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Species: Synthetic
Genetic Insert: 3xFLAG-dCas9
Vector Backbone Description: Backbone Marker:Takara Bio; Backbone Size:6452; Vector Backbone:pMSCVpuro; Vector Types:Mammalian Expression, Retroviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34409168
Comments: Construction strategy of gRNA retroviral vectors
1. Cleave gBlock from a gRNA vector constructed using gRNA cloning vector (Addgene #41824) with appropriate restriction enzymes (eg. [Xho I + Hind III], EcoR I).
2. Insert the cleaved gBlock into pSIR-based self-inactivating retroviral vectors.
Vectors & Sites of insertion
pSIR-neo (Addgene #51128): eg. [Xho I + Hind III]
pSIR-GFP (Addgene #51134): eg. [Xho I + Hind III], EcoR I
pSIR-DsRed-Express2 (Addgene #51135): eg. [Xho I + Hind III], EcoR I
pSIR-hCD2 (Addgene #51143): eg. EcoR I
Proper citation: RRID:Addgene_134983 Copy
Species: Homo sapiens
Genetic Insert: m.5147-Left TALE-G1397-N-dead-mCherry
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Mammalian Expression, TALEN; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_211341 Copy
Species: Homo sapiens
Genetic Insert: sgRNA targeting human AGER locus
Vector Backbone Description: Backbone Marker:Morrissey Laboratory; Backbone Size:9274; Vector Backbone:PX458; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38642558
Comments: Please visit https://www.biorxiv.org/content/10.1101/2023.01.19.524655v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_216473 Copy
Species: S. aureus
Genetic Insert: DNA gyrase
Vector Backbone Description: Backbone Marker:Thermo; Backbone Size:5800; Vector Backbone:pET101; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38578824
Proper citation: RRID:Addgene_216230 Copy
Species: Homo sapiens
Genetic Insert: sgRNA targeting human AGER locus
Vector Backbone Description: Backbone Marker:Morrissey Laboratory; Backbone Size:9274; Vector Backbone:PX458; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38642558
Comments: Please visit https://www.biorxiv.org/content/10.1101/2023.01.19.524655v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_216472 Copy
Species: Aequorea victoria
Genetic Insert: eGFP
Vector Backbone Description: Backbone Marker:Darrell Kotton; Backbone Size:7674; Vector Backbone:p2701-AGER-tdTomato; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38642558
Comments: Please visit https://www.biorxiv.org/content/10.1101/2023.01.19.524655v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_216474 Copy
Species: Discosoma coral
Genetic Insert: dsRed
Vector Backbone Description: Vector Backbone:pHAGE2; Vector Types:Lentiviral, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38642558
Comments: Please visit https://www.biorxiv.org/content/10.1101/2023.01.19.524655v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_216477 Copy
Species: S. aureus
Genetic Insert: DNA gyrase
Vector Backbone Description: Backbone Marker:Thermo; Backbone Size:5800; Vector Backbone:pET101; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38578824
Proper citation: RRID:Addgene_216234 Copy
Species: Homo sapiens
Genetic Insert: m.3075-Right Puro DdCBE-G1397-C- T1413I-GFP
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Mammalian Expression, TALEN; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_211343 Copy
Species: Sars Cov 2
Genetic Insert: Sars Cov 2 Mac Domain
Vector Backbone Description: Vector Backbone:pNIC28-Bse4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: https://asapdiscovery.org/outputs/target-enabling-packages/
Proper citation: RRID:Addgene_204787 Copy
Species: Homo sapiens
Genetic Insert: NAP1
Vector Backbone Description: Vector Backbone:pBMN; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_208868 Copy
Species: Other
Genetic Insert: CLV3p::spCas9::PEA3At::U6p::AAVS1gRNA::pHTR5::LT0::mCherrySSM
Vector Backbone Description: Vector Backbone:pSUN; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37403571
Proper citation: RRID:Addgene_210548 Copy
Species: Homo sapiens
Genetic Insert: OPTN
Vector Backbone Description: Vector Backbone:pHAGE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_208867 Copy
Species: Homo sapiens
Genetic Insert: NAP1
Vector Backbone Description: Vector Backbone:pGEX-4T1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_217610 Copy
Species: Homo sapiens
Genetic Insert: HaloTag followed by a PolyA signal and PuroR cassette flanked by human ULK1 locus sequences
Vector Backbone Description: Backbone Size:5500; Vector Backbone:pFastBac dual; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37115157
Proper citation: RRID:Addgene_207548 Copy
Species: Homo sapiens
Genetic Insert: TBK1
Vector Backbone Description: Vector Backbone:pFastBac_Dual; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_208875 Copy
Species: Homo sapiens
Genetic Insert: NAP1
Vector Backbone Description: Vector Backbone:pGEX-4T1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_208872 Copy
Species: Homo sapiens
Genetic Insert: HaloTag flanked by human ATG5 locus sequences
Vector Backbone Description: Backbone Size:5500; Vector Backbone:pFastBac dual; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37115157
Proper citation: RRID:Addgene_207542 Copy
Vector Backbone Description: Vector Backbone:CROPseq-Guide-BSD (#216123); Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38641660
Proper citation: RRID:Addgene_216134 Copy
Species: other
Genetic Insert: BBa_J23105-cas9 + pBAD-λ Red genes + ptet-gRNA (pBR322ori)
Vector Backbone Description: Vector Backbone:oriR101; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38086386
Proper citation: RRID:Addgene_214751 Copy
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