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Species: Homo sapiens
Genetic Insert: FKBP1A
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pmRFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22722250
Comments: Compared to GenBank reference sequence NM_019892, the INPP5E insert in this plasmid contains a C641A mutation. Please see the full plasmid sequence for the most accurate information.
Proper citation: RRID:Addgene_38001 Copy
Species: Homo sapiens
Genetic Insert: YTHDF2 YTH domain family, member 2
Vector Backbone Description: Backbone Size:5496; Vector Backbone:pFRT/TO/HIS/FLAG/HA-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22681889
Proper citation: RRID:Addgene_38089 Copy
Species: Homo sapiens
Genetic Insert: PES1 pescadillo ribosomal biogenesis factor 1
Vector Backbone Description: Backbone Size:5490; Vector Backbone:pFRT/TO/HIS/FLAG/HA-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22681889
Proper citation: RRID:Addgene_38085 Copy
Species: Homo sapiens
Genetic Insert: PRDX1 peroxiredoxin 1
Vector Backbone Description: Backbone Size:5321; Vector Backbone:pFRT/TO/HIS/FLAG/HA-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22681889
Proper citation: RRID:Addgene_38086 Copy
Species: Homo sapiens
Genetic Insert: FKBP1A
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pmRFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22722250
Comments: Compared to GenBank reference sequence NM_019892, the INPP5E insert in this plasmid contains a C641A mutation. Please see the full plasmid sequence for the most accurate information.
Proper citation: RRID:Addgene_38000 Copy
Species: H. Herpesvirus 4 (EBV)
Genetic Insert: EBNA3C
Vector Backbone Description: Backbone Size:8162; Vector Backbone:MSCV-N FLAGHA; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22810586
Comments: Note that the Gateway cloning reaction has left several vector-based nucleotides in frame with the 3' end of the insert, adding several amino acid residues [FLYKVVR] fused to the C-terminus of translated protein.
Proper citation: RRID:Addgene_37958 Copy
Species: H. Herpesvirus 4 (EBV)
Genetic Insert: BRRF1
Vector Backbone Description: Backbone Size:8162; Vector Backbone:MSCV-N FLAGHA; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22810586
Comments: Note that the Gateway cloning reaction has left several vector-based nucleotides in frame with the 3' end of the insert, leaving additional amino acid residues [PTFLYKVVR] fused to the C-terminus of translated protein.
Proper citation: RRID:Addgene_37943 Copy
Species: H. Herpesvirus 4 (EBV)
Genetic Insert: BRRF2
Vector Backbone Description: Backbone Size:8162; Vector Backbone:MSCV-N FLAGHA; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22810586
Comments: Note that the Gateway cloning reaction has left several vector-based nucleotides in frame with the 3' end of the insert, leaving additional amino acid residues [PTFLYKVVR] fused to the C-terminus of translated protein.
Proper citation: RRID:Addgene_37944 Copy
Species: H. Herpesvirus 4 (EBV)
Genetic Insert: BLRF2
Vector Backbone Description: Backbone Size:8162; Vector Backbone:MSCV-N FLAGHA; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22810586
Comments: Note that the Gateway cloning reaction has left several vector-based nucleotides in frame with the 3' end of the insert, leaving additional amino acid residues [PTFLYKVVR] fused to the C-terminus of translated protein.
Proper citation: RRID:Addgene_37940 Copy
Species: H. Herpesvirus 4 (EBV)
Genetic Insert: BNLF2a
Vector Backbone Description: Backbone Size:8162; Vector Backbone:MSCV-N FLAGHA; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22810586
Comments: Note that the Gateway cloning reaction has left several vector-based nucleotides in frame with the 3' end of the insert, leaving additional amino acid residues [PTFLYKVVR] fused to the C-terminus of translated protein.
Proper citation: RRID:Addgene_37941 Copy
Species: H. Herpesvirus 4 (EBV)
Genetic Insert: BNLF2b
Vector Backbone Description: Backbone Size:8162; Vector Backbone:MSCV-N FLAGHA; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22810586
Comments: Note that the Gateway cloning reaction has left several vector-based nucleotides in frame with the 3' end of the insert, leaving additional amino acid residues [PTFLYKVVR] fused to the C-terminus of translated protein.
Proper citation: RRID:Addgene_37942 Copy
Species: Homo sapiens
Genetic Insert: EDF1 endothelial differentiation-related factor 1
Vector Backbone Description: Backbone Size:5496; Vector Backbone:pFRT/TO/HIS/FLAG/HA-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22681889
Proper citation: RRID:Addgene_38079 Copy
Species: Homo sapiens
Genetic Insert: API5 apoptosis inhibitor 5
Vector Backbone Description: Backbone Size:5496; Vector Backbone:pFRT/TO/HIS/FLAG/HA-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22681889
Proper citation: RRID:Addgene_38074 Copy
Species: Homo sapiens
Genetic Insert: KIAA1967
Vector Backbone Description: Backbone Size:5539; Vector Backbone:pFRT/TO/HIS/FLAG/HA-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22681889
Proper citation: RRID:Addgene_38082 Copy
Species: Homo sapiens
Genetic Insert: MYBBP1A MYB binding protein (P160) 1a
Vector Backbone Description: Backbone Size:5499; Vector Backbone:pFRT/TO/HIS/FLAG/HA-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22681889
Proper citation: RRID:Addgene_38084 Copy
Species: H. Herpesvirus 4 (EBV)
Genetic Insert: BVLF1.5
Vector Backbone Description: Backbone Size:8162; Vector Backbone:MSCV-N FLAGHA; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22810586
Comments: Note that the Gateway cloning reaction has left several vector-based nucleotides in frame with the 3' end of the insert, leaving additional amino acid residues [PTFLYKVVR] fused to the C-terminus of translated protein.
Proper citation: RRID:Addgene_37949 Copy
Species: Homo sapiens
Genetic Insert: HNRNPR heterogeneous nuclear ribonucleoprotein R
Vector Backbone Description: Backbone Size:5538; Vector Backbone:pFRT/TO/HIS/FLAG/HA-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22681889
Proper citation: RRID:Addgene_38067 Copy
Species: Homo sapiens
Genetic Insert: HNRNPU heterogeneous nuclear ribonucleoprotein U (scaffold attachment factor A)
Vector Backbone Description: Backbone Size:5496; Vector Backbone:pFRT/TO/HIS/FLAG/HA-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22681889
Proper citation: RRID:Addgene_38068 Copy
Species: Homo sapiens
Genetic Insert: MYEF2 myelin expression factor 2
Vector Backbone Description: Backbone Size:5508; Vector Backbone:pFRT/TO/HIS/FLAG/HA-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22681889
Comments: Digest and NGS results suggest that the original Gateway entry vector is still present in the DNA stock from which the bacterial stocks are created. Because the entry vector does not contain a promoter for the insert or selection marker, it should not interfere with function.
To attempt to remove the entry vector, recipient scientists could try to cure the plasmid from isolated colonies. Alternatively, they can linearize the entry vector with BmtI, HpaI or NheI digest, which will not cut the expression vector, and retransform into bacteria.
Proper citation: RRID:Addgene_38069 Copy
Species: Human adenovirus 5
Genetic Insert: E4 orf1
Vector Backbone Description: Backbone Size:8162; Vector Backbone:MSCV-N FLAGHA; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22810586
Comments: Note that the Gateway cloning reaction has left several vector-based nucleotides in frame with the 3' end of the insert, adding several amino acid residues [STFLYKVVR] fused to the C-terminus of translated protein.
Proper citation: RRID:Addgene_38063 Copy
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