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Vector Backbone Description: Vector Backbone:N/A; Vector Types:; Bacterial Resistance:None
Comments: E. coli MEV15 is engineered to host sesquiterpenoid biosynthetic pathways. Sesquiterpenoid production is achieved by supplying the strain with plasmids encoding terpene cyclase and cytochrome P450s under the control of Marionette promoters (See https://www.addgene.org/kits/marionette-sensor-collection/ and 10.1038/s41589-018-0168-3). Sesquiterpenoid production can be induced by IPTG, vanillic aci, and other inducers controlling cytorhcome P450s. The strain has the upper MEV pathway from pMevT (addgene #17815) inserted into 4418413/4418414, the lower MEV pathway from pMBIS (addgene #17817) and E. coli ispA inserted into 4105665/4105664, the designed redox enzyme array inserteed into 3801913/3801912, and the Marionette cluster from sAJM.1506 (addgene #108254) inserted into 3753777/3752159 of E. coli BL21(DE3)'s genome. The nucleotide numbers are based on NCBI accession # NZ_CP053602. The redox enzyme array consists of fprD/fdxD from Streptomyces avermitilis, fpr/fldA from E. coli, fenr/fer1 from spinach chloroplasts, abd pdr/pdx (camA/camB) from Pseudomonas putida. The Marionette cluster was transferred using phage transduction, resulting in the replacement of nucleotides between 3745758/3839292 by those in the corresponding regions from sAJM.1506 (parent strain: E. coli MG1655), as evident by whole-genome sequencing.
Proper citation: RRID:Addgene_197112 Copy
Species: Other
Genetic Insert: NSP2
Vector Backbone Description: Backbone Marker:Rual et al, Genome Res. 14:2128-2135, 2004.; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Other; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:37267226
Proper citation: RRID:Addgene_168519 Copy
Species: Other
Genetic Insert: N2
Vector Backbone Description: Backbone Marker:Rual et al, Genome Res. 14:2128-2135, 2004.; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Other; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:37267226
Proper citation: RRID:Addgene_168586 Copy
Species: Other
Genetic Insert: NSP2
Vector Backbone Description: Backbone Marker:Rual et al, Genome Res. 14:2128-2135, 2004.; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Other; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:37267226
Proper citation: RRID:Addgene_168588 Copy
Species: Other
Genetic Insert: E
Vector Backbone Description: Backbone Marker:Rual et al, Genome Res. 14:2128-2135, 2004.; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Other; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:37267226
Proper citation: RRID:Addgene_168583 Copy
Species: Other
Genetic Insert: M
Vector Backbone Description: Backbone Marker:Rual et al, Genome Res. 14:2128-2135, 2004.; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Other; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:37267226
Proper citation: RRID:Addgene_168584 Copy
Species: Other
Genetic Insert: N
Vector Backbone Description: Backbone Marker:Rual et al, Genome Res. 14:2128-2135, 2004.; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Other; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:37267226
Proper citation: RRID:Addgene_168585 Copy
Species: Synthetic
Genetic Insert: Cas9-MMLV-RT
Vector Backbone Description: Backbone Size:12710; Vector Backbone:LSL-Cas9-Rosa26TV; Vector Types:Mammalian Expression, Mouse Targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37169967
Proper citation: RRID:Addgene_196038 Copy
Species: Other
Genetic Insert: M
Vector Backbone Description: Backbone Marker:Rual et al, Genome Res. 14:2128-2135, 2004.; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Other; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:37267226
Proper citation: RRID:Addgene_168536 Copy
Species: Other
Genetic Insert: ORF6
Vector Backbone Description: Backbone Marker:Rual et al, Genome Res. 14:2128-2135, 2004.; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Other; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:37267226
Proper citation: RRID:Addgene_168537 Copy
Species: Other
Genetic Insert: ORF3a
Vector Backbone Description: Backbone Marker:Rual et al, Genome Res. 14:2128-2135, 2004.; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Other; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:37267226
Proper citation: RRID:Addgene_168533 Copy
Species: Other
Genetic Insert: ORF7b
Vector Backbone Description: Backbone Marker:Rual et al, Genome Res. 14:2128-2135, 2004.; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Other; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:37267226
Proper citation: RRID:Addgene_168539 Copy
Species: Homo sapiens
Genetic Insert: Epithelial mitogen isoform 6
Vector Backbone Description: Backbone Marker:EMD Biosciences; Backbone Size:5863; Vector Backbone:pET32a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35322149
Proper citation: RRID:Addgene_199232 Copy
Species: Other
Genetic Insert: NSP8
Vector Backbone Description: Backbone Marker:Rual et al, Genome Res. 14:2128-2135, 2004.; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Other; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:37267226
Proper citation: RRID:Addgene_168524 Copy
Species: Other
Genetic Insert: NSP10
Vector Backbone Description: Backbone Marker:Rual et al, Genome Res. 14:2128-2135, 2004.; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Other; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:37267226
Proper citation: RRID:Addgene_168526 Copy
Genetic Insert: Closed-loop DART VADAR sensor entry cassette
Vector Backbone Description: Vector Backbone:Custom; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36906659
Proper citation: RRID:Addgene_200402 Copy
Species: Other
Genetic Insert: NSP4
Vector Backbone Description: Backbone Marker:Rual et al, Genome Res. 14:2128-2135, 2004.; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Other; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:37267226
Proper citation: RRID:Addgene_168520 Copy
Species: Other
Genetic Insert: NSP6
Vector Backbone Description: Backbone Marker:Rual et al, Genome Res. 14:2128-2135, 2004.; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Other; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:37267226
Proper citation: RRID:Addgene_168522 Copy
Species: Synthetic
Genetic Insert: MCP-ADAR2dd(E488Q)-NES
Vector Backbone Description: Vector Backbone:Custom; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36906659
Proper citation: RRID:Addgene_200406 Copy
Genetic Insert: iRFP720
Vector Backbone Description: Vector Backbone:Custom; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36906659
Proper citation: RRID:Addgene_200407 Copy
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