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Species: Homo sapiens
Genetic Insert: shREV3-4
Vector Backbone Description: Backbone Marker:Trono Laboratory; Backbone Size:11085; Vector Backbone:pLVTHM; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22028621
Comments: A versatile tool for conditional gene expression and knockdown.
Szulc J, Wiznerowicz M, Sauvain MO, Trono D, Aebischer P.
Nat Methods. 2006 Feb;3(2):109-16.
PMID:16432520
Proper citation: RRID:Addgene_38033 Copy
Genetic Insert: See comments
Vector Backbone Description: Vector Backbone:N/A; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:17220888
Comments: Host strain for pHL32B
Proper citation: RRID:Addgene_37976 Copy
Genetic Insert: See comments
Vector Backbone Description: Vector Backbone:N/A; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:17220888
Comments: Host strain for pHL32B
Strain description: ΔDam
Chromosomal changes: ΔDam
Proper citation: RRID:Addgene_37977 Copy
Genetic Insert: See comments
Vector Backbone Description: Vector Backbone:N/A; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:17220888
Comments: Host strain for pHL32B
Strain description: Δ(Dam+OxyR)
Chromosomal changes: Δ(Dam+OxyR)
Proper citation: RRID:Addgene_37979 Copy
Species: Begomovirus
Genetic Insert: pJRTCLCrVA.009
Vector Backbone Description: Backbone Marker:Cambia.org; Backbone Size:8935; Vector Backbone:pCAMBIA 1300; Vector Types:Agrobacterium Binary; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22853641
Proper citation: RRID:Addgene_37972 Copy
Species: Homo sapiens
Genetic Insert: E2F3a
Vector Backbone Description: Backbone Marker:Vogelstein Lab ; Backbone Size:6600; Vector Backbone:pCMV-Neo-Bam; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8246996
Comments: E2Fs bind DNA as a heterodimer with members of the DP family of transcription factors.
The 5' end of the coding sequence can be difficult to sequence.
Proper citation: RRID:Addgene_37970 Copy
Species: Homo sapiens
Genetic Insert: E2F6
Vector Backbone Description: Backbone Marker:Vogelstein Lab ; Backbone Size:6600; Vector Backbone:pCMV-Neo-Bam; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11171983
Comments: The backbone plasmid pCMV-Neo-Bam is Addgene#16440
E2Fs bind DNA as a heterodimer with members of the DP family of transcription factors.
Proper citation: RRID:Addgene_37965 Copy
Species: Homo sapiens
Genetic Insert: DP1
Vector Backbone Description: Backbone Marker:Vogelstein Lab ; Backbone Size:6600; Vector Backbone:pCMV-Neo-Bam; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8657117
Comments: DP1 functions as a heterodimer with several members of the E2F family of transcription factors.
Proper citation: RRID:Addgene_37968 Copy
Species: H. Herpesvirus 4 (EBV)
Genetic Insert: LF2
Vector Backbone Description: Backbone Size:8162; Vector Backbone:MSCV-N FLAGHA; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22810586
Comments: Note that the Gateway cloning reaction has left several vector-based nucleotides in frame with the 3' end of the insert, adding several amino acid residues [FLYKVVR] fused to the C-terminus of translated protein.
Proper citation: RRID:Addgene_37961 Copy
Species: H. Herpesvirus 4 (EBV)
Genetic Insert: LMP1
Vector Backbone Description: Backbone Size:8162; Vector Backbone:MSCV-N FLAGHA; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22810586
Proper citation: RRID:Addgene_37962 Copy
Species: H. Herpesvirus 4 (EBV)
Genetic Insert: LMP2A
Vector Backbone Description: Backbone Size:8162; Vector Backbone:MSCV-N FLAGHA; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22810586
Comments: Note that the Gateway cloning reaction has left several vector-based nucleotides in frame with the 3' end of the insert, adding several amino acid residues [FLYKVVR] fused to the C-terminus of translated protein.
Proper citation: RRID:Addgene_37963 Copy
Species: H. Herpesvirus 4 (EBV)
Genetic Insert: SM
Vector Backbone Description: Backbone Size:8162; Vector Backbone:MSCV-N FLAGHA; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22810586
Comments: Note that the Gateway cloning reaction has left several vector-based nucleotides in frame with the 3' end of the insert, adding several amino acid residues [FLYKVVR] fused to the C-terminus of translated protein.
Proper citation: RRID:Addgene_37964 Copy
Species: Mus musculus
Genetic Insert: Clathrin, light polypeptide (LCa)
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEYFP-N1; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21552254
Comments: Note: The clathrin light chain has an A9D amino acid residue substitution. This is a natural variant that does not affect protein function.
Proper citation: RRID:Addgene_38012 Copy
Species: H. Herpesvirus 4 (EBV)
Genetic Insert: LF1
Vector Backbone Description: Backbone Size:8162; Vector Backbone:MSCV-C FLAGHA; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22810586
Comments: Note that there is a P463T mutation which is not known to affect function.
Proper citation: RRID:Addgene_37960 Copy
Species: Mus musculus
Genetic Insert: Clathrin, light polypeptide (LCa)
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEYFP-N1; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21552254
Comments: Note: The clathrin light chain has an A9D amino acid residue substitution. This is a natural variant that does not affect protein function.
Proper citation: RRID:Addgene_38010 Copy
Species: Mus musculus
Genetic Insert: Clathrin, light polypeptide (LCa)
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEYFP-N1; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21552254
Comments: Note: The clathrin light chain has an A9D amino acid residue substitution. This is a natural variant that does not affect protein function.
Proper citation: RRID:Addgene_38011 Copy
Species: H. Herpesvirus 4 (EBV)
Genetic Insert: EBNA1
Vector Backbone Description: Backbone Size:8162; Vector Backbone:MSCV-N FLAGHA; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22810586
Comments: Note that the Gateway cloning reaction has left several vector-based nucleotides in frame with the 3' end of the insert, leaving additional amino acid residues [PTFLYKVVR] fused to the C-terminus of translated protein.
Proper citation: RRID:Addgene_37954 Copy
Species: Mus musculus
Genetic Insert: Clathrin, light polypeptide (LCa)
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4000; Vector Backbone:pEYFP-C1; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21552254
Comments: Note: The clathrin light chain has an A9D amino acid residue substitution. This is a natural variant that does not affect protein function. It is also missing its stop codon, so the peptide "PGPGSTGSR*" from the backbone is appended to the C-terminus of the CLC. The affect of this peptide on protein function is unknown.
Proper citation: RRID:Addgene_38009 Copy
Species: H. Herpesvirus 4 (EBV)
Genetic Insert: EBNA3B
Vector Backbone Description: Backbone Size:8162; Vector Backbone:MSCV-N FLAGHA; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22810586
Comments: Note that the Gateway cloning reaction has left several vector-based nucleotides in frame with the 3' end of the insert, adding several amino acid residues [FLYKVVR] fused to the C-terminus of translated protein.
Proper citation: RRID:Addgene_37957 Copy
Species: Synthetic
Genetic Insert: MBP (Maltose binding protein)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5200; Vector Backbone:FastBac-Dual; Vector Types:Insect Expression, Baculovirus expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_38008 Copy
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