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Species: Synthetic
Genetic Insert: mRuby2_smFP_OLLAS
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pCAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25915120
Proper citation: RRID:Addgene_59761 Copy
Species: Other
Genetic Insert: Cas9
Vector Backbone Description: Backbone Size:6215; Vector Backbone:pMZ283; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25352017
Comments: Please note that some batches of CspCI from New England Biolabs may cut inefficiently and alters the mobility of the DNA, possibly because it remains bound to the target. The two batches that are known to work are 0041303 and 0051407. This may be due to the particular target sequence present in pMZ283 and pMZ374.
Proper citation: RRID:Addgene_59896 Copy
Species: Other
Genetic Insert: Cas9
Vector Backbone Description: Backbone Size:6215; Vector Backbone:pMZ283; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25352017
Proper citation: RRID:Addgene_59898 Copy
Species: Other
Genetic Insert: Cas9
Vector Backbone Description: Backbone Size:6215; Vector Backbone:pMZ283; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25352017
Proper citation: RRID:Addgene_59897 Copy
Species: Homo sapiens
Genetic Insert: Bub1
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20124418
Proper citation: RRID:Addgene_59812 Copy
Species: Caenorhabditis elegans
Genetic Insert: unc-109(n499) gRNA
Vector Backbone Description: Vector Backbone:pRB1017; Vector Types:CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25161212
Proper citation: RRID:Addgene_59932 Copy
Species: Caenorhabditis elegans
Genetic Insert: sqt-1(e1350) gRNA2
Vector Backbone Description: Vector Backbone:pRB1017; Vector Types:CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25161212
Proper citation: RRID:Addgene_59935 Copy
Species: Synthetic
Genetic Insert: mCherry with intron containing the mouse mir-124–3
Vector Backbone Description: Vector Backbone:pcDNA5 FRT/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24847681
Comments: To create a repressive regulatory link between the miRNA and the mCherry transcript, we added a truncated version of the mir-124-regulated 3′UTR of the Vamp3 gene to the mRNA. This 3′UTR contains one 6-mer and three 7-mer target sites complementary to the mir-124 seed region (nucleotides 2−8 from the 5′ end of the miRNA). To better observe the dynamics of gene expression, we destabilized the mCherry protein using a standard PEST degradation tag.
Proper citation: RRID:Addgene_59892 Copy
Species: Synthetic
Genetic Insert: mCherry with intron containing the mouse mir-124–3
Vector Backbone Description: Vector Backbone:pcDNA5 FRT/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24847681
Comments: To create a repressive regulatory link between the miRNA and the mCherry transcript, we added a truncated version of the mir-124-regulated 3′UTR of the Vamp3 gene to the mRNA. This 3′UTR contains one 6-mer and three 7-mer target sites complementary to the mir-124 seed region (nucleotides 2−8 from the 5′ end of the miRNA). To better observe the dynamics of gene expression, we destabilized the mCherry protein using a standard PEST degradation tag.
Proper citation: RRID:Addgene_59894 Copy
Vector Backbone Description: Vector Backbone:pRB1017; Vector Types:Worm Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25161212
Proper citation: RRID:Addgene_59936 Copy
Species: Caenorhabditis elegans
Genetic Insert: rab-3 promoter
Vector Backbone Description: Vector Backbone:pCFJ150; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25377555
Proper citation: RRID:Addgene_59788 Copy
Species: Caenorhabditis elegans
Genetic Insert: rab-3 promoter
Vector Backbone Description: Vector Backbone:pCFJ150; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25377555
Proper citation: RRID:Addgene_59787 Copy
Genetic Insert: dihydropterine reductase
Vector Backbone Description: Backbone Size:3550; Vector Backbone:pBbE1k; Vector Types:; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22948011
Proper citation: RRID:Addgene_59945 Copy
Species: Homo sapiens
Genetic Insert: Separase
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17604273
Proper citation: RRID:Addgene_59827 Copy
Species: Synthetic
Genetic Insert: sfGFP
Vector Backbone Description: Vector Backbone:pZS2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25080493
Proper citation: RRID:Addgene_59948 Copy
Species: Synthetic
Genetic Insert: Splitposon w/ KanR, LacI & PTac
Vector Backbone Description: Vector Backbone:pSB1C3; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:25080493
Proper citation: RRID:Addgene_59947 Copy
Species: Aedes aegypti
Genetic Insert: Aedes aegypti Or4 cDNA allele G
Vector Backbone Description: Backbone Size:8489; Vector Backbone:pUASTattB; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25391959
Proper citation: RRID:Addgene_59798 Copy
Species: Synthetic
Genetic Insert: Three-fragment T7* RNA polymerase
Vector Backbone Description: Vector Backbone:pSB3C5; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:25080493
Proper citation: RRID:Addgene_59952 Copy
Species: Homo sapiens
Genetic Insert: p31
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22100920
Proper citation: RRID:Addgene_59833 Copy
Species: Other
Genetic Insert: ALIPHATIC AMIDASE
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:3582; Vector Backbone:pET-29b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25286161
Proper citation: RRID:Addgene_59836 Copy
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