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E. coli MEV15
 
Resource Report
Resource Website 		RRID:Addgene_197112 None E. coli MEV15 is engineered to host sesquiterpenoid biosynthetic pathways. Sesquiterpenoid production is achieved by supplying the strain with plasmids encoding terpene cyclase and cytochrome P450s under the control of Marionette promoters (See https://www.addgene.org/kits/marionette-sensor-collection/ and 10.1038/s41589-018-0168-3). Sesquiterpenoid production can be induced by IPTG, vanillic aci, and other inducers controlling cytorhcome P450s. The strain has the upper MEV pathway from pMevT (addgene #17815) inserted into 4418413/4418414, the lower MEV pathway from pMBIS (addgene #17817) and E. coli ispA inserted into 4105665/4105664, the designed redox enzyme array inserteed into 3801913/3801912, and the Marionette cluster from sAJM.1506 (addgene #108254) inserted into 3753777/3752159 of E. coli BL21(DE3)'s genome. The nucleotide numbers are based on NCBI accession # NZ_CP053602. The redox enzyme array consists of fprD/fdxD from Streptomyces avermitilis, fpr/fldA from E. coli, fenr/fer1 from spinach chloroplasts, abd pdr/pdx (camA/camB) from Pseudomonas putida. The Marionette cluster was transferred using phage transduction, resulting in the replacement of nucleotides between 3745758/3839292 by those in the corresponding regions from sAJM.1506 (parent strain: E. coli MG1655), as evident by whole-genome sequencing. Vector Backbone:N/A; Vector Types:; Bacterial Resistance:None Addgene 2026-10-10 02:04:38 0
pDONR223 SARS-CoV-1 NSP2_nostop
 
Resource Report
Resource Website 		RRID:Addgene_168519 NSP2 Other Spectinomycin PMID:37267226 Backbone Marker:Rual et al, Genome Res. 14:2128-2135, 2004.; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Other; Bacterial Resistance:Spectinomycin Addgene 2026-10-10 02:04:38 0
pDONR223 HCoV-HKU1 N2_nostop
 
Resource Report
Resource Website 		RRID:Addgene_168586 N2 Other Spectinomycin PMID:37267226 Backbone Marker:Rual et al, Genome Res. 14:2128-2135, 2004.; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Other; Bacterial Resistance:Spectinomycin Addgene 2026-10-10 02:04:39 0
pDONR223 HCoV-HKU1 NSP2_stop
 
Resource Report
Resource Website 		RRID:Addgene_168588 NSP2 Other Spectinomycin PMID:37267226 Backbone Marker:Rual et al, Genome Res. 14:2128-2135, 2004.; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Other; Bacterial Resistance:Spectinomycin Addgene 2026-10-10 02:04:39 0
pDONR223 HCoV-HKU1 E_nostop
 
Resource Report
Resource Website 		RRID:Addgene_168583 E Other Spectinomycin PMID:37267226 Backbone Marker:Rual et al, Genome Res. 14:2128-2135, 2004.; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Other; Bacterial Resistance:Spectinomycin Addgene 2026-10-10 02:04:39 0
pDONR223 HCoV-HKU1 M_nostop
 
Resource Report
Resource Website 		RRID:Addgene_168584 M Other Spectinomycin PMID:37267226 Backbone Marker:Rual et al, Genome Res. 14:2128-2135, 2004.; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Other; Bacterial Resistance:Spectinomycin Addgene 2026-10-10 02:04:39 0
pDONR223 HCoV-HKU1 N_nostop
 
Resource Report
Resource Website 		RRID:Addgene_168585 N Other Spectinomycin PMID:37267226 Backbone Marker:Rual et al, Genome Res. 14:2128-2135, 2004.; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Other; Bacterial Resistance:Spectinomycin Addgene 2026-10-10 02:04:39 0
Rosa26-LSL-PE2 Targeting Vector
 
Resource Report
Resource Website 		RRID:Addgene_196038 Cas9-MMLV-RT Synthetic Ampicillin PMID:37169967 Backbone Size:12710; Vector Backbone:LSL-Cas9-Rosa26TV; Vector Types:Mammalian Expression, Mouse Targeting; Bacterial Resistance:Ampicillin Addgene 2026-10-10 02:04:38 0
pDONR223 SARS-CoV-1 M_nostop
 
Resource Report
Resource Website 		RRID:Addgene_168536 M Other Spectinomycin PMID:37267226 Backbone Marker:Rual et al, Genome Res. 14:2128-2135, 2004.; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Other; Bacterial Resistance:Spectinomycin Addgene 2026-10-10 02:04:38 0
pDONR223 SARS-CoV-1 ORF6_nostop
 
Resource Report
Resource Website 		RRID:Addgene_168537 ORF6 Other Spectinomycin PMID:37267226 Backbone Marker:Rual et al, Genome Res. 14:2128-2135, 2004.; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Other; Bacterial Resistance:Spectinomycin Addgene 2026-10-10 02:04:38 0
pDONR223 SARS-CoV-1 ORF3a_nostop
 
Resource Report
Resource Website 		RRID:Addgene_168533 ORF3a Other Spectinomycin PMID:37267226 Backbone Marker:Rual et al, Genome Res. 14:2128-2135, 2004.; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Other; Bacterial Resistance:Spectinomycin Addgene 2026-10-10 02:04:38 0
pDONR223 SARS-CoV-1 ORF7b_nostop
 
Resource Report
Resource Website 		RRID:Addgene_168539 ORF7b Other Spectinomycin PMID:37267226 Backbone Marker:Rual et al, Genome Res. 14:2128-2135, 2004.; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Other; Bacterial Resistance:Spectinomycin Addgene 2026-10-10 02:04:38 0
pET32a-hEPGN
 
Resource Report
Resource Website 		RRID:Addgene_199232 Epithelial mitogen isoform 6 Homo sapiens Ampicillin PMID:35322149 Backbone Marker:EMD Biosciences; Backbone Size:5863; Vector Backbone:pET32a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin Addgene 2026-10-10 02:04:38 0
pDONR223 SARS-CoV-1 NSP8_nostop
 
Resource Report
Resource Website 		RRID:Addgene_168524 NSP8 Other Spectinomycin PMID:37267226 Backbone Marker:Rual et al, Genome Res. 14:2128-2135, 2004.; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Other; Bacterial Resistance:Spectinomycin Addgene 2026-10-10 02:04:38 0
pDONR223 SARS-CoV-1 NSP10_nostop
 
Resource Report
Resource Website 		RRID:Addgene_168526 NSP10 Other Spectinomycin PMID:37267226 Backbone Marker:Rual et al, Genome Res. 14:2128-2135, 2004.; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Other; Bacterial Resistance:Spectinomycin Addgene 2026-10-10 02:04:38 0
pDV06_feedback_entry_TagBFP-2A-MCS-2A-mNeonGreen-2A-MCP-ADAR2dd(E488Q)-NES
 
Resource Report
Resource Website 		RRID:Addgene_200402 Closed-loop DART VADAR sensor entry cassette Ampicillin PMID:36906659 Vector Backbone:Custom; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Ampicillin Addgene 2026-10-10 02:04:38 0
pDONR223 SARS-CoV-1 NSP4_nostop
 
Resource Report
Resource Website 		RRID:Addgene_168520 NSP4 Other Spectinomycin PMID:37267226 Backbone Marker:Rual et al, Genome Res. 14:2128-2135, 2004.; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Other; Bacterial Resistance:Spectinomycin Addgene 2026-10-10 02:04:38 0
pDONR223 SARS-CoV-1 NSP6_nostop
 
Resource Report
Resource Website 		RRID:Addgene_168522 NSP6 Other Spectinomycin PMID:37267226 Backbone Marker:Rual et al, Genome Res. 14:2128-2135, 2004.; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Other; Bacterial Resistance:Spectinomycin Addgene 2026-10-10 02:04:38 0
pDV17_pCMV-MCP-ADAR2dd(E488Q)-NES
 
Resource Report
Resource Website 		RRID:Addgene_200406 MCP-ADAR2dd(E488Q)-NES Synthetic Ampicillin PMID:36906659 Vector Backbone:Custom; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Ampicillin E488Q (hyperactive) Addgene 2026-10-10 02:04:38 0
pDV05.0_pEF1a-iRFP720
 
Resource Report
Resource Website 		RRID:Addgene_200407 iRFP720 Ampicillin PMID:36906659 Vector Backbone:Custom; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Ampicillin Addgene 2026-10-10 02:04:38 0

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