:Phi80; Vector Types:Synthetic Biology, Integration vector; Bacterial Resistance:Kanamycin | 2026-08-15 01:14:20 | 0 | |
|
pMXs-IP-EGFP-ULK2(K39T) Resource Report Resource Website |
RRID:Addgene_38206 | Unc-51 like kinase 2 | Mus musculus | Ampicillin | PMID:18443221 | Backbone Marker:Dr. Toshio Kitamura of the University of Tokyo ; Backbone Size:5847; Vector Backbone:pMXs-IP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin | changed Lysine 39 to Threonine | 2026-08-15 01:14:20 | 0 | |
|
Fer in pAcG2T Resource Report Resource Website 1+ mentions |
RRID:Addgene_38167 | FER | Homo sapiens | Ampicillin | Constructed by Leena Haataja. 4-way ligation of BamHI/NsiI + NsiI/KpnI + KpnI/EcoRI fragments into pAcG2T digested with BamHI x EcoRI. The construct makes Fer protein but not from amplified virus stocks. Large-scale yield of protein is relatively low. | Backbone Marker:Pharmingen; Backbone Size:8530; Vector Backbone:pAcG2T; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:14:19 | 1 | ||
|
Phi80 JQ929582 Resource Report Resource Website |
RRID:Addgene_38200 | mKate2 | Synthetic | Kanamycin | PMID:22615351 | This construct has attL and attR sites (LR). To integrate on your favorite E.coli strain, transform in strain containing the pAH123 plasmid (Haldimann et al., J of Bacteriology 2001). Contains a R6K origin of replication, will only replicate in pir2+ strains. | Vector Backbone:Phi80; Vector Types:Synthetic Biology, Integration vector; Bacterial Resistance:Kanamycin | 2026-08-15 01:14:20 | 0 | |
|
pMXs-IP-EGFP-ULK2 Resource Report Resource Website 1+ mentions |
RRID:Addgene_38201 | Unc-51 like kinase 2 | Mus musculus | Ampicillin | PMID:18443221 | Backbone Marker:Dr. Toshio Kitamura of the University of Tokyo ; Backbone Size:5847; Vector Backbone:pMXs-IP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin | 2026-08-15 01:14:20 | 2 | ||
|
Strain Phi80 JQ929581 Resource Report Resource Website |
RRID:Addgene_38202 | mKate2 | Synthetic | Kanamycin | PMID:22615351 | This strain contains Addgene plasmid 38170 chromosomally integrated at the Phi80 (Haldimann et al., J of Bacteriology 2001) site of DH5alphaZ1 strain. | Vector Backbone:Phi80; Vector Types:Synthetic Biology, Integration vector; Bacterial Resistance:Kanamycin | 2026-08-15 01:14:20 | 0 | |
|
Abr in pAcG2T Resource Report Resource Website |
RRID:Addgene_38162 | ABR | Homo sapiens | Ampicillin | Constructed by Leena Haataja. 4-way ligation of BamHI/StuI + StuI/BstEII + BstEII/EcoRI fragments into pAcG2T digested with BamHI x EcoRI. The construct makes Abr protein. Addgene sequencing found an E24G point mutation--this does not alter plasmid function. | Backbone Marker:Pharmingen; Backbone Size:8530; Vector Backbone:pAcG2T; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:14:19 | 0 | ||
|
Bcr in pAcG2T Resource Report Resource Website |
RRID:Addgene_38163 | BCR | Homo sapiens | Ampicillin | Constructed by Vesa Kaartinen. Makes Bcr protein. Nucleotide sequence from BamHI cloning site to BCR ATG is: 5' GGA TCC CCC GGG CTG CAG GAA TTC GAT GGC CGC GCC ATG | Backbone Marker:Pharmingen; Backbone Size:8530; Vector Backbone:pAcG2T; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:14:19 | 0 | ||
|
Rac1 in pAcG2T Resource Report Resource Website |
RRID:Addgene_38164 | Rac1 | Homo sapiens | Ampicillin | Constructed by Nora Heisterkamp. Rac1 cDNA isolated from K562 cDNA library. 5' BamHI site introduced by PCR. Constructed in 3-way ligation: 0.18 kb 5' BamHI-PvuII + 3' 0.4 kb PvuII-EcoRI into pAcG2T. Makes Rac1 in insect cells. | Backbone Marker:Pharmingen; Backbone Size:8530; Vector Backbone:pAcG2T; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:14:19 | 0 | ||
|
pEF6.HA-ARRDC3 Resource Report Resource Website 1+ mentions |
RRID:Addgene_38317 | ARRDC3 | Homo sapiens | Ampicillin | PMID:20559325 | Backbone Marker:Invitrogen; Vector Backbone:pEF6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:14:21 | 1 | ||
|
pEF6.mCherry-TSG101 Resource Report Resource Website 10+ mentions |
RRID:Addgene_38318 | TSG101 | Homo sapiens | Ampicillin | PMID:22315426 | Please note that there is a mutation in the TSG101 ORF, Y82H. This mutation should not affect plasmid function. | Backbone Marker:Invitrogen; Backbone Size:5780; Vector Backbone:pEF6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:14:21 | 15 | |
|
pCI-neo.ARRDC1-mCherry Resource Report Resource Website |
RRID:Addgene_38319 | ARRDC1 | Homo sapiens | Ampicillin | PMID:22315426 | Backbone Marker:Promega; Backbone Size:5472; Vector Backbone:pCI-neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:14:21 | 0 |
Can't find your Plasmid?
We recommend that you click next to the search bar to check some helpful tips on searches and refine your search firstly. If you want to find a specific plasmid, it's easier to enter an RRID or an Addgene Catalog Number to search. You can refine the search results using Facets on the left side of the search results page. If you are on the table view, you can also search in a specific column by clicking the column title and enter the keywords.
If you still could not find your plasmid in the search results, please help us by registering it into the system — it's easy. Register it with Addgene.
Welcome to the dkNET Resources search. From here you can search through a compilation of resources used by dkNET and see how data is organized within our community.
You are currently on the Community Resources tab looking through categories and sources that dkNET has compiled. You can navigate through those categories from here or change to a different tab to execute your search through. Each tab gives a different perspective on data.
If you have an account on dkNET then you can log in from here to get additional features in dkNET such as Collections, Saved Searches, and managing Resources.
Here is the search term that is being executed, you can type in anything you want to search for. Some tips to help searching:
If you are logged into dkNET you can add data records to your collections to create custom spreadsheets across multiple sources of data.
Here are the facets that you can filter the data by.
If you have any further questions please check out our FAQs Page to ask questions and see our tutorials. Click this button to view this tutorial again.
The NIDDK Information Network (dkNET) serves the needs of basic and clinical investigators by providing seamless access to large pools of data and research resources relevant to the mission of The National Institute of Diabetes Digestive and Kidney Diseases (NIDDK). dkNET is hosted at University of California San Diego, and is supported by NIH NIDDK grant U24DK097771.
Email: info_at_ dknet.org
