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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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p1177-X1-8 Resource Report Resource Website |
RRID:Addgene_38150 | Carboxypeptidase A | M. anisopliae | Ampicillin | PMID:21073956 | Backbone Marker:Invitrogen; Backbone Size:6526; Vector Backbone:pFastBac-1; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:14:19 | 0 | ||
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pcDNA4-FLAG-Jhdm2a Resource Report Resource Website 1+ mentions |
RRID:Addgene_38136 | Jhdm2a | Mus musculus | Ampicillin | PMID:22722204 | Backbone Size:5100; Vector Backbone:pcDNA4/myc-His A; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | protein starts at aa115 | 2026-08-15 01:14:19 | 3 | |
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pEGFP-N1-MITF-A Resource Report Resource Website 1+ mentions |
RRID:Addgene_38132 | MITF-A | Homo sapiens | Kanamycin | PMID:22692423 | Backbone Marker:Clontech; Backbone Size:4733; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | deleted stop codon | 2026-08-15 01:14:19 | 9 | |
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pCW57.1-4EBP1_4xAla Resource Report Resource Website 10+ mentions |
RRID:Addgene_38240 | 4EBP1 | Rattus norvegicus | Ampicillin | PMID:22552098 | Backbone Marker:Broad Insitute; Backbone Size:5000; Vector Backbone:pCW57.1; Vector Types:Mammalian Expression, Lentiviral, Doxycycline inducible; Bacterial Resistance:Ampicillin | T37A, T46A, S65A, T70A (dominant negative) | 2026-08-15 01:14:20 | 15 | |
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pGE-mSmo-YFP Resource Report Resource Website |
RRID:Addgene_38094 | Smo | Mus musculus | Kanamycin | PMID:12414725 | Vector Backbone:pGE; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:14:19 | 0 | ||
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pIS1-Vim/Actb5UTR-renilla Resource Report Resource Website |
RRID:Addgene_38238 | Vim promoter with Actb 5UTR/Renilla | Mus musculus | Ampicillin | PMID:22552098 | This vector originally contained the mouse Actb 5' UTR followed by the renilla luciferase ORF. In the current version, the promoter and first 30 nt of the 5' UTR have been replaced by the mouse Vim promoter and first 30 nt of the Vim 5' UTR, respectively. | Backbone Size:4000; Vector Backbone:pIS1; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin | Actb promoter (1000 nt upstream) and 5' UTR was replaced with the Vim promoter and first 30 nt of Actb 5' UTR | 2026-08-15 01:14:20 | 0 |
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pEBB HA cIAP1 H588A Resource Report Resource Website 1+ mentions |
RRID:Addgene_38233 | cIAP1 H588A | Homo sapiens | Ampicillin | PMID:19243308 | Backbone Marker:Baltimore Lab; Backbone Size:5350; Vector Backbone:pEBB; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | H588A | 2026-08-15 01:14:20 | 1 | |
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pMXs-IP-EGFP-LC3 Resource Report Resource Website 1+ mentions |
RRID:Addgene_38195 | microtubule-associated protein 1 light chain 3 beta | Rattus norvegicus | Ampicillin | PMID:18443221 | Backbone Marker:Dr. Toshio Kitamura of the University of Tokyo ; Backbone Size:5847; Vector Backbone:pMXs-IP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin | 2026-08-15 01:14:20 | 2 | ||
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pMXs-IP-EGFP-ULK1(K46N) Resource Report Resource Website 1+ mentions |
RRID:Addgene_38197 | a novel protein kinase structurally related to C. elegans UNC-51 | Mus musculus | Ampicillin | PMID:18443221 | Backbone Marker:Dr. Toshio Kitamura of the University of Tokyo ; Backbone Size:5847; Vector Backbone:pMXs-IP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin | changed Lysine 46 to Asparagine | 2026-08-15 01:14:20 | 2 | |
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pMXs-IP-EGFP-ULK1(1-828) Resource Report Resource Website |
RRID:Addgene_38198 | a novel protein kinase structurally related to C. elegans UNC-51 | Mus musculus | Ampicillin | PMID:18443221 | Backbone Marker:Dr. Toshio Kitamura of the University of Tokyo ; Backbone Size:5847; Vector Backbone:pMXs-IP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin | deleted amino acids 829-1051 | 2026-08-15 01:14:20 | 0 | |
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pTH736-CEN-staHIS3 Resource Report Resource Website |
RRID:Addgene_38228 | HIS3 | Saccharomyces cerevisiae | Ampicillin | PMID:24357599 | Backbone Size:6614; Vector Backbone:pCENBEVY-U; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:14:20 | 0 | ||
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HA-tagged Bcr Resource Report Resource Website 1+ mentions |
RRID:Addgene_38189 | BCR | Homo sapiens | Ampicillin | Constructed by Young Jin Cho. A human BCR cDNA was inserted into pKH3 (Addgene plasmid #12555; obtained from Martin Schwartz) digested with BamHI x EcoRI in a three-way ligation (1.7 kb BamHI-SstI + 2.6 kb SstI-EcoRI). The 1.7 kb BamHI-SstI fragment was isolated from Bcr in pAcG2T (AddGene plasmid #38163). The 5’ sequence is GGA TCC CCC GGG CTG CAG GAA TTC GAT GGC CGC GCC ATG-start BCR. | Backbone Size:4805; Vector Backbone:pKH3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:14:20 | 2 | ||
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pTH743-CEN-RLuc/slowstaCFLuc Resource Report Resource Website |
RRID:Addgene_38223 | Firefly Luciferase | Photinus pyralis | Ampicillin | PMID:24357599 | Backbone Size:7122; Vector Backbone:pCENBEVY-U; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin | The last three codons of the full-length Firefly luciferase gene have been deleted to yield a luciferase variant which is fully functional, but no longer localises to peroxisomes. | 2026-08-15 01:14:20 | 0 | |
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BCR/ABL P190 transgenic construct Resource Report Resource Website 1+ mentions |
RRID:Addgene_38185 | BCR/ABL P190 | Homo sapiens | Ampicillin | PMID:2179728 | The DNA construct was made by Guido Jenster and Nora Heisterkamp in several cloning and subcloning steps. The transgenic construct contains human BCR exon 1, intron sequences of human BCR and ABL genes, human ABL exon 2 and ABL cDNA sequences. The transgene expression is controlled by a 200-bp Sst I- Bgl II fragment of the murine metallothionein-1 (Mt1) promoter lacking glucocorticoid-responsive elements (PMID 8541551). This BCR/ABL fusion gene produces a P190 protein and is a model for Philadelphia chromosome-positive acute lymphoblastic leukemia (ALL) in humans, which is caused by a chromosomal translocation that fuses the ABL proto-oncogene with breakpoint cluster region (BCR) sequences. Leukemia development is relatively rapid on inbred C57Bl/6J and CBA backgrounds and is slower on mixed genetic backgrounds. Mice transgenic for this construct on an inbred C57Bl/6J background are also available from Jackson labs stock 017833. The insert can be separated from the vector by digestion with Sst II. | Backbone Size:3000; Vector Backbone:pSK; Vector Types:construct for making transgenic mice; Bacterial Resistance:Ampicillin | 2026-08-15 01:14:20 | 3 | |
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human CRKL transgenic construct Resource Report Resource Website |
RRID:Addgene_38186 | CRKL | Homo sapiens | Ampicillin | PMID:11245441 | This construct was made by Arnoud van Wijk. It can be used to make transgenic mice. The DNA construct contains 7.5 kb of human CRKL locus 5’ sequences including the promoter of the human v-crk sarcoma virus CT10 oncogene homolog (CRKL) gene, joined to CRKL exon 1. Part of the intron between exons 1 and 2 encompassing around 8-kb is included and exons 2-3 as cDNA. The entire insert is around 17 kb and can be isolated free from plasmid sequences in a Kpn I x Mlu I digestion. Jackson labs stock no. 017834 is one of our CRKL transgenics containing approximately 5 copies of this transgene. CRKL overexpressing transgenics are viable but mice with high copy numbers have reduced fertility. How the CRKL transgenic construct was made: (verbatim from PMID 11245441): Human genomic clone 70 (PMID 7905853) contains 12 kb of 5’ sequences, exon 1, and 6.6 kb of intron 1 of the CRKL gene. A 350-bp RsaI fragment isolated from a CRKL cDNA (GenBank accession no. X59656) was used as a probe to specifically isolate a phage clone, CR-4, containing CRKL exon 2 flanked by 6.5 kb of intron 1 and 9.5 kb of intron 2. CRKL is located on human chromosome 22 and has been entirely sequenced; introns 1 and 2 are both around 15.5 kb. To generate a transgenic DNA construct, a 7-kb SalI-BamHI fragment from CR-4, which included a SalI site at the 5’ end from the phage polylinker and the 3’ BamHI site located in exon 2, was ligated with a 0.78-kb BamHI-EcoRI cDNA fragment, including exons 2, 3, and the 3’ untranslated region, into pSK digested with SalIxEcoRI. The insert was removed as a 7.7-kb SalI-NotI fragment and ligated with a 1.2-kb SstII-SalI fragment from intron 1 in clone 70 into pSK digested with SstII x NotI. The resulting insert was removed by digestion with SstII x NotI. The 5’ promoter and exon 1 sequences were isolated on an 8-kb EcoRI-SstII fragment that was subcloned into pSK digested with EcoRI x SstII; the insert was removed as an 8-kb KpnI-SstII fragment. The 8-kb KpnI-SstII fragment plus the 7.7-kb SstII-NotI fragment were ligated into pSL1180 digested with KpnI x NotI. | Backbone Size:3500; Vector Backbone:pSL1180; Vector Types:mouse transgenic; Bacterial Resistance:Ampicillin | Contains human CRKL introns | 2026-08-15 01:14:20 | 0 |
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pTH738-CENBEVYslow Resource Report Resource Website |
RRID:Addgene_38220 | GCN4-based single uORF-containing 5'-UTR | Saccharomyces cerevisiae | Ampicillin | PMID:24357599 | Backbone Size:6614; Vector Backbone:pCENBEVY-U; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin | The start codons of uORFs2, 3 and4 of the yeast Gcn4 leader sequence were mutated to non-start codons. | 2026-08-15 01:14:20 | 0 | |
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pMXs-IP-EGFP-hAtg13 Resource Report Resource Website 1+ mentions |
RRID:Addgene_38191 | autophagy-related genes 13 | Homo sapiens | Ampicillin | PMID:19211835 | Backbone Marker:Dr. Toshio Kitamura of the University of Tokyo ; Backbone Size:5847; Vector Backbone:pMXs-IP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin | 2026-08-15 01:14:20 | 2 | ||
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pMXs-puro-EGFP-FAK Resource Report Resource Website 1+ mentions |
RRID:Addgene_38194 | PTK2 protein tyrosine kinase 2 | Mus musculus | Ampicillin | PMID:18443221 | Backbone Marker:Dr. Toshio Kitamura of the University of Tokyo ; Backbone Size:6878; Vector Backbone:pMXs-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin | 2026-08-15 01:14:20 | 3 | ||
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HA-tagged Abr Resource Report Resource Website |
RRID:Addgene_38190 | ABR | Homo sapiens | Ampicillin | Constructed by Young Jin Cho. A human ABR cDNA was inserted into pKH3 (Addgene plasmid 12555; obtained from Martin Schwartz) digested with BamHI x EcoRI in a three-way ligation (0.4 kb BamHI-BstEII + 2.2 kb BstEII-EcoRI). The 5’ sequence is GGA TCC ATG-start ABR. Note: This plasmid may contain an E24G mutation -gag to ggg- introduced by Taq polymerase at an earlier cloning step. | Backbone Size:4805; Vector Backbone:pKH3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:14:20 | 0 | ||
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pTH753-CEN-RLuc/min346maxCFLuc Resource Report Resource Website |
RRID:Addgene_38219 | Firefly Luciferase | Photinus pyralis | Ampicillin | PMID:24357599 | Backbone Size:6616; Vector Backbone:pCENBEVY-U; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin | The first 346 codons of the original FLuc gene were exchanged for those isoaccepting codons decoded by the least abundant tRNAs in yeast. All remaining codons were exchanged for those isoaccepting codons decoded by the most abundant tRNAs. The last three codons of the full-length Firefly luciferase gene have been deleted to yield a luciferase variant which is fully functional, but no longer localises to peroxisomes. | 2026-08-15 01:14:20 | 0 |
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