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Species: Oncorhynchus tshawytscha, Aequorea victoria
Genetic Insert: mEGFP-P2A-otch25hb4
Vector Backbone Description: Vector Backbone:pcDNA3.1-(-)-Hyg; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_217484 Copy
Species: Oncorhynchus tshawytscha
Genetic Insert: BFP-P2A-otPKRFL
Vector Backbone Description: Vector Backbone:pcDNA3.1-(-)-Zeo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41125964
Proper citation: RRID:Addgene_217485 Copy
Species: Homo sapiens
Genetic Insert: HOXC4
Vector Backbone Description: Vector Backbone:pGAL4-DBD-MCS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38969762
Proper citation: RRID:Addgene_215586 Copy
Species: Homo sapiens
Genetic Insert: Connexin 32-IRES-GCaMP6s
Vector Backbone Description: Vector Backbone:pCW57.1; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39639332
Proper citation: RRID:Addgene_216795 Copy
Species: Homo sapiens
Genetic Insert: HaloTag DKC1
Vector Backbone Description: Backbone Size:5500; Vector Backbone:pHTN HaloTag Promega; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37267110
Proper citation: RRID:Addgene_207578 Copy
Species: Homo sapiens
Genetic Insert: puro resistance cassette flanked by TR locus sequences excluding TR
Vector Backbone Description: Backbone Size:5500; Vector Backbone:pFastBac dual; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37267110
Proper citation: RRID:Addgene_207579 Copy
Species: Synthetic
Genetic Insert: tdMCP-24xGCN4
Vector Backbone Description: Vector Backbone:pcDNA; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36867026
Proper citation: RRID:Addgene_205157 Copy
Species: Homo sapiens
Genetic Insert: Optineurin
Vector Backbone Description: Vector Backbone:pET-Duet1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_216843 Copy
Species: Homo sapiens
Genetic Insert: UCP1
Vector Backbone Description: Vector Backbone:pBM; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37336486
Proper citation: RRID:Addgene_216207 Copy
Species: Synthetic
Genetic Insert: 3xFLAG-dCas9
Vector Backbone Description: Backbone Marker:Takara Bio; Backbone Size:7133; Vector Backbone:pMSCVhyg; Vector Types:Mammalian Expression, Retroviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34409168
Comments: Construction strategy of gRNA retroviral vectors
1. Cleave gBlock from a gRNA vector constructed using gRNA cloning vector (Addgene #41824) with appropriate restriction enzymes (eg. [Xho I + Hind III], EcoR I).
2. Insert the cleaved gBlock into pSIR-based self-inactivating retroviral vectors.
Vectors & Sites of insertion
pSIR-neo (Addgene #51128): eg. [Xho I + Hind III]
pSIR-GFP (Addgene #51134): eg. [Xho I + Hind III], EcoR I
pSIR-DsRed-Express2 (Addgene #51135): eg. [Xho I + Hind III], EcoR I
pSIR-hCD2 (Addgene #51143): eg. EcoR I
Proper citation: RRID:Addgene_134323 Copy
Species: Synthetic
Genetic Insert: Prime editor 2 (PE2) enzyme
Vector Backbone Description: Backbone Marker:Carlon lab (M. Bulcaen); Vector Backbone:lenti-HS-YFP-IRES-ZeoR; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38697102
Proper citation: RRID:Addgene_207352 Copy
Species: Homo sapiens
Genetic Insert: MutL homolog 1
Vector Backbone Description: Backbone Marker:Carlon lab (M. Bulcaen); Vector Backbone:lenti-HS-YFP-IRES-ZeoR; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38697102
Proper citation: RRID:Addgene_207354 Copy
Species: Synthetic
Genetic Insert: RNF2 +41 ngRNA
Vector Backbone Description: Backbone Marker:Carlon lab (M. Bulcaen); Vector Backbone:lentiCRISPRv2_deltaCas9; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38697102
Proper citation: RRID:Addgene_207358 Copy
Species: Synthetic
Genetic Insert: L227R-CFTR + 13 C>A tevopreq1-prime editing guide RNA (epegRNA)
Vector Backbone Description: Backbone Marker:Carlon lab (M. Bulcaen); Vector Backbone:Lenti-RNF2-tevopreQ1-epegRNA+5G>T_EF1a-puroR; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38697102
Proper citation: RRID:Addgene_207357 Copy
Species: S. aureus
Genetic Insert: DNA gyrase
Vector Backbone Description: Backbone Marker:Thermo; Backbone Size:5800; Vector Backbone:pET101; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38578824
Proper citation: RRID:Addgene_216229 Copy
Species: Synthetic
Genetic Insert: L227R-CFTR +15 ngRNA
Vector Backbone Description: Backbone Marker:Carlon lab (M. Bulcaen); Vector Backbone:lentiCRISPRv2_deltaCas9; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38697102
Proper citation: RRID:Addgene_207360 Copy
Species: Homo sapiens
Genetic Insert: m.3047-Left TALE-G1397-N-dead-mCherry
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Mammalian Expression, TALEN; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_211339 Copy
Species: Ramazzottius varieornatus
Genetic Insert: RvLEAMshort
Vector Backbone Description: Backbone Marker:Novagen/Millipore; Backbone Size:5708; Vector Backbone:pET-15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38370693
Comments: Please visit https://www.biorxiv.org/content/10.1101/2024.02.06.579238v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_218122 Copy
Species: Homo sapiens
Genetic Insert: gRNA_hIRF1 promoter #12
Vector Backbone Description: Backbone Marker:Hodaka Fujii; Backbone Size:8183; Vector Backbone:pSIR-hCD2; Vector Types:Mammalian Expression, Retroviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34409168
Proper citation: RRID:Addgene_135392 Copy
Species: Synthetic
Genetic Insert: 3xFLAG-dCas9
Vector Backbone Description: Backbone Marker:Takara Bio; Backbone Size:6624; Vector Backbone:pMSCVneo; Vector Types:Mammalian Expression, Retroviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34409168
Comments: Construction strategy of gRNA retroviral vectors
1. Cleave gBlock from a gRNA vector constructed using gRNA cloning vector (Addgene #41824) with appropriate restriction enzymes (eg. [Xho I + Hind III], EcoR I).
2. Insert the cleaved gBlock into pSIR-based self-inactivating retroviral vectors.
Vectors & Sites of insertion
pSIR-neo (Addgene #51128): eg. [Xho I + Hind III]
pSIR-GFP (Addgene #51134): eg. [Xho I + Hind III], EcoR I
pSIR-DsRed-Express2 (Addgene #51135): eg. [Xho I + Hind III], EcoR I
pSIR-hCD2 (Addgene #51143): eg. EcoR I
Proper citation: RRID:Addgene_134982 Copy
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