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Species: M. anisopliae
Genetic Insert: Carboxypeptidase A
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:6526; Vector Backbone:pFastBac-1; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21073956
Proper citation: RRID:Addgene_38150 Copy
Species: Mus musculus
Genetic Insert: Jhdm2a
Vector Backbone Description: Backbone Size:5100; Vector Backbone:pcDNA4/myc-His A; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22722204
Proper citation: RRID:Addgene_38136 Copy
Species: Homo sapiens
Genetic Insert: MITF-A
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4733; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22692423
Proper citation: RRID:Addgene_38132 Copy
Species: Rattus norvegicus
Genetic Insert: 4EBP1
Vector Backbone Description: Backbone Marker:Broad Insitute; Backbone Size:5000; Vector Backbone:pCW57.1; Vector Types:Mammalian Expression, Lentiviral, Doxycycline inducible; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22552098
Proper citation: RRID:Addgene_38240 Copy
Species: Mus musculus
Genetic Insert: Smo
Vector Backbone Description: Vector Backbone:pGE; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:12414725
Proper citation: RRID:Addgene_38094 Copy
Species: Mus musculus
Genetic Insert: Vim promoter with Actb 5UTR/Renilla
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pIS1; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22552098
Comments: This vector originally contained the mouse Actb 5' UTR followed by the renilla luciferase ORF. In the current version, the promoter and first 30 nt of the 5' UTR have been replaced by the mouse Vim promoter and first 30 nt of the Vim 5' UTR, respectively.
Proper citation: RRID:Addgene_38238 Copy
Species: Homo sapiens
Genetic Insert: cIAP1 H588A
Vector Backbone Description: Backbone Marker:Baltimore Lab; Backbone Size:5350; Vector Backbone:pEBB; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19243308
Proper citation: RRID:Addgene_38233 Copy
Species: Rattus norvegicus
Genetic Insert: microtubule-associated protein 1 light chain 3 beta
Vector Backbone Description: Backbone Marker:Dr. Toshio Kitamura of the University of Tokyo ; Backbone Size:5847; Vector Backbone:pMXs-IP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18443221
Proper citation: RRID:Addgene_38195 Copy
Species: Mus musculus
Genetic Insert: a novel protein kinase structurally related to C. elegans UNC-51
Vector Backbone Description: Backbone Marker:Dr. Toshio Kitamura of the University of Tokyo ; Backbone Size:5847; Vector Backbone:pMXs-IP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18443221
Proper citation: RRID:Addgene_38197 Copy
Species: Mus musculus
Genetic Insert: a novel protein kinase structurally related to C. elegans UNC-51
Vector Backbone Description: Backbone Marker:Dr. Toshio Kitamura of the University of Tokyo ; Backbone Size:5847; Vector Backbone:pMXs-IP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18443221
Proper citation: RRID:Addgene_38198 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: HIS3
Vector Backbone Description: Backbone Size:6614; Vector Backbone:pCENBEVY-U; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24357599
Proper citation: RRID:Addgene_38228 Copy
Species: Homo sapiens
Genetic Insert: BCR
Vector Backbone Description: Backbone Size:4805; Vector Backbone:pKH3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Constructed by Young Jin Cho.
A human BCR cDNA was inserted into pKH3 (Addgene plasmid #12555; obtained from Martin Schwartz) digested with BamHI x EcoRI in a three-way ligation (1.7 kb BamHI-SstI + 2.6 kb SstI-EcoRI). The 1.7 kb BamHI-SstI fragment was isolated from Bcr in pAcG2T (AddGene plasmid #38163). The 5’ sequence is
GGA TCC CCC GGG CTG CAG GAA TTC GAT GGC CGC GCC ATG-start BCR.
Proper citation: RRID:Addgene_38189 Copy
Species: Photinus pyralis
Genetic Insert: Firefly Luciferase
Vector Backbone Description: Backbone Size:7122; Vector Backbone:pCENBEVY-U; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24357599
Proper citation: RRID:Addgene_38223 Copy
Species: Homo sapiens
Genetic Insert: BCR/ABL P190
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pSK; Vector Types:construct for making transgenic mice; Bacterial Resistance:Ampicillin
Defining Citation: PMID:2179728
Comments: The DNA construct was made by Guido Jenster and Nora Heisterkamp in several cloning and subcloning steps. The transgenic construct contains human BCR exon 1, intron sequences of human BCR and ABL genes, human ABL exon 2 and ABL cDNA sequences. The transgene expression is controlled by a 200-bp Sst I- Bgl II fragment of the murine metallothionein-1 (Mt1) promoter lacking glucocorticoid-responsive elements (PMID 8541551). This BCR/ABL fusion gene produces a P190 protein and is a model for Philadelphia chromosome-positive acute lymphoblastic leukemia (ALL) in humans, which is caused by a chromosomal translocation that fuses the ABL proto-oncogene with breakpoint cluster region (BCR) sequences. Leukemia development is relatively rapid on inbred C57Bl/6J and CBA backgrounds and is slower on mixed genetic backgrounds. Mice transgenic for this construct on an inbred C57Bl/6J background are also available from Jackson labs stock 017833. The insert can be separated from the vector by digestion with Sst II.
Proper citation: RRID:Addgene_38185 Copy
Species: Homo sapiens
Genetic Insert: CRKL
Vector Backbone Description: Backbone Size:3500; Vector Backbone:pSL1180; Vector Types:mouse transgenic; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11245441
Comments: This construct was made by Arnoud van Wijk. It can be used to make transgenic mice. The DNA construct contains 7.5 kb of human CRKL locus 5’ sequences including the promoter of the human v-crk sarcoma virus CT10 oncogene homolog (CRKL) gene, joined to CRKL exon 1. Part of the intron between exons 1 and 2 encompassing around 8-kb is included and exons 2-3 as cDNA. The entire insert is around 17 kb and can be isolated free from plasmid sequences in a Kpn I x Mlu I digestion. Jackson labs stock no. 017834 is one of our CRKL transgenics containing approximately 5 copies of this transgene. CRKL overexpressing transgenics are viable but mice with high copy numbers have reduced fertility.
How the CRKL transgenic construct was made: (verbatim from PMID 11245441): Human genomic clone 70 (PMID 7905853) contains 12 kb of 5’ sequences, exon 1, and 6.6 kb of intron 1 of the CRKL gene. A 350-bp RsaI fragment isolated from a CRKL cDNA (GenBank accession no. X59656) was used as a probe to specifically isolate a phage clone, CR-4, containing CRKL exon 2 flanked by 6.5 kb of intron 1 and 9.5 kb of intron 2. CRKL is located on human chromosome 22 and has been entirely sequenced; introns 1 and 2 are both around 15.5 kb. To generate a transgenic DNA construct, a 7-kb SalI-BamHI fragment from CR-4, which included a SalI site at the 5’ end from the phage polylinker and the 3’ BamHI site located in exon 2, was ligated with a 0.78-kb BamHI-EcoRI cDNA fragment, including exons 2, 3, and the 3’ untranslated region, into pSK digested with SalIxEcoRI. The insert was removed as a 7.7-kb SalI-NotI fragment and ligated with a 1.2-kb SstII-SalI fragment from intron 1 in clone 70 into pSK digested with SstII x NotI. The resulting insert was removed by digestion with SstII x NotI. The 5’ promoter and exon 1 sequences were isolated on an 8-kb EcoRI-SstII fragment that was subcloned into pSK digested with EcoRI x SstII; the insert was removed as an 8-kb KpnI-SstII fragment. The 8-kb KpnI-SstII fragment plus the 7.7-kb SstII-NotI fragment were ligated into pSL1180 digested with KpnI x NotI.
Proper citation: RRID:Addgene_38186 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: GCN4-based single uORF-containing 5'-UTR
Vector Backbone Description: Backbone Size:6614; Vector Backbone:pCENBEVY-U; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24357599
Proper citation: RRID:Addgene_38220 Copy
Species: Homo sapiens
Genetic Insert: autophagy-related genes 13
Vector Backbone Description: Backbone Marker:Dr. Toshio Kitamura of the University of Tokyo ; Backbone Size:5847; Vector Backbone:pMXs-IP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19211835
Proper citation: RRID:Addgene_38191 Copy
Species: Mus musculus
Genetic Insert: PTK2 protein tyrosine kinase 2
Vector Backbone Description: Backbone Marker:Dr. Toshio Kitamura of the University of Tokyo ; Backbone Size:6878; Vector Backbone:pMXs-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18443221
Proper citation: RRID:Addgene_38194 Copy
Species: Homo sapiens
Genetic Insert: ABR
Vector Backbone Description: Backbone Size:4805; Vector Backbone:pKH3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Constructed by Young Jin Cho.
A human ABR cDNA was inserted into pKH3 (Addgene plasmid 12555; obtained from Martin Schwartz) digested with BamHI x EcoRI in a three-way ligation (0.4 kb BamHI-BstEII + 2.2 kb BstEII-EcoRI). The 5’ sequence is
GGA TCC ATG-start ABR. Note: This plasmid may contain an E24G mutation -gag to ggg- introduced by Taq polymerase at an earlier cloning step.
Proper citation: RRID:Addgene_38190 Copy
Species: Photinus pyralis
Genetic Insert: Firefly Luciferase
Vector Backbone Description: Backbone Size:6616; Vector Backbone:pCENBEVY-U; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24357599
Proper citation: RRID:Addgene_38219 Copy
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